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Potassium in PDB 5but: Crystal Structure of Inactive Conformation of Ktrab K+ Transporter

Protein crystallography data

The structure of Crystal Structure of Inactive Conformation of Ktrab K+ Transporter, PDB code: 5but was solved by R.S.Vieira-Pires, J.H.Morais-Cabral, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 200.00 / 5.97
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 307.060, 79.410, 205.650, 90.00, 98.10, 90.00
R / Rfree (%) 32.5 / 33.9

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Inactive Conformation of Ktrab K+ Transporter (pdb code 5but). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Crystal Structure of Inactive Conformation of Ktrab K+ Transporter, PDB code: 5but:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in 5but

Go back to Potassium Binding Sites List in 5but
Potassium binding site 1 out of 4 in the Crystal Structure of Inactive Conformation of Ktrab K+ Transporter


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Inactive Conformation of Ktrab K+ Transporter within 5.0Å range:
probe atom residue distance (Å) B Occ
I:K501

b:1.0
occ:1.00
O I:THR61 3.0 1.0 1.0
O I:ALA176 3.1 1.0 1.0
O I:THR390 3.2 1.0 1.0
C I:ALA176 3.3 1.0 1.0
O I:VAL391 3.3 1.0 1.0
C I:VAL391 3.4 1.0 1.0
O I:ALA279 3.4 1.0 1.0
O I:THR278 3.4 1.0 1.0
C I:THR61 3.5 1.0 1.0
C I:ALA279 3.5 1.0 1.0
O I:ASN175 3.5 1.0 1.0
C I:THR278 3.5 1.0 1.0
O I:VAL60 3.5 1.0 1.0
N I:GLY392 3.7 1.0 1.0
CA I:ALA176 3.7 1.0 1.0
N I:GLY280 3.7 1.0 1.0
C I:THR390 3.8 1.0 1.0
C I:ASN175 3.8 1.0 1.0
N I:ALA279 3.8 1.0 1.0
N I:ALA176 3.9 1.0 1.0
CA I:VAL391 3.9 1.0 1.0
N I:GLY177 4.0 1.0 1.0
CA I:GLY280 4.0 1.0 1.0
CA I:GLY392 4.0 1.0 1.0
CA I:THR61 4.1 1.0 1.0
CA I:ALA279 4.1 1.0 1.0
N I:GLY62 4.1 1.0 1.0
CA I:THR278 4.1 1.0 1.0
N I:VAL391 4.1 1.0 1.0
C I:VAL60 4.2 1.0 1.0
CA I:GLY62 4.4 1.0 1.0
CA I:GLY177 4.4 1.0 1.0
N I:THR61 4.4 1.0 1.0
CB I:THR278 4.6 1.0 1.0
CA I:ASN175 4.8 1.0 1.0
CA I:THR390 4.8 1.0 1.0
OG1 I:THR390 4.9 1.0 1.0
CB I:THR390 4.9 1.0 1.0
CB I:ASN175 4.9 1.0 1.0
OG1 I:THR278 5.0 1.0 1.0

Potassium binding site 2 out of 4 in 5but

Go back to Potassium Binding Sites List in 5but
Potassium binding site 2 out of 4 in the Crystal Structure of Inactive Conformation of Ktrab K+ Transporter


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Inactive Conformation of Ktrab K+ Transporter within 5.0Å range:
probe atom residue distance (Å) B Occ
J:K501

b:1.0
occ:1.00
O J:THR390 3.0 1.0 1.0
O J:THR61 3.1 1.0 1.0
O J:VAL60 3.1 1.0 1.0
O J:ALA176 3.2 1.0 1.0
O J:ASN175 3.3 1.0 1.0
C J:THR61 3.4 1.0 1.0
C J:ALA176 3.4 1.0 1.0
O J:THR278 3.5 1.0 1.0
C J:THR390 3.6 1.0 1.0
C J:VAL391 3.7 1.0 1.0
C J:THR278 3.7 1.0 1.0
C J:ASN175 3.7 1.0 1.0
CA J:ALA176 3.7 1.0 1.0
C J:VAL60 3.7 1.0 1.0
O J:VAL391 3.7 1.0 1.0
CA J:THR61 3.7 1.0 1.0
N J:ALA176 3.9 1.0 1.0
O J:ALA279 3.9 1.0 1.0
N J:GLY392 4.0 1.0 1.0
C J:ALA279 4.0 1.0 1.0
N J:THR61 4.0 1.0 1.0
CA J:VAL391 4.0 1.0 1.0
N J:VAL391 4.1 1.0 1.0
N J:GLY62 4.1 1.0 1.0
CA J:THR278 4.2 1.0 1.0
N J:ALA279 4.2 1.0 1.0
N J:GLY177 4.2 1.0 1.0
N J:GLY280 4.3 1.0 1.0
CA J:GLY392 4.4 1.0 1.0
CB J:THR278 4.4 1.0 1.0
CB J:THR390 4.4 1.0 1.0
OG1 J:THR390 4.4 1.0 1.0
CA J:ALA279 4.5 1.0 1.0
CA J:THR390 4.5 1.0 1.0
CA J:GLY62 4.5 1.0 1.0
CA J:GLY280 4.5 1.0 1.0
CA J:ASN175 4.7 1.0 1.0
CA J:GLY177 4.8 1.0 1.0
CB J:ASN175 4.8 1.0 1.0
CA J:VAL60 4.9 1.0 1.0
OG1 J:THR278 4.9 1.0 1.0

