Potassium in PDB 5tkr: Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant

The structure of Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant, PDB code: 5tkr was solved by J.P.Bacik, J.R.Klesmith, R.Michalczyk, T.A.Whitehead, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.08 / 1.80
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	70.060, 70.060, 261.770, 90.00, 90.00, 90.00
R / Rfree (%)	17.5 / 20.8

The structure of Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant (pdb code 5tkr). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant, PDB code: 5tkr:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:K505 b:23.2 occ:1.00 OD1 A:ASN436 2.7 20.3 1.0 OD1 A:ASN437 2.7 22.1 1.0 O A:HOH948 2.8 27.3 1.0 O A:HIS176 2.8 20.1 1.0 O A:HOH831 2.9 20.6 1.0 O A:LEU174 3.1 19.2 1.0 CG A:ASN436 3.5 21.2 1.0 CG A:ASN437 3.6 23.4 1.0 C A:LEU175 3.7 21.8 1.0 C A:HIS176 3.8 22.6 1.0 O A:LEU175 3.8 21.1 1.0 ND2 A:ASN436 3.9 18.3 1.0 N A:HIS176 4.0 20.3 1.0 ND2 A:ASN437 4.0 22.1 1.0 C A:LEU174 4.1 20.0 1.0 CA A:LEU175 4.2 20.1 1.0 O A:HOH667 4.2 22.9 1.0 O A:HOH970 4.2 20.7 1.0 O A:HOH961 4.3 36.8 1.0 NH1 A:ARG182 4.3 21.3 1.0 N A:LEU175 4.5 17.8 1.0 CA A:HIS176 4.5 19.0 1.0 CD2 A:HIS176 4.5 23.4 1.0 O A:HOH659 4.6 25.5 1.0 C A:ASN436 4.7 19.9 1.0 N A:HIS177 4.7 20.9 1.0 N A:ASN437 4.7 19.9 1.0 CG A:HIS176 4.8 23.0 1.0 NE2 A:HIS176 4.8 24.9 1.0 CB A:ASN437 4.8 20.0 1.0 CA A:HIS177 4.8 20.9 1.0 CB A:ASN436 4.8 19.2 1.0 N A:ASN436 4.9 19.3 1.0 O A:ASN436 4.9 20.5 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of A Lipomyces Starkeyi Levoglucosan Kinase G359R Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:K506 b:24.3 occ:1.00 O A:HOH700 2.6 20.6 1.0 OE2 A:GLU53 2.8 25.2 1.0 O A:HOH696 2.8 26.9 1.0 OD2 A:ASP29 2.9 22.8 1.0 O A:ARG359 3.0 21.4 1.0 OD1 A:ASP29 3.1 21.6 1.0 O2A A:ADP501 3.2 19.5 1.0 CG A:ASP29 3.2 22.9 1.0 O A:HOH658 3.5 16.6 1.0 CD A:GLU53 3.6 23.9 1.0 OH A:TYR51 3.6 21.0 1.0 O A:HOH735 3.7 17.5 1.0 O1A A:ADP501 3.7 19.5 1.0 C A:ARG359 4.0 19.6 1.0 OE1 A:GLU53 4.0 25.4 1.0 O A:HOH785 4.0 19.4 1.0 PA A:ADP501 4.1 19.5 1.0 CB A:ARG359 4.2 27.4 1.0 CA A:ARG359 4.2 22.3 1.0 MG A:MG502 4.2 19.5 1.0 CB A:ASP29 4.5 18.2 1.0 CG A:ARG359 4.6 30.9 1.0 CG A:GLU53 4.7 20.7 1.0 O A:HOH838 4.8 18.1 1.0 OE1 A:GLU362 4.8 18.7 1.0 CZ A:TYR51 4.9 19.7 1.0 CD A:ARG359 4.9 34.6 1.0 O5' A:ADP501 5.0 21.1 1.0

J.R.Klesmith, J.P.Bacik, E.E.Wrenbeck, R.Michalczyk, T.A.Whitehead. Trade-Offs Between Enzyme Fitness and Solubility Illuminated By Deep Mutational Scanning. Proc. Natl. Acad. Sci. V. 114 2265 2017U.S.A..
ISSN: ESSN 1091-6490
PubMed: 28196882
DOI: 10.1073/PNAS.1614437114