Atomistry » Potassium » PDB 4zun-5avw » 5a71
Atomistry »
  Potassium »
    PDB 4zun-5avw »
      5a71 »

Potassium in PDB 5a71: Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native

Enzymatic activity of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native

All present enzymatic activity of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native:
3.1.1.3;

Protein crystallography data

The structure of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native, PDB code: 5a71 was solved by B.Stauch, S.J.Fisher, M.Cianci, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 71.04 / 0.91
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 39.670, 48.940, 71.610, 88.74, 97.15, 108.44
R / Rfree (%) 11.2 / 13.2

Other elements in 5a71:

The structure of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native also contains other interesting chemical elements:

Sodium (Na) 5 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native (pdb code 5a71). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native, PDB code: 5a71:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in 5a71

Go back to Potassium Binding Sites List in 5a71
Potassium binding site 1 out of 4 in the Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K1320

b:40.3
occ:1.00
 OD1 A:ASP252 2.8 12.8 1.0
O A:HOH2230 3.1 28.4 1.0
OD2 A:ASP252 3.5 12.8 1.0
CG A:ASP252 3.5 11.8 1.0
CD A:ARG249 3.7 23.1 1.0
NE A:ARG249 4.0 28.8 1.0
O A:HOH2424 4.2 13.9 1.0
O A:HOH2427 4.5 19.0 1.0
CG A:ARG249 4.7 16.8 1.0
CB A:ARG249 4.8 14.2 1.0
CZ A:ARG249 5.0 28.1 1.0

Potassium binding site 2 out of 4 in 5a71

Go back to Potassium Binding Sites List in 5a71
Potassium binding site 2 out of 4 in the Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K1321

b:58.6
occ:1.00
 O A:HOH2099 2.4 31.3 1.0
O A:HOH2100 2.5 40.6 1.0
O A:HOH2106 2.6 29.1 1.0
O A:SER29 3.2 18.6 1.0
OG A:SER29 3.3 26.7 1.0
C A:SER29 3.7 18.5 1.0
CB A:SER29 4.1 21.2 1.0
N A:VAL30 4.2 17.0 1.0
O A:HOH2092 4.3 29.1 1.0
CA A:VAL30 4.4 15.2 1.0
CA A:SER29 4.5 20.6 1.0
O A:HOH2107 4.6 30.0 1.0
O A:HOH2090 4.7 24.4 1.0
O A:HOH2105 4.8 30.9 1.0
C A:VAL30 4.8 15.6 1.0
N A:SER31 4.9 14.7 1.0

Potassium binding site 3 out of 4 in 5a71

Go back to Potassium Binding Sites List in 5a71
Potassium binding site 3 out of 4 in the Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K1322

b:52.5
occ:1.00
 O A:HOH2062 2.6 37.8 1.0
O A:LEU20 2.7 12.6 1.0
O A:ASP17 2.9 14.5 1.0
O A:HOH2061 3.0 21.6 1.0
O A:HOH2029 3.7 32.2 1.0
O A:HOH2070 3.8 71.8 1.0
C A:ASP17 3.9 13.3 1.0
C A:LEU20 3.9 10.6 1.0
CG A:PRO27 4.5 19.1 1.0
CD A:PRO27 4.6 17.6 1.0
CA A:ALA18 4.6 13.8 1.0
CA A:LEU20 4.7 9.3 1.0
CB A:LEU20 4.7 11.0 1.0
N A:LEU20 4.7 10.0 1.0
N A:ALA18 4.7 12.0 1.0
CA A:ASP17 4.8 12.3 1.0
O A:HOH2063 4.9 32.2 1.0
C A:ALA18 4.9 12.1 1.0
CB A:ASP17 4.9 13.4 1.0
N A:THR21 4.9 9.7 1.0
O A:ALA18 5.0 14.0 1.0

Potassium binding site 4 out of 4 in 5a71

Go back to Potassium Binding Sites List in 5a71
Potassium binding site 4 out of 4 in the Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Open and Closed Conformations and Protonation States of Candida Antarctica Lipase B: Atomic Resolution Native within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K1321

b:56.6
occ:1.00
 O B:HOH2376 2.5 37.0 1.0
O B:HOH2298 2.7 45.5 1.0
O B:ASN206 3.0 8.6 1.0
OE1 B:GLN247 3.6 13.3 1.0
O B:HOH2333 3.8 30.7 1.0
C B:ASN206 3.8 7.2 1.0
NE2 B:GLN247 3.9 9.7 1.0
O B:HOH2332 3.9 19.0 1.0
CD B:GLN247 4.2 8.7 1.0
O B:GLY207 4.2 7.4 1.0
O B:HOH2203 4.3 51.9 1.0
CA B:ASN206 4.4 7.8 1.0
C B:GLY207 4.5 6.3 1.0
N B:GLY207 4.8 6.7 1.0
CB B:ASN206 4.8 8.8 1.0
O B:HOH2377 4.9 45.4 1.0

Reference:

B.Stauch, S.J.Fisher, M.Cianci. Open and Closed States of Candida Antarctica Lipase B: Protonation and the Mechanism of Interfacial Activation. J.Lipid Res. V. 56 2348 2015.
ISSN: ISSN 0022-2275
PubMed: 26447231
DOI: 10.1194/JLR.M063388
Page generated: Mon Aug 12 12:51:58 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy