Potassium in PDB 4it1: Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate

The structure of Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate, PDB code: 4it1 was solved by Y.Patskovsky, R.Toro, R.Bhosle, B.Hillerich, R.D.Seidel, E.Washington, A.Scott Glenn, S.Chowdhury, B.Evans, J.Hammonds, W.D.Zencheck, H.J.Imker, N.Al Obaidi, M.Stead, J.Love, J.A.Gerlt, S.C.Almo, Enzyme Functioninitiative (Efi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.40 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	82.321, 147.695, 173.195, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 25

The structure of Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate (pdb code 4it1). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate, PDB code: 4it1:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate within 5.0Å range: probe atom residue distance (Å) B Occ B:K502 b:66.7 occ:1.00 OD1 B:ASN269 2.6 46.5 1.0 O B:HOH689 2.7 71.2 1.0 OD2 B:ASP221 2.8 49.8 1.0 OE2 B:GLU246 2.8 73.3 1.0 OD2 B:ASP247 3.1 45.2 1.0 OD1 B:ASP221 3.1 48.7 1.0 ND2 B:ASN223 3.2 49.0 1.0 CG B:ASP221 3.3 52.4 1.0 CG B:ASN269 3.7 50.5 1.0 CD B:GLU246 3.8 67.6 1.0 CG B:ASP247 4.0 50.5 1.0 CG B:GLU246 4.1 61.9 1.0 CG B:MET270 4.2 51.7 1.0 CB B:ASP247 4.2 45.8 1.0 CG B:ASN223 4.2 46.8 1.0 N B:ASN269 4.3 46.6 1.0 SD B:MET270 4.4 58.8 1.0 CB B:ASN269 4.4 43.9 1.0 NZ B:LYS191 4.6 72.7 1.0 NZ B:LYS193 4.7 59.9 1.0 CB B:ASP221 4.7 47.3 1.0 O B:HOH690 4.7 74.1 1.0 CA B:ASN223 4.8 53.8 1.0 OD1 B:ASN223 4.8 47.2 1.0 ND2 B:ASN269 4.8 58.3 1.0 CA B:ASN269 4.8 45.9 1.0 CD2 B:HIS320 4.9 50.4 1.0 OE1 B:GLU246 4.9 65.2 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Enolase PFL01_3283 (Target Efi-502286) From Pseudomonas Fluorescens PF0-1 with Bound Magnesium, Potassium and Tartrate within 5.0Å range: probe atom residue distance (Å) B Occ C:K502 b:48.6 occ:1.00 OD1 C:ASN269 2.6 42.8 1.0 OE2 C:GLU246 2.7 58.2 1.0 O C:HOH749 2.8 54.8 1.0 OD2 C:ASP221 2.9 38.0 1.0 OD2 C:ASP247 2.9 41.1 1.0 OD1 C:ASP221 3.0 35.9 1.0 CG C:ASP221 3.2 39.9 1.0 ND2 C:ASN223 3.3 47.6 1.0 CD C:GLU246 3.6 53.9 1.0 CG C:GLU246 3.7 48.0 1.0 CG C:ASP247 3.7 46.7 1.0 CG C:ASN269 3.8 49.7 1.0 CB C:ASP247 3.8 40.4 1.0 O C:HOH680 3.8 66.8 1.0 CG C:MET270 4.1 40.4 1.0 SD C:MET270 4.2 42.9 1.0 CG C:ASN223 4.3 45.7 1.0 N C:ASN269 4.4 35.9 1.0 CB C:ASN269 4.5 44.5 1.0 CB C:ASP221 4.6 42.9 1.0 OE1 C:GLU246 4.7 60.9 1.0 CA C:ASN223 4.7 41.3 1.0 NZ C:LYS191 4.8 67.3 1.0 ND2 C:ASN269 4.8 49.6 1.0 NZ C:LYS193 4.8 46.3 1.0 OD1 C:ASN223 4.8 48.7 1.0 OD1 C:ASP247 4.9 38.1 1.0 CA C:ASN269 4.9 40.8 1.0

Y.Patskovsky, R.Toro, R.Bhosle, B.Hillerich, R.D.Seidel, E.Washington, A.Scott Glenn, S.Chowdhury, B.Evans, J.Hammonds, W.D.Zencheck, H.J.Imker, N.Al Obaidi, M.Stead, J.Love, J.A.Gerlt, S.C.Almo. Crystal Structure of Enolase PFL01_3283 From Pseudomonas Fluorescens To Be Published.