Potassium binding site 3 out of 4 in 5but

Go back to Potassium Binding Sites List in 5but
Potassium binding site 3 out of 4 in the Crystal Structure of Inactive Conformation of Ktrab K+ Transporter


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Inactive Conformation of Ktrab K+ Transporter within 5.0Å range:
probe atom residue distance (Å) B Occ
K:K501

b:1.0
occ:1.00
C K:THR278 2.9 1.0 1.0
O K:THR278 2.9 1.0 1.0
O K:THR390 3.0 1.0 1.0
N K:ALA279 3.3 1.0 1.0
C K:ALA279 3.3 1.0 1.0
O K:ASN175 3.4 1.0 1.0
O K:VAL391 3.4 1.0 1.0
O K:ALA279 3.5 1.0 1.0
CA K:THR278 3.5 1.0 1.0
C K:VAL391 3.5 1.0 1.0
O K:THR61 3.6 1.0 1.0
C K:ASN175 3.6 1.0 1.0
CA K:ALA279 3.7 1.0 1.0
N K:GLY280 3.7 1.0 1.0
O K:ALA176 3.7 1.0 1.0
C K:ALA176 3.8 1.0 1.0
O K:VAL60 3.8 1.0 1.0
C K:THR390 3.8 1.0 1.0
N K:ALA176 3.8 1.0 1.0
CA K:VAL391 3.9 1.0 1.0
CB K:THR278 3.9 1.0 1.0
CA K:ALA176 4.0 1.0 1.0
N K:GLY392 4.1 1.0 1.0
C K:THR61 4.1 1.0 1.0
CA K:GLY280 4.2 1.0 1.0
N K:VAL391 4.2 1.0 1.0
N K:GLY177 4.3 1.0 1.0
CA K:ASN175 4.3 1.0 1.0
OG1 K:THR278 4.4 1.0 1.0
CB K:ASN175 4.4 1.0 1.0
CA K:GLY392 4.5 1.0 1.0
C K:VAL60 4.6 1.0 1.0
CA K:THR61 4.6 1.0 1.0
OG1 K:THR390 4.8 1.0 1.0
N K:THR278 4.8 1.0 1.0
CA K:GLY177 4.9 1.0 1.0
N K:GLY62 4.9 1.0 1.0
N K:THR61 5.0 1.0 1.0
CA K:THR390 5.0 1.0 1.0
CB K:THR390 5.0 1.0 1.0

Potassium binding site 4 out of 4 in 5but

Go back to Potassium Binding Sites List in 5but
Potassium binding site 4 out of 4 in the Crystal Structure of Inactive Conformation of Ktrab K+ Transporter


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of Inactive Conformation of Ktrab K+ Transporter within 5.0Å range:
probe atom residue distance (Å) B Occ
L:K501

b:1.0
occ:1.00
O L:VAL60 2.9 1.0 1.0
O L:THR390 3.0 1.0 1.0
O L:ASN175 3.1 1.0 1.0
O L:THR61 3.1 1.0 1.0
O L:ALA176 3.2 1.0 1.0
C L:THR61 3.3 1.0 1.0
C L:ALA176 3.4 1.0 1.0
O L:THR278 3.5 1.0 1.0
C L:VAL60 3.5 1.0 1.0
C L:ASN175 3.6 1.0 1.0
CA L:ALA176 3.6 1.0 1.0
C L:THR390 3.6 1.0 1.0
CA L:THR61 3.6 1.0 1.0
C L:THR278 3.8 1.0 1.0
N L:ALA176 3.8 1.0 1.0
N L:THR61 3.8 1.0 1.0
C L:VAL391 3.9 1.0 1.0
O L:VAL391 4.0 1.0 1.0
O L:ALA279 4.0 1.0 1.0
N L:GLY62 4.1 1.0 1.0
N L:VAL391 4.1 1.0 1.0
C L:ALA279 4.2 1.0 1.0
N L:GLY392 4.2 1.0 1.0
CA L:VAL391 4.2 1.0 1.0
CA L:THR278 4.3 1.0 1.0
OG1 L:THR390 4.3 1.0 1.0
CB L:THR390 4.3 1.0 1.0
N L:GLY177 4.3 1.0 1.0
N L:ALA279 4.3 1.0 1.0
CB L:THR278 4.4 1.0 1.0
CA L:THR390 4.4 1.0 1.0
N L:GLY280 4.5 1.0 1.0
CA L:GLY392 4.6 1.0 1.0
CA L:GLY62 4.6 1.0 1.0
CA L:ALA279 4.6 1.0 1.0
CA L:ASN175 4.6 1.0 1.0
CA L:VAL60 4.7 1.0 1.0
CB L:ASN175 4.7 1.0 1.0
CA L:GLY280 4.8 1.0 1.0
OG1 L:THR278 4.8 1.0 1.0
CA L:GLY177 4.9 1.0 1.0
CB L:VAL60 4.9 1.0 1.0
CG1 L:VAL60 4.9 1.0 1.0

Reference:

A.Szollosi, R.S.Vieira-Pires, C.M.Teixeira-Duarte, R.Rocha, J.H.Morais-Cabral. Dissecting the Molecular Mechanism of Nucleotide-Dependent Activation of the Ktrab K+ Transporter. Plos Biol. V. 14 02356 2016.
ISSN: ESSN 1545-7885
PubMed: 26771197
DOI: 10.1371/JOURNAL.PBIO.1002356
Page generated: Mon Aug 12 13:04:06 2024

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