Potassium in PDB 5a1i: The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp.

All present enzymatic activity of The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp.:
2.5.1.6;

The structure of The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp., PDB code: 5a1i was solved by B.Murray, S.V.Antonyuk, A.Marina, S.C.Lu, J.M.Mato, S.S.Hasnain, A.L.Rojas, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	73.37 / 1.09
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	67.972, 94.074, 117.216, 90.00, 90.00, 90.00
R / Rfree (%)	10.4 / 12.4

The structure of The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp. also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Potassium atom in the The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp. (pdb code 5a1i). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp., PDB code: 5a1i:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp. within 5.0Å range: probe atom residue distance (Å) B Occ A:K403 b:11.1 occ:0.70 CE A:MET406 2.5 16.4 0.3 OD1 A:ASP258 2.7 11.3 0.8 O1B A:PPK400 2.8 8.8 0.7 O A:HOH1441 2.8 13.6 1.0 O A:ALA259 2.8 10.9 1.0 O A:HOH1047 3.2 8.6 0.7 O A:HOH1362 3.2 10.1 0.2 O A:HOH1278 3.3 8.8 0.7 CG A:ASP258 3.5 9.7 0.8 OD2 A:ASP258 3.8 11.7 0.8 MG A:MG401 3.8 7.3 0.7 C A:ALA259 3.8 8.5 1.0 O A:HOH1362 3.9 14.6 0.8 PB A:PPK400 3.9 8.4 0.7 N A:ALA259 4.0 8.1 1.0 O2B A:PPK400 4.1 10.4 0.7 SD A:MET406 4.2 18.5 0.3 N A:MET406 4.3 25.6 0.3 CA A:ALA259 4.3 8.2 1.0 N A:SAM405 4.3 7.6 0.5 CB A:ALA259 4.4 9.5 1.0 NH2 A:ARG264 4.4 12.3 1.0 C A:ASP258 4.6 8.4 1.0 CB A:ASP258 4.7 10.3 1.0 O3A A:PPK400 4.8 11.3 0.8 CA A:ASP258 4.9 9.1 1.0 N A:GLY260 5.0 7.8 1.0 CG A:MET406 5.0 22.7 0.3

Potassium binding site 2 out of 2 in the The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of The Structure of Human MAT2A in Complex with Sam, Adenosine, Methionine and Ppnp. within 5.0Å range: probe atom residue distance (Å) B Occ A:K404 b:14.2 occ:0.60 O A:HOH1067 2.7 12.5 0.9 O A:HOH1058 2.9 9.8 0.5 N A:PHE139 3.0 7.2 1.0 N A:GLY278 3.0 9.4 1.0 O A:HOH1067 3.1 12.0 0.1 O A:HOH1062 3.2 11.8 0.7 ND1 A:HIS277 3.5 10.9 1.0 CA A:MET138 3.5 7.4 1.0 CE1 A:HIS277 3.7 11.0 1.0 CA A:HIS277 3.7 9.6 1.0 C A:MET138 3.8 7.4 1.0 CG A:HIS277 3.8 9.9 1.0 O A:PHE139 3.8 9.4 1.0 C A:HIS277 3.9 9.2 1.0 CA A:GLY278 4.0 9.5 1.0 O A:HOH1062 4.0 10.3 0.3 NE2 A:HIS277 4.0 10.7 1.0 CA A:PHE139 4.0 7.4 1.0 CD2 A:HIS277 4.1 10.5 1.0 CB A:MET138 4.1 8.0 1.0 CD1 A:PHE139 4.2 7.6 1.0 CB A:PHE139 4.3 7.7 1.0 CB A:HIS277 4.4 10.1 1.0 C A:PHE139 4.4 7.6 1.0 O A:HOH1058 4.5 9.5 0.6 O A:LEU137 4.5 7.6 1.0 O A:ALA276 4.6 10.9 1.0 N A:GLY279 4.7 8.7 1.0 N A:MET138 4.7 7.2 1.0 CB A:ALA295 4.7 8.6 1.0 CG A:PHE139 4.7 7.3 1.0 C A:GLY278 4.8 8.8 1.0 CG A:MET138 4.8 8.2 1.0 N A:HIS277 4.8 9.6 1.0 O A:MET138 5.0 8.2 1.0

B.Murray, S.V.Antonyuk, A.Marina, S.C.Lu, J.M.Mato, S.S.Hasnain, A.L.Rojas. Crystallography Captures Catalytic Steps in Human Methionine Adenosyltransferase Enzymes. Proc.Natl.Acad.Sci.Usa V. 113 2104 2016.
ISSN: ISSN 0027-8424
PubMed: 26858410
DOI: 10.1073/PNAS.1510959113