Potassium in PDB 4umo: Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin

The structure of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin, PDB code: 4umo was solved by D.Sachyani, J.A.Hirsch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.675 / 3.00
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	151.760, 151.760, 56.091, 90.00, 90.00, 120.00
R / Rfree (%)	21.33 / 24.66

The structure of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Potassium atom in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin (pdb code 4umo). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin, PDB code: 4umo:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ A:K301 b:89.5 occ:1.00 CE2 A:PHE398 3.8 74.7 1.0 NH1 A:ARG507 3.8 74.3 1.0 NE B:ARG507 3.8 56.0 1.0 CG B:ARG511 3.9 52.9 1.0 ND1 B:HIS510 4.0 52.1 1.0 NH2 B:ARG507 4.1 74.5 1.0 NE B:ARG511 4.1 64.8 1.0 CD B:ARG511 4.3 65.2 1.0 CZ B:ARG507 4.4 62.1 1.0 CD A:ARG507 4.4 68.1 1.0 CD2 A:PHE398 4.4 79.8 1.0 CE3 A:TRP392 4.5 53.6 1.0 CD2 A:TRP392 4.6 67.3 1.0 CE1 B:HIS510 4.7 57.5 1.0 CG A:TRP392 4.7 64.7 1.0 CB A:TRP392 4.7 55.0 1.0 CZ A:PHE398 4.7 74.6 1.0 CZ A:ARG507 4.7 71.2 1.0 CG B:ARG507 4.7 54.5 1.0 CB B:ARG507 4.7 51.1 1.0 O B:ARG507 4.8 53.8 1.0 CD B:ARG507 4.8 61.4 1.0 NE A:ARG507 4.9 64.8 1.0

Potassium binding site 2 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ A:K302 b:84.3 occ:1.00 N A:PHE398 3.6 68.6 1.0 CB A:PHE398 3.6 70.7 1.0 O D:SER38 3.7 85.8 1.0 CA D:LEU39 3.9 62.3 1.0 CB A:GLU397 4.0 79.9 1.0 C D:LEU39 4.0 60.6 1.0 O D:LEU39 4.1 51.9 1.0 CA A:PHE398 4.2 69.9 1.0 CB A:TYR395 4.3 60.4 1.0 N A:GLU397 4.3 65.0 1.0 NE2 B:HIS510 4.3 63.1 1.0 C A:GLU397 4.4 64.3 1.0 CA A:GLU397 4.4 64.0 1.0 OE1 A:GLU397 4.5 98.1 1.0 N D:GLY40 4.6 68.7 1.0 C D:SER38 4.7 69.5 1.0 C A:TYR395 4.7 59.1 1.0 CG A:PHE398 4.7 69.1 1.0 N D:LEU39 4.8 64.2 1.0 CA A:TYR395 4.8 60.3 1.0 CB D:LEU39 4.8 70.3 1.0 CG A:GLU397 4.9 77.4 1.0 O A:TYR395 4.9 52.5 1.0 CD2 B:HIS510 4.9 59.5 1.0 CG D:LEU39 4.9 79.7 1.0 N A:ILE396 5.0 83.2 1.0

Potassium binding site 3 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ B:K303 b:82.3 occ:1.00 CE2 B:PHE398 3.6 67.3 1.0 NH1 B:ARG507 3.7 61.3 1.0 NE A:ARG507 3.8 64.8 1.0 CG A:ARG511 3.9 62.9 1.0 ND1 A:HIS510 3.9 51.6 1.0 NH2 A:ARG507 4.1 79.7 1.0 CD2 B:PHE398 4.2 65.3 1.0 CD B:ARG507 4.2 61.4 1.0 NE A:ARG511 4.3 75.6 1.0 CD A:ARG511 4.3 72.7 1.0 CZ A:ARG507 4.4 71.2 1.0 CZ B:PHE398 4.5 72.4 1.0 CZ B:ARG507 4.6 62.1 1.0 CE3 B:TRP392 4.6 58.4 1.0 CG A:ARG507 4.7 63.5 1.0 CE1 A:HIS510 4.7 59.1 1.0 CB B:TRP392 4.7 52.4 1.0 CD2 B:TRP392 4.7 67.4 1.0 CB A:ARG507 4.7 54.8 1.0 CG B:TRP392 4.8 64.3 1.0 NE B:ARG507 4.8 56.0 1.0 CD A:ARG507 4.8 68.1 1.0 O A:ARG507 4.8 67.7 1.0 CG A:HIS510 5.0 47.4 1.0

Potassium binding site 4 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ C:K305 b:79.4 occ:1.00 N B:PHE398 3.6 49.7 1.0 O C:SER38 3.6 87.0 1.0 CB B:PHE398 3.7 60.7 1.0 CA C:LEU39 3.9 67.3 1.0 C C:LEU39 4.0 66.1 1.0 CB B:GLU397 4.0 94.0 1.0 O C:LEU39 4.1 70.0 1.0 NE2 A:HIS510 4.2 71.0 1.0 CB B:TYR395 4.2 53.0 1.0 CA B:PHE398 4.2 66.4 1.0 OE1 B:GLU397 4.4 0.9 1.0 N B:GLU397 4.4 69.0 1.0 C B:GLU397 4.4 76.5 1.0 CA B:GLU397 4.5 75.4 1.0 N C:GLY40 4.5 72.9 1.0 C C:SER38 4.6 80.0 1.0 N C:LEU39 4.7 75.8 1.0 C B:TYR395 4.7 58.8 1.0 CG B:GLU397 4.8 0.9 1.0 CG B:PHE398 4.8 55.1 1.0 CD2 A:HIS510 4.8 68.9 1.0 CA B:TYR395 4.8 56.0 1.0 CB C:LEU39 4.9 59.5 1.0 CD B:GLU397 4.9 0.4 1.0 O B:TYR395 4.9 56.5 1.0 CG C:LEU39 5.0 69.6 1.0 CD1 B:TYR395 5.0 55.6 1.0

D.Sachyani, M.Dvir, R.Strulovich, G.Tria, W.Tobelaim, A.Peretz, O.Pongs, D.Svergun, B.Attali, J.A.Hirsch. Structural Basis of A KV7.1 Potassium Channel Gating Module: Studies of the Intracellular C-Terminal Domain in Complex with Calmodulin Structure 2014.
ISSN: ESSN 1878-4186
DOI: 10.1016/J.STR.2014.07.016