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Potassium in PDB 5fkt: Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase

Enzymatic activity of Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase

All present enzymatic activity of Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase:
3.2.1.151;

Protein crystallography data

The structure of Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase, PDB code: 5fkt was solved by M.Attia, J.Stepper, G.J.Davies, H.Brumer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 69.48 / 1.52
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 84.120, 94.290, 105.560, 90.00, 103.20, 90.00
R / Rfree (%) 16.726 / 19.384

Other elements in 5fkt:

The structure of Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase also contains other interesting chemical elements:

Bromine (Br) 2 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase (pdb code 5fkt). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase, PDB code: 5fkt:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 5fkt

Go back to Potassium Binding Sites List in 5fkt
Potassium binding site 1 out of 2 in the Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K1772

b:12.1
occ:1.00
O A:ILE351 2.6 9.4 1.0
O A:TYR329 2.7 9.8 1.0
O A:GLY300 2.8 10.7 1.0
O A:HOH2331 3.3 16.7 1.0
BR A:BR1771 3.5 14.8 1.0
C A:ILE351 3.8 8.7 1.0
C A:GLY300 3.9 10.1 1.0
C A:TYR329 3.9 9.2 1.0
CG2 A:ILE351 4.1 9.1 1.0
CA A:ALA301 4.1 9.5 1.0
CA A:PHE330 4.3 8.3 1.0
N A:ILE351 4.4 8.3 1.0
N A:ALA301 4.4 9.1 1.0
CB A:PHE330 4.5 8.3 1.0
CD2 A:TYR329 4.5 14.2 1.0
N A:PHE330 4.6 9.2 1.0
CA A:ILE351 4.6 8.3 1.0
CB A:ALA301 4.7 11.2 1.0
N A:ALA352 4.8 9.8 1.0
CA A:ALA352 4.8 11.8 1.0
N A:GLY300 4.9 9.5 1.0
CB A:ILE351 5.0 9.2 1.0

Potassium binding site 2 out of 2 in 5fkt

Go back to Potassium Binding Sites List in 5fkt
Potassium binding site 2 out of 2 in the Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Unraveling the First Step of Xyloglucan Degradation By the Soil Saprophyte Cellvibrio Japonicus Through the Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K1771

b:11.8
occ:1.00
O B:ILE351 2.6 10.1 1.0
O B:TYR329 2.7 8.4 1.0
O B:GLY300 2.9 9.6 1.0
BR B:BR1770 3.5 12.8 1.0
O B:HOH2316 3.6 8.0 1.0
C B:ILE351 3.8 9.1 1.0
C B:TYR329 3.9 9.3 1.0
C B:GLY300 3.9 9.5 1.0
CA B:ALA301 4.1 9.4 1.0
CG2 B:ILE351 4.1 9.0 1.0
CA B:PHE330 4.2 8.3 1.0
N B:ALA301 4.4 9.2 1.0
CD2 B:TYR329 4.4 10.7 1.0
CB B:PHE330 4.4 8.7 1.0
N B:ILE351 4.5 7.8 1.0
N B:PHE330 4.5 9.0 1.0
CA B:ILE351 4.7 8.7 1.0
CB B:ALA301 4.7 10.3 1.0
N B:ALA352 4.8 9.6 1.0
CA B:ALA352 4.9 10.7 1.0
N B:GLY300 5.0 10.0 1.0
O B:HOH2317 5.0 14.6 1.0

Reference:

M.Attia, J.Stepper, G.J.Davies, H.Brumer. Functional and Structural Characterization of A Potent GH74 Endo-Xyloglucanase From the Soil Saprophyte Cellvibrio Japonicus Unravels the First Step of Xyloglucan Degradation. Febs J. V. 283 1701 2016.
ISSN: ISSN 1742-464X
PubMed: 26929175
DOI: 10.1111/FEBS.13696
Page generated: Mon Aug 12 13:34:17 2024

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