Potassium in PDB 5ezd: Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1

The structure of Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1, PDB code: 5ezd was solved by Z.Hong, P.A.Andreasen, J.P.Morth, J.K.Jensen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.14 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.650, 75.140, 75.260, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 25.7

The binding sites of Potassium atom in the Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1 (pdb code 5ezd). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1, PDB code: 5ezd:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K401 b:69.0 occ:1.00 O A:HOH524 2.8 80.3 1.0 OE1 A:GLU294 3.1 64.2 1.0 O A:HOH512 3.1 66.3 1.0 N A:GLU293 3.3 50.8 1.0 CA A:ARG292 3.8 51.1 1.0 NE1 A:TRP266 3.8 53.6 1.0 CB A:GLU293 4.0 55.3 1.0 C A:ARG292 4.0 50.0 1.0 CB A:ARG292 4.1 55.6 1.0 CD A:GLU294 4.2 88.7 1.0 CA A:GLU293 4.2 50.8 1.0 CE2 A:TRP266 4.4 55.5 1.0 OE2 A:GLU294 4.6 0.1 1.0 CZ2 A:TRP266 4.6 65.3 1.0 O A:LEU291 4.6 67.8 1.0 CD1 A:TRP266 4.7 55.1 1.0 CG A:ARG292 4.8 60.8 1.0 CD A:ARG292 5.0 70.5 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of A Hepatocyte Growth Factor Activator Inhibitor-1 (Hai-1) Fragment Covering the Pkd-Like 'Internal' Domain and Kunitz Domain 1 within 5.0Å range: probe atom residue distance (Å) B Occ B:K403 b:68.9 occ:1.00 O B:HOH526 2.9 74.9 1.0 O B:HOH525 3.1 82.0 1.0 OE1 B:GLU294 3.1 66.4 1.0 O B:HOH508 3.1 62.4 1.0 N B:GLU293 3.2 50.9 1.0 NE1 B:TRP266 3.8 52.5 1.0 CA B:ARG292 3.8 53.7 1.0 CB B:GLU293 3.9 57.5 1.0 C B:ARG292 4.0 47.2 1.0 CB B:ARG292 4.1 55.0 1.0 CA B:GLU293 4.2 49.2 1.0 CD B:GLU294 4.2 89.1 1.0 CE2 B:TRP266 4.4 52.4 1.0 CZ2 B:TRP266 4.5 62.7 1.0 OE2 B:GLU294 4.6 0.0 1.0 O B:LEU291 4.6 66.5 1.0 CD1 B:TRP266 4.6 53.4 1.0 CG B:ARG292 4.8 57.0 1.0

Z.Hong, L.De Meulemeester, A.Jacobi, J.S.Pedersen, J.P.Morth, P.A.Andreasen, J.K.Jensen. Crystal Structure of A Two-Domain Fragment of Hepatocyte Growth Factor Activator Inhibitor-1: Functional Interactions Between the Kunitz-Type Inhibitor Domain-1 and the Neighboring Polycystic Kidney Disease-Like Domain. J.Biol.Chem. V. 291 14340 2016.
ISSN: ESSN 1083-351X
PubMed: 27189939
DOI: 10.1074/JBC.M115.707240