Potassium in PDB 5fw5: Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives

All present enzymatic activity of Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives:
3.6.4.12; 3.6.4.13;

The structure of Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives, PDB code: 5fw5 was solved by T.Schulte, L.Liu, M.D.Panas, B.Thaa, B.Goette, A.Achour, G.M.Mcinerney, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.49 / 1.92
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	94.975, 94.975, 107.593, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 20.2

The binding sites of Potassium atom in the Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives (pdb code 5fw5). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives, PDB code: 5fw5:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives within 5.0Å range: probe atom residue distance (Å) B Occ B:K1143 b:72.8 occ:1.00 H B:ASP28 2.2 28.8 1.0 HD2 B:PRO27 2.7 28.5 1.0 O B:HOH2027 2.7 40.9 1.0 HB3 B:ASP28 2.8 35.6 1.0 CG B:ASP28 2.9 47.3 1.0 HA B:ALA26 2.9 39.9 1.0 OD1 B:ASP28 3.0 43.7 1.0 N B:ASP28 3.0 24.0 1.0 HG2 B:PRO27 3.1 34.4 1.0 CB B:ASP28 3.2 29.6 1.0 CD B:PRO27 3.3 23.7 1.0 OD2 B:ASP28 3.4 49.2 1.0 N B:PRO27 3.4 24.0 1.0 C B:ALA26 3.6 30.9 1.0 CG B:PRO27 3.7 28.7 1.0 CA B:ALA26 3.7 33.2 1.0 CA B:ASP28 3.7 27.1 1.0 C B:PRO27 4.0 30.0 1.0 HB2 B:ALA26 4.1 44.0 1.0 HB2 B:ASP28 4.1 35.6 1.0 CA B:PRO27 4.2 27.7 1.0 HD3 B:PRO27 4.2 28.5 1.0 O B:HOH2063 4.3 52.7 1.0 O B:ALA26 4.3 28.7 1.0 O B:HOH2031 4.3 52.4 1.0 HA B:ASP28 4.4 32.5 1.0 HG3 B:PRO27 4.4 34.4 1.0 CB B:ALA26 4.4 36.6 1.0 HE2 B:MET66 4.5 79.8 1.0 O B:HOH2032 4.5 52.2 1.0 CB B:PRO27 4.5 32.0 1.0 H B:MET29 4.7 34.5 1.0 HB2 B:PRO27 4.7 38.4 1.0 C B:ASP28 4.8 26.4 1.0 N B:ALA26 4.8 23.9 1.0 HB3 B:ALA26 4.8 44.0 1.0 O B:GLN25 5.0 30.8 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Human G3BP1 in Complex with Semliki Forest Virus NSP3-25 Comprising Two Fgdf Motives within 5.0Å range: probe atom residue distance (Å) B Occ B:K1144 b:63.0 occ:1.00 H B:ALA84 2.5 27.1 1.0 O A:HOH2077 2.7 51.8 1.0 HH A:TYR56 2.8 84.2 1.0 HE2 A:TYR56 2.9 60.5 1.0 HG2 B:PRO8 3.0 40.6 1.0 HA B:HIS83 3.0 27.6 1.0 O A:HOH2137 3.2 29.9 1.0 HB3 B:HIS83 3.2 30.6 1.0 N B:ALA84 3.3 22.6 1.0 OH A:TYR56 3.4 70.2 1.0 HB1 B:ALA84 3.5 30.1 1.0 CE1 A:TYR56 3.5 50.4 1.0 CA B:HIS83 3.7 23.0 1.0 HD2 B:HIS83 3.8 55.6 1.0 HB3 B:ALA84 3.8 30.1 1.0 CB B:HIS83 3.8 25.5 1.0 CZ A:TYR56 3.9 53.2 1.0 CG B:PRO8 4.0 33.9 1.0 CB B:ALA84 4.0 25.1 1.0 O B:HOH2001 4.0 53.2 1.0 C B:HIS83 4.0 22.9 1.0 HB2 B:PRO8 4.2 34.3 1.0 CD2 B:HIS83 4.3 46.4 1.0 CA B:ALA84 4.3 23.6 1.0 CG B:HIS83 4.3 36.2 1.0 HG3 B:PRO8 4.4 40.6 1.0 HD2 B:PRO8 4.5 37.7 1.0 CB B:PRO8 4.6 28.6 1.0 HB3 A:SER39 4.6 30.3 1.0 CD1 A:TYR56 4.6 41.5 1.0 O A:HOH2060 4.7 58.6 1.0 HB2 B:HIS83 4.7 30.6 1.0 HD2 A:TYR56 4.8 49.9 1.0 O B:ALA84 4.8 28.8 1.0 HB3 B:PRO8 4.8 34.3 1.0 CD B:PRO8 4.8 31.4 1.0 O B:ALA82 4.8 24.3 1.0 HB2 B:ALA84 4.9 30.1 1.0 HA B:ALA84 5.0 28.3 1.0

T.Schulte, L.Liu, M.D.Panas, B.Thaa, N.Dickson, B.Gotte, A.Achour, G.M.Mcinerney. Combined Structural, Biochemical and Cellular Evidence Demonstrates That Both Fgdf Motifs in Alphavirus NSP3 Are Required For Efficient Replication. Open Biol V. 6 2016.
ISSN: ESSN 2046-2441
PubMed: 27383630
DOI: 10.1098/RSOB.160078