Potassium in PDB 5osn: Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography

The structure of Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography, PDB code: 5osn was solved by P.Roedig, H.M.Ginn, T.Pakendorf, G.Sutton, K.Harlos, T.S.Walter, J.Meyer, P.Fischer, R.Duman, I.Vartiainen, B.Reime, M.Warmer, A.S.Brewster, I.D.Young, T.Michels-Clark, N.K.Sauter, A.Kotecha, J.Kelly, D.J.Rowlands, M.Sikorsky, S.Nelson, D.S.Damiani, R.Alonso-Mori, J.Ren, E.E.Fry, C.David, D.I.Stuart, A.Wagner, A.Meents, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.71 / 2.30
Space group	F 2 3 2
Cell size a, b, c (Å), α, β, γ (°)	436.600, 436.600, 436.600, 90.00, 90.00, 90.00
R / Rfree (%)	23.3 / 25.7

The binding sites of Potassium atom in the Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography (pdb code 5osn). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography, PDB code: 5osn:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ A:K302 b:44.8 occ:1.00 O A:ASN57 2.9 40.0 1.0 O A:VAL15 2.9 30.2 1.0 OD1 A:ASN17 2.9 26.0 1.0 OG1 A:THR14 3.1 44.3 1.0 O A:HOH510 3.4 43.7 1.0 CB A:THR14 3.6 19.4 1.0 O A:HOH487 3.6 36.1 1.0 CG A:ASN17 3.8 23.8 1.0 C A:VAL15 4.0 21.0 1.0 C A:ASN57 4.1 32.3 1.0 N A:ASN17 4.3 14.4 1.0 ND2 A:ASN17 4.3 22.4 1.0 C A:THR14 4.5 34.6 1.0 CG2 A:THR14 4.5 25.5 1.0 O A:THR14 4.6 22.6 1.0 C A:GLU16 4.6 28.8 1.0 CA A:GLU16 4.6 18.7 1.0 N A:VAL15 4.7 31.1 1.0 CA A:THR14 4.7 35.1 1.0 N A:ASN57 4.8 19.7 1.0 N A:GLU16 4.8 38.8 1.0 CA A:ASN57 4.8 15.3 1.0 CA A:ASN17 4.9 37.6 1.0 CB A:ASN17 4.9 26.0 1.0 CB A:ASN57 5.0 34.0 1.0 CA A:THR58 5.0 28.7 1.0

Potassium binding site 2 out of 3 in the Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ A:K303 b:40.4 occ:1.00 O D:ALA65 2.7 35.0 1.0 O A:LEU33 2.8 33.7 1.0 O D:GLU63 2.8 37.9 1.0 O A:THR30 3.3 25.6 1.0 NE2 A:GLN34 3.4 13.5 1.0 O A:PRO31 3.5 32.0 1.0 C D:GLU63 3.8 32.0 1.0 CA A:PRO31 3.8 21.9 1.0 C A:PRO31 3.8 31.4 1.0 C D:ALA65 3.9 28.9 1.0 C A:LEU33 3.9 33.7 1.0 O A:HOH488 4.0 44.8 1.0 O D:LYS62 4.1 42.5 1.0 CD A:GLN34 4.2 19.6 1.0 CA D:GLU63 4.2 31.9 1.0 C A:THR30 4.3 18.6 1.0 CD D:PRO67 4.3 22.2 1.0 CG D:PRO67 4.3 15.9 1.0 OD1 A:ASN43 4.3 35.4 1.0 N A:LEU33 4.4 33.8 1.0 N D:ALA65 4.5 43.8 1.0 N A:PRO31 4.5 35.2 1.0 CA A:LEU33 4.6 36.3 1.0 OE1 A:GLN34 4.7 49.2 1.0 CA D:VAL66 4.7 34.4 1.0 C D:ALA64 4.8 27.6 1.0 N D:ALA64 4.8 32.5 1.0 N A:ALA32 4.8 19.8 1.0 N D:VAL66 4.8 18.8 1.0 CA D:ALA65 4.8 13.4 1.0 CB A:LEU33 4.8 20.8 1.0 N D:PRO67 4.8 39.5 1.0 N A:GLN34 5.0 25.1 1.0 C D:LYS62 5.0 26.0 1.0

Potassium binding site 3 out of 3 in the Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Bovine Enterovirus 2 Determined with Serial Femtosecond X-Ray Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ A:K305 b:37.0 occ:1.00 OE1 C:GLN222 2.9 33.4 1.0 O C:HOH425 3.0 45.0 1.0 O A:SER42 3.1 28.6 1.0 O A:HOH415 3.1 49.5 1.0 OD2 C:ASP114 3.5 24.8 1.0 CB C:ASP114 3.9 25.9 1.0 CG C:ASP114 3.9 29.6 1.0 C A:SER42 4.0 34.4 1.0 CD C:GLN222 4.0 20.9 1.0 O C:HOH429 4.2 28.9 1.0 CG2 A:THR41 4.2 9.7 1.0 O C:ALA221 4.5 27.9 1.0 O A:ALA44 4.6 21.7 1.0 NE2 C:GLN222 4.6 31.4 1.0 CB A:SER42 4.7 19.0 1.0 CA A:ASN43 4.7 27.8 1.0 N A:ASN43 4.8 45.0 1.0 CA A:SER42 4.8 23.6 1.0 OD1 C:ASP114 4.8 16.7 1.0 OG A:SER42 4.9 37.4 1.0 N A:SER42 5.0 24.0 1.0

P.Roedig, H.M.Ginn, T.Pakendorf, G.Sutton, K.Harlos, T.S.Walter, J.Meyer, P.Fischer, R.Duman, I.Vartiainen, B.Reime, M.Warmer, A.S.Brewster, I.D.Young, T.Michels-Clark, N.K.Sauter, A.Kotecha, J.Kelly, D.J.Rowlands, M.Sikorsky, S.Nelson, D.S.Damiani, R.Alonso-Mori, J.Ren, E.E.Fry, C.David, D.I.Stuart, A.Wagner, A.Meents. High-Speed Fixed-Target Serial Virus Crystallography. Nat. Methods V. 14 805 2017.
ISSN: ESSN 1548-7105
PubMed: 28628129
DOI: 10.1038/NMETH.4335