Potassium in PDB 5mrn: Arabidopsis Thaliana Ispd GLU258ALA Mutant

All present enzymatic activity of Arabidopsis Thaliana Ispd GLU258ALA Mutant:
2.7.7.60;

The structure of Arabidopsis Thaliana Ispd GLU258ALA Mutant, PDB code: 5mrn was solved by A.Schwab, B.Illarionov, A.Frank, A.Kunfermann, M.Seet, A.Bacher, M.Witschel, M.Fischer, M.Groll, F.Diederich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.00
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	74.910, 74.910, 224.930, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 23.8

The structure of Arabidopsis Thaliana Ispd GLU258ALA Mutant also contains other interesting chemical elements:

Cadmium

(Cd)

3 atoms

The binding sites of Potassium atom in the Arabidopsis Thaliana Ispd GLU258ALA Mutant (pdb code 5mrn). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Arabidopsis Thaliana Ispd GLU258ALA Mutant, PDB code: 5mrn:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Arabidopsis Thaliana Ispd GLU258ALA Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Arabidopsis Thaliana Ispd GLU258ALA Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:K401 b:93.9 occ:1.00 OD2 A:ASP290 3.0 72.5 1.0 OG1 A:THR286 3.1 0.7 1.0 CG A:ASP290 3.8 82.9 1.0 OD1 A:ASP290 3.9 84.5 1.0 CB A:THR286 4.4 1.0 1.0 O A:LYS284 4.4 77.2 1.0 CG2 A:THR286 4.5 0.2 1.0 CG2 A:THR287 4.8 95.7 1.0 OG1 A:THR287 4.9 96.7 1.0

Potassium binding site 2 out of 2 in the Arabidopsis Thaliana Ispd GLU258ALA Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Arabidopsis Thaliana Ispd GLU258ALA Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:K402 b:59.6 occ:1.00 O A:MET119 2.7 44.3 1.0 O A:SER117 2.9 46.5 1.0 O A:ASP145 3.1 62.5 1.0 O A:VAL122 3.2 45.0 1.0 C A:MET119 3.5 45.6 1.0 CG1 A:VAL146 3.9 69.2 1.0 C A:SER117 3.9 47.2 1.0 C A:ASP145 4.0 73.1 1.0 N A:MET119 4.1 49.2 1.0 N A:PRO120 4.1 44.9 1.0 CA A:PRO120 4.2 44.1 1.0 C A:VAL122 4.3 43.1 1.0 C A:ARG118 4.4 51.4 1.0 CB A:ASP145 4.4 86.7 1.0 OD2 A:ASP145 4.4 83.8 1.0 CA A:MET119 4.4 46.7 1.0 O A:PHE116 4.5 37.7 1.0 C A:PRO120 4.5 44.2 1.0 CB A:VAL122 4.5 39.8 1.0 N A:VAL122 4.6 34.8 1.0 O A:PRO120 4.6 45.2 1.0 CG A:ASP145 4.7 89.1 1.0 CA A:SER117 4.7 47.4 1.0 CA A:VAL122 4.7 39.3 1.0 N A:ARG118 4.7 49.7 1.0 CA A:ASP145 4.8 76.9 1.0 O A:ARG118 4.8 54.0 1.0 CA A:ARG118 4.8 52.1 1.0 N A:VAL146 4.8 72.5 1.0

A.Schwab, B.Illarionov, A.Frank, A.Kunfermann, M.Seet, A.Bacher, M.C.Witschel, M.Fischer, M.Groll, F.Diederich. Mechanism of Allosteric Inhibition of the Enzyme Ispd By Three Different Classes of Ligands. Acs Chem. Biol. V. 12 2132 2017.
ISSN: ESSN 1554-8937
PubMed: 28686408
DOI: 10.1021/ACSCHEMBIO.7B00004