Potassium in PDB 4krz: Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi

All present enzymatic activity of Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi:
2.7.1.40;

The structure of Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi, PDB code: 4krz was solved by W.Zhong, H.P.Morgan, I.W.Mcnae, P.A.M.Michels, L.A.Fothergill-Gilmore, M.D.Walkinshaw, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.20 / 2.50
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	113.785, 121.415, 97.160, 90.00, 115.74, 90.00
R / Rfree (%)	16.1 / 22.3

The binding sites of Potassium atom in the Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi (pdb code 4krz). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi, PDB code: 4krz:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi within 5.0Å range: probe atom residue distance (Å) B Occ A:K501 b:49.6 occ:1.00 O A:HOH813 2.7 32.9 1.0 OD1 A:ASP84 2.9 27.1 1.0 OD1 A:ASN52 2.9 25.3 1.0 O1 A:GOL502 3.0 41.8 1.0 O A:THR85 3.1 22.6 1.0 OG A:SER54 3.2 26.5 1.0 CG A:ASP84 3.4 25.5 1.0 C1 A:GOL502 3.5 46.5 1.0 NZ A:LYS239 3.5 24.8 1.0 OG A:SER212 3.8 22.0 1.0 O A:ASP84 3.9 21.5 1.0 OD2 A:ASP84 3.9 26.1 1.0 C A:THR85 3.9 23.3 1.0 C2 A:GOL502 3.9 48.0 1.0 CG A:ASN52 3.9 25.7 1.0 NH2 A:ARG50 3.9 18.1 1.0 CB A:SER54 4.2 27.5 1.0 C A:ASP84 4.2 21.9 1.0 CB A:ASP84 4.3 23.2 1.0 N A:SER54 4.4 27.5 1.0 ND2 A:ASN52 4.4 25.2 1.0 CA A:LYS86 4.5 26.1 1.0 N A:LYS86 4.5 24.3 1.0 N A:THR85 4.5 21.4 1.0 O2 A:GOL502 4.7 45.6 1.0 O A:LYS86 4.7 26.2 1.0 CA A:SER54 4.8 28.3 1.0 N A:PHE53 4.9 24.1 1.0 CA A:ASP84 4.9 21.9 1.0 CA A:THR85 4.9 22.4 1.0 C A:LYS86 4.9 26.8 1.0

Potassium binding site 2 out of 2 in the Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Apo Crystal Structure of Pyruvate Kinase (Pyk) From Trypanosoma Cruzi within 5.0Å range: probe atom residue distance (Å) B Occ B:K501 b:44.3 occ:1.00 OD1 B:ASN52 2.7 30.4 1.0 OD1 B:ASP84 2.9 30.2 1.0 OG B:SER54 3.1 36.8 1.0 O B:THR85 3.1 29.7 1.0 CG B:ASP84 3.6 28.3 1.0 NH2 B:ARG50 3.7 23.0 1.0 O B:HOH831 3.8 33.1 1.0 O B:ASP84 3.8 26.3 1.0 CG B:ASN52 3.8 31.1 1.0 C B:THR85 3.9 30.3 1.0 NZ B:LYS239 4.1 20.6 1.0 N B:SER54 4.2 38.1 1.0 CB B:SER54 4.2 39.1 1.0 OD2 B:ASP84 4.2 28.4 1.0 C B:ASP84 4.2 27.6 1.0 CB B:ASP84 4.2 26.8 1.0 OG B:SER212 4.3 27.4 1.0 N B:PHE53 4.4 33.1 1.0 N B:LYS86 4.5 33.4 1.0 CA B:LYS86 4.5 35.2 1.0 ND2 B:ASN52 4.6 32.2 1.0 N B:THR85 4.6 27.9 1.0 CA B:SER54 4.7 40.5 1.0 CA B:ASN52 4.8 30.0 1.0 CZ B:ARG50 4.8 22.7 1.0 CA B:ASP84 4.9 26.0 1.0 CA B:THR85 4.9 29.1 1.0 CB B:ASN52 4.9 29.8 1.0 C B:ASN52 4.9 32.0 1.0 C B:PHE53 5.0 36.8 1.0

H.P.Morgan, W.Zhong, I.W.Mcnae, P.A.M.Michels, L.A.Fothergill-Gilmore, M.D.Walkinshaw. Structures of Pyruvate Kinases Display Evolutionarily Divergent Allosteric Strategies. R Soc Open Sci. 2014.
ISSN: ESSN 2054-5703
DOI: 10.1098/RSOS.140120