Potassium in PDB 5hhk: Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met)

The structure of Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met), PDB code: 5hhk was solved by C.Zhao, A.M.Pyle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.06 / 1.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	42.010, 88.120, 79.670, 90.00, 95.45, 90.00
R / Rfree (%)	15.7 / 18.9

The binding sites of Potassium atom in the Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met) (pdb code 5hhk). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met), PDB code: 5hhk:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met) within 5.0Å range: probe atom residue distance (Å) B Occ A:K404 b:23.8 occ:1.00 OD1 A:ASP151 2.6 18.8 1.0 OD1 A:ASP239 2.7 13.2 1.0 O A:ILE152 2.7 14.2 1.0 O A:CYS240 2.8 10.3 1.0 O A:HOH655 3.0 22.1 1.0 O A:HOH774 3.2 19.5 1.0 O A:HOH704 3.3 12.9 1.0 C A:ILE152 3.7 13.1 1.0 N A:CYS240 3.7 9.3 1.0 C A:CYS240 3.8 9.4 1.0 CG A:ASP239 3.9 13.2 1.0 CG A:ASP151 3.9 17.6 1.0 N A:ILE152 4.0 11.9 1.0 O A:HOH769 4.1 14.8 1.0 C A:ASP151 4.2 11.2 1.0 CG2 A:ILE241 4.3 9.3 1.0 O A:HOH728 4.4 30.3 1.0 C A:ASP239 4.4 9.3 1.0 CA A:ASP151 4.4 11.2 1.0 CA A:ASP239 4.4 10.2 1.0 CA A:CYS240 4.4 10.2 0.2 CA A:CYS240 4.4 9.3 0.8 CA A:ILE152 4.5 13.1 1.0 O A:HOH709 4.5 14.4 1.0 N A:ASP153 4.5 13.1 1.0 O A:HOH568 4.6 37.5 1.0 OD2 A:ASP239 4.7 14.1 1.0 CA A:ASP153 4.7 14.0 1.0 CB A:ASP151 4.7 13.7 1.0 N A:ILE241 4.7 8.6 1.0 OD2 A:ASP151 4.7 19.4 1.0 CB A:ASP239 4.8 11.7 1.0 O A:ASP151 4.9 11.2 1.0 O A:HOH508 4.9 25.9 1.0

Potassium binding site 2 out of 2 in the Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Reverse Transcriptase Domain of Group II Intron Maturase From Roseburia Intestinalis in P21 Space Group (Se-Met) within 5.0Å range: probe atom residue distance (Å) B Occ B:K403 b:30.9 occ:1.00 OD1 B:ASP151 2.6 21.7 1.0 OD1 B:ASP239 2.6 17.9 1.0 O B:ILE152 2.7 17.1 1.0 O B:CYS240 2.7 11.4 1.0 O B:HOH761 2.9 29.3 1.0 O B:HOH674 3.3 27.3 1.0 O B:HOH580 3.5 15.6 1.0 C B:ILE152 3.6 17.1 1.0 N B:CYS240 3.7 10.4 1.0 C B:CYS240 3.7 10.8 1.0 N B:ILE152 3.8 15.7 1.0 CG B:ASP239 3.8 16.8 1.0 CG B:ASP151 3.8 22.8 1.0 O B:HOH532 3.9 44.0 1.0 CG2 B:ILE241 4.1 9.4 1.0 C B:ASP151 4.1 14.0 1.0 O B:HOH721 4.2 19.9 1.0 C B:ASP239 4.3 11.2 1.0 CA B:CYS240 4.3 11.1 0.2 CA B:ILE152 4.3 18.6 1.0 CA B:CYS240 4.3 10.8 0.8 CA B:ASP239 4.3 12.1 1.0 CA B:ASP151 4.3 14.4 1.0 N B:ASP153 4.5 16.3 1.0 OD2 B:ASP239 4.6 18.6 1.0 OD2 B:ASP151 4.6 27.7 1.0 O B:HOH608 4.6 27.1 1.0 N B:ILE241 4.6 9.5 1.0 CB B:ASP239 4.7 13.7 1.0 CB B:ASP151 4.7 18.4 1.0 CA B:ASP153 4.7 16.6 1.0 CE1 B:TYR284 4.8 9.2 1.0 O B:ASP151 4.8 13.7 1.0 CD1 B:TYR284 4.9 8.8 1.0

C.Zhao, A.M.Pyle. Crystal Structures of A Group II Intron Maturase Reveal A Missing Link in Spliceosome Evolution. Nat.Struct.Mol.Biol. V. 23 558 2016.
ISSN: ESSN 1545-9985
PubMed: 27136328
DOI: 10.1038/NSMB.3224