Potassium in PDB 4ix2: Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
Enzymatic activity of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
All present enzymatic activity of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp:
1.1.1.205;
Protein crystallography data
The structure of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp, PDB code: 4ix2
was solved by
J.Osipiuk,
N.Maltseva,
M.Makowska-Grzyska,
M.Gu,
W.F.Anderson,
A.Joachimiak,
Center For Structural Genomics Of Infectious Diseases(Csgid),
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
38.10 /
2.15
|
Space group
|
C 2 2 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
167.180,
167.158,
93.082,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
20.8 /
25.8
|
Potassium Binding Sites:
The binding sites of Potassium atom in the Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
(pdb code 4ix2). This binding sites where shown within
5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the
Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp, PDB code: 4ix2:
Jump to Potassium binding site number:
1;
2;
3;
4;
Potassium binding site 1 out
of 4 in 4ix2
Go back to
Potassium Binding Sites List in 4ix2
Potassium binding site 1 out
of 4 in the Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 1 of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:K501
b:16.2
occ:1.00
|
O
|
B:CYS307
|
2.7
|
13.8
|
1.0
|
O
|
C:GLU471
|
2.7
|
15.8
|
1.0
|
O
|
B:GLY304
|
2.7
|
14.8
|
1.0
|
O
|
C:HIS473
|
2.9
|
15.6
|
1.0
|
O
|
C:SER472
|
2.9
|
16.3
|
1.0
|
O
|
B:GLY302
|
2.9
|
32.2
|
1.0
|
C
|
C:SER472
|
3.4
|
18.2
|
1.0
|
C
|
C:HIS473
|
3.7
|
14.3
|
1.0
|
C
|
B:CYS307
|
3.7
|
13.2
|
1.0
|
C
|
C:GLU471
|
3.8
|
18.2
|
1.0
|
C
|
B:GLY304
|
3.8
|
7.3
|
1.0
|
CE1
|
C:HIS475
|
3.9
|
16.7
|
1.0
|
ND1
|
C:HIS475
|
4.0
|
19.5
|
1.0
|
C
|
B:PRO303
|
4.0
|
15.7
|
1.0
|
CA
|
C:SER472
|
4.0
|
8.6
|
1.0
|
N
|
C:HIS473
|
4.1
|
16.5
|
1.0
|
N
|
B:GLY304
|
4.1
|
10.5
|
1.0
|
C
|
B:GLY302
|
4.1
|
20.3
|
1.0
|
O
|
B:PRO303
|
4.2
|
14.7
|
1.0
|
CB
|
B:CYS307
|
4.2
|
25.7
|
1.0
|
CA
|
B:CYS307
|
4.3
|
23.1
|
1.0
|
N
|
C:SER472
|
4.3
|
7.0
|
1.0
|
N
|
B:CYS307
|
4.3
|
4.8
|
1.0
|
CA
|
B:PRO303
|
4.3
|
14.4
|
1.0
|
CA
|
C:HIS473
|
4.4
|
20.4
|
1.0
|
N
|
C:VAL474
|
4.5
|
18.1
|
1.0
|
CA
|
B:GLY304
|
4.5
|
7.2
|
1.0
|
CA
|
C:VAL474
|
4.7
|
13.7
|
1.0
|
N
|
B:THR308
|
4.7
|
10.6
|
1.0
|
N
|
B:SER305
|
4.8
|
13.1
|
1.0
|
N
|
B:PRO303
|
4.8
|
17.2
|
1.0
|
O
|
B:SER305
|
4.9
|
22.2
|
1.0
|
C
|
B:SER305
|
4.9
|
18.3
|
1.0
|
CA
|
C:GLU471
|
4.9
|
15.4
|
1.0
|
CA
|
B:SER305
|
4.9
|
9.7
|
1.0
|
|
Potassium binding site 2 out
of 4 in 4ix2
Go back to
Potassium Binding Sites List in 4ix2
Potassium binding site 2 out
of 4 in the Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 2 of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:K503
b:26.0
occ:1.00
|
O
|
C:GLY304
|
2.7
|
36.0
|
1.0
|
O
|
C:CYS307
|
2.7
|
8.9
|
1.0
|
O
|
C:GLY302
|
2.8
|
27.2
|
1.0
|
O
|
C:HOH625
|
3.2
|
4.9
|
1.0
|
C
|
C:CYS307
|
3.6
|
7.7
|
1.0
|
CB
|
C:CYS307
|
3.7
|
7.6
|
1.0
|
C
|
C:GLY304
|
3.8
|
35.1
|
1.0
|
C
|
C:PRO303
|
3.8
|
38.7
|
1.0
|
C
|
C:GLY302
|
3.9
|
15.6
|
1.0
|
N
|
C:GLY304
|
3.9
|
31.7
|
1.0
|
CA
|
C:CYS307
