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Potassium in PDB 5icf: Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine

Enzymatic activity of Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine

All present enzymatic activity of Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine:
2.1.1.128;

Protein crystallography data

The structure of Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine, PDB code: 5icf was solved by A.Y.Robin, M.Graindorge, C.Giustini, R.Dumas, M.Matringe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.77 / 1.80
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 73.340, 109.090, 40.950, 90.00, 90.00, 90.00
R / Rfree (%) 15.6 / 19.2

Other elements in 5icf:

The structure of Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine also contains other interesting chemical elements:

Sodium (Na) 1 atom

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine (pdb code 5icf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine, PDB code: 5icf:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 5icf

Go back to Potassium Binding Sites List in 5icf
Potassium binding site 1 out of 2 in the Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K403

b:16.1
occ:0.50
O A:HOH605 3.0 11.0 1.0
NE2 A:HIS124 3.2 13.3 1.0
CE A:MET120 4.0 11.7 1.0
CD2 A:HIS124 4.1 11.2 1.0
O A:HOH621 4.1 25.0 0.5
CE1 A:HIS124 4.2 13.8 1.0
CB A:MET120 4.4 8.9 1.0
SD A:MET120 4.8 10.8 1.0

Potassium binding site 2 out of 2 in 5icf

Go back to Potassium Binding Sites List in 5icf
Potassium binding site 2 out of 2 in the Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of (S)-Norcoclaurine 6-O-Methyltransferase with S- Adenosyl-L-Homocysteine and Sanguinarine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K404

b:23.9
occ:1.00
OG1 A:THR297 3.1 12.1 1.0
N A:ASP289 3.2 16.8 1.0
NH1 A:ARG300 3.2 13.2 1.0
CB A:ASP289 3.6 23.1 1.0
CD A:ARG300 3.7 10.7 1.0
CB A:THR297 3.8 10.3 1.0
CA A:THR297 3.8 9.9 1.0
ND1 A:HIS294 3.9 17.9 1.0
CA A:ASP289 4.0 21.6 1.0
CG2 A:THR297 4.0 10.7 1.0
C A:LEU288 4.2 15.6 1.0
CZ A:ARG300 4.3 14.5 1.0
O A:ILE287 4.3 15.1 1.0
CA A:LEU288 4.4 15.4 0.5
CA A:LEU288 4.4 15.6 0.5
NE A:ARG300 4.4 11.8 1.0
N A:THR297 4.5 8.9 1.0
CD1 A:LEU288 4.6 15.9 0.5
CG A:HIS294 4.6 14.9 1.0
CB A:HIS294 4.6 12.0 1.0
O A:ASP289 4.7 27.6 1.0
CE1 A:HIS294 4.8 16.5 1.0
C A:ASP289 4.8 22.3 1.0
CB A:ARG300 4.8 10.0 1.0
O A:SER292 4.8 18.5 1.0
CG A:ARG300 4.8 10.3 1.0
C A:THR297 5.0 9.9 1.0

Reference:

A.Y.Robin, C.Giustini, M.Graindorge, M.Matringe, R.Dumas. Crystal Structure of Norcoclaurine-6-O-Methyltransferase, A Key Rate-Limiting Step in the Synthesis of Benzylisoquinoline Alkaloids. Plant J. V. 87 641 2016.
ISSN: ESSN 1365-313X
PubMed: 27232113
DOI: 10.1111/TPJ.13225
Page generated: Sun Dec 13 23:59:37 2020

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