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Potassium in PDB 5t5n: Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium

Protein crystallography data

The structure of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium, PDB code: 5t5n was solved by S.B.Long, G.Vaisey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.95 / 3.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 98.243, 243.878, 172.052, 90.00, 93.84, 90.00
R / Rfree (%) 21.8 / 24.3

Other elements in 5t5n:

The structure of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium also contains other interesting chemical elements:

Chlorine (Cl) 15 atoms
Calcium (Ca) 5 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium (pdb code 5t5n). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 5 binding sites of Potassium where determined in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium, PDB code: 5t5n:
Jump to Potassium binding site number: 1; 2; 3; 4; 5;

Potassium binding site 1 out of 5 in 5t5n

Go back to Potassium Binding Sites List in 5t5n
Potassium binding site 1 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K503

b:91.5
occ:1.00
OH B:TYR245 2.8 0.1 1.0
O A:LEU14 2.9 0.6 1.0
OG A:SER18 3.1 94.8 1.0
OE2 B:GLU292 3.5 94.7 1.0
OE2 B:GLU35 3.5 82.8 1.0
C A:LEU14 4.0 85.0 1.0
N A:SER18 4.1 92.1 1.0
CA A:GLY15 4.2 74.8 1.0
CZ B:TYR245 4.2 97.2 1.0
N A:PHE17 4.2 87.5 1.0
CD B:GLU292 4.3 84.9 1.0
CB A:SER18 4.3 75.4 1.0
N A:THR16 4.4 93.8 1.0
C A:GLY15 4.4 91.3 1.0
OE1 B:GLU292 4.4 97.8 1.0
CD B:GLU35 4.5 98.7 1.0
CG B:GLU35 4.5 80.9 1.0
N A:GLY15 4.5 55.9 1.0
CB A:PHE17 4.6 77.4 1.0
O A:ARG13 4.7 74.6 1.0
CA A:PHE17 4.8 74.8 1.0
CA A:SER18 4.8 81.2 1.0
NZ B:LYS289 4.9 64.5 1.0
C A:PHE17 4.9 77.9 1.0
CE2 B:TYR245 5.0 94.0 1.0

Potassium binding site 2 out of 5 in 5t5n

Go back to Potassium Binding Sites List in 5t5n
Potassium binding site 2 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K507

b:95.4
occ:1.00
OH A:TYR245 2.7 90.2 1.0
O E:LEU14 2.9 1.0 1.0
OG E:SER18 3.3 0.9 1.0
OE2 A:GLU292 3.4 89.8 1.0
OE2 A:GLU35 3.5 0.8 1.0
CZ A:TYR245 4.1 0.4 1.0
C E:LEU14 4.1 95.5 1.0
CD A:GLU292 4.3 89.3 1.0
N E:SER18 4.3 77.5 1.0
CD A:GLU35 4.4 0.9 1.0
CG A:GLU35 4.4 84.1 1.0
OE1 A:GLU292 4.4 0.3 1.0
N E:PHE17 4.4 82.5 1.0
CA E:GLY15 4.4 76.0 1.0
N E:THR16 4.6 85.6 1.0
CB E:SER18 4.6 94.7 1.0
C E:GLY15 4.6 87.2 1.0
NZ A:LYS289 4.6 61.2 1.0
CB E:PHE17 4.7 76.0 1.0
N E:GLY15 4.7 65.1 1.0
O E:ARG13 4.7 86.7 1.0
CE1 A:TYR245 4.8 0.1 1.0
CE A:LYS289 4.9 57.0 1.0
CE2 A:TYR245 4.9 0.2 1.0
CA E:PHE17 4.9 77.3 1.0

Potassium binding site 3 out of 5 in 5t5n

Go back to Potassium Binding Sites List in 5t5n
Potassium binding site 3 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K504