|
4.0
|
12.0
|
1.0
|
O
|
C:PRO303
|
4.0
|
33.5
|
1.0
|
N
|
C:CYS307
|
4.1
|
27.5
|
1.0
|
CA
|
C:PRO303
|
4.1
|
35.4
|
1.0
|
CA
|
C:GLY304
|
4.5
|
31.9
|
1.0
|
N
|
C:PRO303
|
4.5
|
25.9
|
1.0
|
SG
|
C:CYS307
|
4.6
|
30.4
|
1.0
|
N
|
C:THR308
|
4.7
|
4.7
|
1.0
|
N
|
C:SER305
|
4.8
|
23.9
|
1.0
|
O
|
C:SER305
|
4.9
|
8.4
|
1.0
|
C
|
C:SER305
|
4.9
|
9.4
|
1.0
|
CA
|
C:GLY302
|
4.9
|
14.9
|
1.0
|
|
Potassium binding site 3 out
of 4 in 4ix2
Go back to
Potassium Binding Sites List in 4ix2
Potassium binding site 3 out
of 4 in the Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 3 of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:K501
b:18.4
occ:1.00
|
O
|
A:CYS307
|
2.7
|
21.8
|
1.0
|
O
|
D:GLU471
|
2.7
|
19.9
|
1.0
|
O
|
A:GLY304
|
2.7
|
34.6
|
1.0
|
O
|
D:HIS473
|
2.9
|
24.7
|
1.0
|
O
|
D:SER472
|
2.9
|
10.4
|
1.0
|
O
|
A:GLY302
|
3.0
|
20.3
|
1.0
|
C
|
D:SER472
|
3.4
|
14.4
|
1.0
|
O
|
A:HOH608
|
3.5
|
25.4
|
1.0
|
C
|
A:CYS307
|
3.6
|
14.0
|
1.0
|
CE1
|
D:HIS475
|
3.6
|
12.2
|
1.0
|
C
|
D:HIS473
|
3.6
|
24.8
|
1.0
|
C
|
D:GLU471
|
3.8
|
23.3
|
1.0
|
ND1
|
D:HIS475
|
3.8
|
12.3
|
1.0
|
C
|
A:GLY304
|
3.9
|
22.9
|
1.0
|
CA
|
D:SER472
|
4.0
|
6.1
|
1.0
|
CB
|
A:CYS307
|
4.1
|
16.7
|
1.0
|
C
|
A:PRO303
|
4.1
|
10.4
|
1.0
|
N
|
D:HIS473
|
4.1
|
13.4
|
1.0
|
C
|
A:GLY302
|
4.2
|
17.7
|
1.0
|
CA
|
A:CYS307
|
4.2
|
7.5
|
1.0
|
N
|
A:GLY304
|
4.2
|
18.1
|
1.0
|
N
|
A:CYS307
|
4.3
|
12.5
|
1.0
|
O
|
A:PRO303
|
4.3
|
14.6
|
1.0
|
N
|
D:SER472
|
4.3
|
4.9
|
1.0
|
N
|
D:VAL474
|
4.4
|
17.0
|
1.0
|
CA
|
D:HIS473
|
4.4
|
19.4
|
1.0
|
CA
|
A:PRO303
|
4.5
|
14.0
|
1.0
|
CA
|
A:GLY304
|
4.6
|
20.9
|
1.0
|
CA
|
D:VAL474
|
4.6
|
10.3
|
1.0
|
N
|
A:THR308
|
4.6
|
13.5
|
1.0
|
O
|
A:SER305
|
4.7
|
20.9
|
1.0
|
N
|
A:SER305
|
4.8
|
26.7
|
1.0
|
C
|
A:SER305
|
4.9
|
9.7
|
1.0
|
NE2
|
D:HIS475
|
4.9
|
11.8
|
1.0
|
N
|
A:PRO303
|
4.9
|
19.4
|
1.0
|
CA
|
A:SER305
|
4.9
|
22.1
|
1.0
|
CA
|
A:THR308
|
4.9
|
4.5
|
1.0
|
CA
|
D:GLU471
|
5.0
|
20.5
|
1.0
|
|
Potassium binding site 4 out
of 4 in 4ix2
Go back to
Potassium Binding Sites List in 4ix2
Potassium binding site 4 out
of 4 in the Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 4 of Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:K503
b:19.2
occ:1.00
|
O
|
D:GLY304
|
2.7
|
35.8
|
1.0
|
O
|
D:CYS307
|
2.8
|
9.0
|
1.0
|
O
|
D:HOH626
|
3.1
|
19.1
|
1.0
|
O
|
D:GLY302
|
3.2
|
20.2
|
1.0
|
C
|
D:CYS307
|
3.6
|
7.8
|
1.0
|
CB
|
D:CYS307
|
3.7
|
18.9
|
1.0
|
C
|
D:PRO303
|
3.8
|
32.7
|
1.0
|
C
|
D:GLY304
|
3.8
|
32.5
|
1.0
|
O
|
D:PRO303
|
4.0
|
33.5
|
1.0
|
N
|
D:GLY304
|
4.0
|
36.6
|
1.0
|
C
|
D:GLY302
|
4.0
|
22.5
|
1.0
|
CA
|
D:CYS307
|
4.0
|
22.3
|
1.0
|
N
|
D:CYS307
|
4.1
|
27.0
|
1.0
|
CA
|
D:PRO303
|
4.1
|
22.4
|
1.0
|
CA
|
D:GLY304
|
4.5
|
26.7
|
1.0
|
N
|
D:PRO303
|
4.5
|
17.9
|
1.0
|
SG
|
D:CYS307
|
4.7
|
29.8
|
1.0
|
N
|
D:THR308
|
4.7
|
8.9
|
1.0
|
N
|
D:SER305
|
4.9
|
28.9
|
1.0
|
O
|
D:SER305
|
4.9
|
6.6
|
1.0
|
C
|
D:SER305
|
4.9
|
8.7
|
1.0
|
|
Reference:
J.Osipiuk,
N.Maltseva,
M.Makowska-Grzyska,
M.Gu,
W.F.Anderson,
A.Joachimiak.
Inosine 5'-Monophosphate Dehydrogenase From Vibrio Cholerae, Deletion Mutant, Complexed with Imp. To Be Published.
Page generated: Mon Aug 12 11:02:35 2024
|