Potassium in PDB 4ki2: Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna

The structure of Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna, PDB code: 4ki2 was solved by A.Feklistov, Darst S.A., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.53 / 3.30
Space group	P 62 2 2
Cell size a, b, c (Å), α, β, γ (°)	118.110, 118.110, 200.845, 90.00, 90.00, 120.00
R / Rfree (%)	21.2 / 26.2

The binding sites of Potassium atom in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna (pdb code 4ki2). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 5 binding sites of Potassium where determined in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna, PDB code: 4ki2:
Jump to Potassium binding site number: 1; 2; 3; 4; 5;

Potassium binding site 1 out of 5 in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ D:K101 b:51.1 occ:0.50 O6 D:DG11 2.7 62.7 1.0 O6 D:DG10 3.1 91.8 1.0 K D:K102 3.2 80.9 0.5 C6 D:DG11 3.6 64.2 1.0 N1 D:DG11 3.8 53.4 1.0 C6 D:DG10 4.0 83.1 1.0 N1 D:DG10 4.4 73.7 1.0 C5 D:DG11 4.9 73.0 1.0

Potassium binding site 2 out of 5 in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ D:K102 b:80.9 occ:0.50 O6 D:DG10 2.7 91.8 1.0 O6 D:DG9 3.0 85.0 1.0 K D:K101 3.2 51.1 0.5 K D:K103 3.6 0.5 0.5 C6 D:DG10 3.6 83.1 1.0 C6 D:DG9 3.8 79.0 1.0 N1 D:DG10 3.9 73.7 1.0 N1 D:DG9 4.2 84.3 1.0 O6 D:DG11 4.8 62.7 1.0 C5 D:DG10 4.9 65.2 1.0 C5 D:DG9 5.0 80.0 1.0

Potassium binding site 3 out of 5 in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ D:K103 b:0.5 occ:0.50 O6 D:DG9 2.6 85.0 1.0 O6 G:DG11 2.9 0.7 1.0 K G:K101 3.5 0.5 0.5 C6 D:DG9 3.5 79.0 1.0 K D:K102 3.6 80.9 0.5 C6 G:DG11 3.8 95.1 1.0 N1 D:DG9 3.9 84.3 1.0 N1 G:DG11 4.1 91.2 1.0 C5 D:DG9 4.7 80.0 1.0 C5 G:DG11 4.9 80.1 1.0

Potassium binding site 4 out of 5 in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ G:K101 b:0.5 occ:0.50 O6 G:DG11 2.6 0.7 1.0 O6 G:DG10 2.9 0.5 1.0 K G:K102 3.5 90.0 0.5 K D:K103 3.5 0.5 0.5 C6 G:DG11 3.5 95.1 1.0 C6 G:DG10 3.8 0.7 1.0 N1 G:DG11 3.9 91.2 1.0 N1 G:DG10 4.0 0.8 1.0 C5 G:DG11 4.7 80.1 1.0 C5 G:DG10 5.0 97.4 1.0

Potassium binding site 5 out of 5 in the Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Crystallographic Analysis of An Rna-Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ G:K102 b:90.0 occ:0.50 O6 G:DG9 2.7 0.6 1.0 O6 G:DG10 2.9 0.5 1.0 K G:K101 3.5 0.5 0.5 C6 G:DG9 3.6 0.0 1.0 C6 G:DG10 3.7 0.7 1.0 N1 G:DG10 3.9 0.8 1.0 N1 G:DG9 4.0 0.3 1.0 C5 G:DG9 4.8 0.5 1.0 C5 G:DG10 5.0 97.4 1.0

A.Feklistov, S.A.Darst. Crystallographic Analysis of An Rna Polymerase Sigma-Subunit Fragment Complexed with -10 Promoter Element Ssdna: Quadruplex Formation As A Possible Tool For Engineering Crystal Contacts in Protein-Ssdna Complexes. Acta Crystallogr.,Sect.F V. 69 950 2013.
ISSN: ISSN 1744-3091
PubMed: 23989139
DOI: 10.1107/S1744309113020368