b:92.6
occ:1.00
OH C:TYR245 2.7 85.8 1.0
O B:LEU14 3.0 85.3 1.0
OG B:SER18 3.4 91.3 1.0
OE2 C:GLU292 3.4 79.4 1.0
OE2 C:GLU35 3.5 86.9 1.0
CZ C:TYR245 4.1 98.0 1.0
C B:LEU14 4.1 83.1 1.0
CD C:GLU292 4.2 82.2 1.0
OE1 C:GLU292 4.3 87.1 1.0
N B:SER18 4.3 84.1 1.0
CD C:GLU35 4.4 98.1 1.0
CG C:GLU35 4.4 81.5 1.0
CA B:GLY15 4.4 82.9 1.0
N B:PHE17 4.4 87.9 1.0
N B:THR16 4.6 0.3 1.0
CB B:SER18 4.6 68.0 1.0
C B:GLY15 4.7 89.7 1.0
CB B:PHE17 4.7 80.7 1.0
N B:GLY15 4.7 71.2 1.0
NZ C:LYS289 4.8 66.8 1.0
O B:ARG13 4.8 73.8 1.0
CE1 C:TYR245 4.8 93.2 1.0
CE2 C:TYR245 4.9 94.4 1.0
CE C:LYS289 4.9 67.9 1.0
CA B:PHE17 5.0 80.5 1.0

Potassium binding site 4 out of 5 in 5t5n

Go back to Potassium Binding Sites List in 5t5n
Potassium binding site 4 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K507

b:92.0
occ:1.00
OH D:TYR245 2.8 81.1 1.0
O C:LEU14 2.9 0.6 1.0
OG C:SER18 3.3 93.5 1.0
OE2 D:GLU292 3.4 73.9 1.0
OE2 D:GLU35 3.5 84.4 1.0
C C:LEU14 4.0 88.2 1.0
CZ D:TYR245 4.2 92.7 1.0
N C:SER18 4.2 82.4 1.0
CD D:GLU292 4.2 74.4 1.0
N C:PHE17 4.2 77.1 1.0
CA C:GLY15 4.2 78.2 1.0
OE1 D:GLU292 4.4 92.1 1.0
N C:THR16 4.4 82.5 1.0
CD D:GLU35 4.5 97.8 1.0
C C:GLY15 4.5 85.9 1.0
CB C:SER18 4.5 78.4 1.0
CG D:GLU35 4.5 80.3 1.0
N C:GLY15 4.6 60.9 1.0
CB C:PHE17 4.6 70.1 1.0
O C:ARG13 4.7 65.7 1.0
NZ D:LYS289 4.7 66.0 1.0
CA C:PHE17 4.8 75.9 1.0
CE D:LYS289 5.0 57.0 1.0
CE1 D:TYR245 5.0 92.6 1.0
CA C:SER18 5.0 90.0 1.0
C C:PHE17 5.0 78.8 1.0
CE2 D:TYR245 5.0 96.5 1.0

Potassium binding site 5 out of 5 in 5t5n

Go back to Potassium Binding Sites List in 5t5n
Potassium binding site 5 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K504

b:0.4
occ:1.00
OH E:TYR245 2.8 81.4 1.0
O D:LEU14 2.9 86.6 1.0
OG D:SER18 3.2 0.7 1.0
OE2 E:GLU292 3.4 82.3 1.0
OE2 E:GLU35 3.4 89.7 1.0
C D:LEU14 4.0 88.2 1.0
CZ E:TYR245 4.1 0.2 1.0
N D:SER18 4.2 92.4 1.0
CA D:GLY15 4.2 83.8 1.0
N D:PHE17 4.2 81.3 1.0
CD E:GLU292 4.3 79.5 1.0
CD E:GLU35 4.4 96.2 1.0
N D:THR16 4.4 87.1 1.0
OE1 E:GLU292 4.4 84.9 1.0
CB D:SER18 4.4 80.3 1.0
CG E:GLU35 4.5 70.1 1.0
C D:GLY15 4.5 93.4 1.0
N D:GLY15 4.6 66.3 1.0
CB D:PHE17 4.6 67.3 1.0
O D:ARG13 4.7 74.7 1.0
NZ E:LYS289 4.8 63.9 1.0
CA D:PHE17 4.8 75.0 1.0
CA D:SER18 4.9 77.3 1.0
CE2 E:TYR245 4.9 89.3 1.0
CE1 E:TYR245 5.0 97.9 1.0
C D:PHE17 5.0 80.0 1.0
CE E:LYS289 5.0 52.7 1.0

Reference:

G.Vaisey, A.N.Miller, S.B.Long. Distinct Regions That Control Ion Selectivity and Calcium-Dependent Activation in the Bestrophin Ion Channel. Proc. Natl. Acad. Sci. V. 113 E7399 2016U.S.A..
ISSN: ESSN 1091-6490
PubMed: 27821745
DOI: 10.1073/PNAS.1614688113
Page generated: Mon Dec 14 00:07:50 2020

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