Potassium in PDB 5mrp: Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2)

All present enzymatic activity of Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2):
2.7.7.60;

The structure of Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2), PDB code: 5mrp was solved by A.Schwab, B.Illarionov, A.Frank, A.Kunfermann, M.Seet, A.Bacher, M.Witschel, M.Fischer, M.Groll, F.Diederich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 1.90
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	74.990, 74.990, 221.720, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 23.5

The structure of Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2) also contains other interesting chemical elements:

Cadmium	(Cd)	3 atoms
Chlorine	(Cl)	1 atom

The binding sites of Potassium atom in the Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2) (pdb code 5mrp). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2), PDB code: 5mrp:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2) within 5.0Å range: probe atom residue distance (Å) B Occ A:K402 b:71.0 occ:1.00 OD2 A:ASP290 3.0 61.2 1.0 OD1 A:ASP290 3.2 67.2 1.0 CG A:ASP290 3.5 63.8 1.0 O A:LYS284 4.0 57.9 1.0 OG1 A:THR286 4.1 90.3 1.0 OG1 A:THR287 5.0 76.2 1.0 CB A:ASP290 5.0 65.4 1.0

Potassium binding site 2 out of 2 in the Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Arabidopsis Thaliana Ispd GLU258ALA Mutant in Complex with Azolopyrimidine (2) within 5.0Å range: probe atom residue distance (Å) B Occ A:K403 b:67.6 occ:1.00 O A:HOH515 3.1 45.3 1.0 ND1 A:HIS271 4.1 31.1 1.0 CB A:HIS271 4.2 28.6 1.0 CD2 A:LEU244 4.5 49.2 1.0 CG A:HIS271 4.6 28.4 1.0 N A:HIS271 4.7 37.1 1.0 CA A:HIS271 4.8 30.9 1.0 CE A:LYS247 4.9 77.2 1.0

A.Schwab, B.Illarionov, A.Frank, A.Kunfermann, M.Seet, A.Bacher, M.C.Witschel, M.Fischer, M.Groll, F.Diederich. Mechanism of Allosteric Inhibition of the Enzyme Ispd By Three Different Classes of Ligands. Acs Chem. Biol. V. 12 2132 2017.
ISSN: ESSN 1554-8937
PubMed: 28686408
DOI: 10.1021/ACSCHEMBIO.7B00004