Potassium in PDB 4jo0: Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae

The structure of Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae, PDB code: 4jo0 was solved by C.J.Knoot, T.M.Makris, C.M.Wilmot, J.D.Lipscomb, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.66 / 2.17
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	153.158, 153.158, 93.356, 90.00, 90.00, 90.00
R / Rfree (%)	20.1 / 23.3

The structure of Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae (pdb code 4jo0). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae, PDB code: 4jo0:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae within 5.0Å range: probe atom residue distance (Å) B Occ A:K605 b:59.1 occ:1.00 O A:TYR153 2.7 39.3 1.0 O A:SER150 2.7 44.7 1.0 O A:TYR148 2.8 45.6 1.0 O A:HOH908 2.9 58.4 1.0 O A:HOH907 3.3 57.4 1.0 O A:ALA149 3.6 52.6 1.0 C A:ALA149 3.7 43.1 1.0 C A:SER150 3.8 47.3 1.0 C A:TYR153 3.8 42.9 1.0 C A:TYR148 3.9 42.8 1.0 CD2 A:TYR153 4.0 41.0 1.0 N A:SER150 4.0 45.4 1.0 CA A:ALA149 4.1 41.7 1.0 CB A:TYR153 4.2 43.2 1.0 CA A:TYR153 4.4 44.8 1.0 N A:ALA149 4.4 42.0 1.0 CA A:SER150 4.5 43.0 1.0 N A:TYR153 4.6 43.4 1.0 CG A:TYR153 4.6 42.1 1.0 N A:GLU151 4.7 50.0 1.0 CA A:GLU151 4.9 45.3 0.5 CA A:GLU151 4.9 48.5 0.5 N A:ASP154 4.9 47.5 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Cmla, A Diiron Beta-Hydroxylase From Streptomyces Venezuelae within 5.0Å range: probe atom residue distance (Å) B Occ A:K606 b:81.9 occ:1.00 O A:SER366 3.3 71.6 1.0 O A:ARG392 3.5 53.3 1.0 N A:GLY394 3.6 65.8 1.0 O A:GLY394 3.6 72.2 1.0 CA A:GLY394 3.8 69.7 1.0 C A:GLY394 4.2 65.8 1.0 C A:ARG392 4.2 58.5 1.0 N A:GLU368 4.3 56.1 1.0 CB A:ARG392 4.4 50.6 1.0 CA A:ALA367 4.4 58.5 1.0 C A:SER366 4.5 62.1 1.0 C A:ALA367 4.6 57.0 1.0 C A:PHE393 4.6 62.3 1.0 CB A:GLU368 4.7 64.2 1.0 N A:PHE393 4.8 56.9 1.0 CA A:PHE393 4.9 61.8 1.0 CG A:GLU368 4.9 74.9 1.0 CA A:ARG392 5.0 53.2 1.0 N A:ALA367 5.0 66.5 1.0

T.M.Makris, C.J.Knoot, C.M.Wilmot, J.D.Lipscomb. Structure of A Dinuclear Iron Cluster-Containing Beta-Hydroxylase Active in Antibiotic Biosynthesis. Biochemistry V. 52 6662 2013.
ISSN: ISSN 0006-2960
PubMed: 23980641
DOI: 10.1021/BI400845B