Potassium in PDB 4ib1: Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20

All present enzymatic activity of Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20:
2.7.11.11;

The structure of Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20, PDB code: 4ib1 was solved by O.Gerlits, A.Kovalevsky, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.63
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.893, 79.795, 98.510, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 22.3

The binding sites of Potassium atom in the Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20 (pdb code 4ib1). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20, PDB code: 4ib1:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20 within 5.0Å range: probe atom residue distance (Å) B Occ A:K401 b:20.4 occ:1.00 O1B A:ADP403 2.1 17.2 1.0 O1P S:SEP621 2.3 18.7 1.0 O S:HOH703 2.4 27.0 1.0 OD2 A:ASP184 2.4 12.1 1.0 O A:HOH1003 2.4 24.3 1.0 O A:HOH1002 2.5 21.4 1.0 OD1 A:ASP184 2.5 12.5 1.0 CG A:ASP184 2.8 15.9 1.0 PB A:ADP403 3.4 21.8 1.0 P S:SEP621 3.6 21.6 1.0 O3B A:ADP403 3.7 18.5 1.0 O3P S:SEP621 3.8 28.1 1.0 O S:HOH702 4.0 18.9 1.0 CA A:GLY186 4.1 9.4 1.0 K A:K402 4.1 17.6 1.0 NZ A:LYS72 4.2 15.4 1.0 CB A:ASP184 4.3 10.8 1.0 O A:HOH1161 4.3 17.5 0.5 OD2 A:ASP166 4.3 18.8 1.0 O2B A:ADP403 4.4 22.6 1.0 N A:GLY186 4.4 8.7 1.0 O3A A:ADP403 4.5 21.9 1.0 O2P S:SEP621 4.6 20.7 1.0 OG S:SEP621 4.6 19.9 1.0 O2A A:ADP403 4.8 15.3 1.0 O A:HOH1148 4.8 24.9 1.0

Potassium binding site 2 out of 2 in the Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of Camp Dependent Protein Kinase A in Complex with High K+ Concentration, Adp and Phosphorylated Peptide PSP20 within 5.0Å range: probe atom residue distance (Å) B Occ A:K402 b:17.6 occ:1.00 O2A A:ADP403 2.2 15.3 1.0 O3B A:ADP403 2.3 18.5 1.0 O A:HOH1001 2.4 19.3 1.0 OD2 A:ASP184 2.4 12.1 1.0 O S:HOH702 2.5 18.9 1.0 OD1 A:ASN171 2.5 14.2 1.0 O S:HOH701 2.7 21.5 1.0 CG A:ASN171 3.4 14.2 1.0 CG A:ASP184 3.4 15.9 1.0 PB A:ADP403 3.5 21.8 1.0 PA A:ADP403 3.6 17.8 1.0 O1P S:SEP621 3.6 18.7 1.0 ND2 A:ASN171 3.7 16.3 1.0 CB A:ASP184 3.9 10.8 1.0 O1B A:ADP403 3.9 17.2 1.0 O3A A:ADP403 4.0 21.9 1.0 K A:K401 4.1 20.4 1.0 CE A:LYS168 4.2 10.4 1.0 OD2 A:ASP166 4.4 18.8 1.0 NZ A:LYS168 4.4 10.8 1.0 OD1 A:ASP184 4.5 12.5 1.0 O1A A:ADP403 4.5 17.8 1.0 C5' A:ADP403 4.5 12.9 1.0 O2P S:SEP621 4.5 20.7 1.0 O5' A:ADP403 4.6 15.8 1.0 O S:HOH714 4.7 11.4 0.5 O3' A:ADP403 4.7 15.6 1.0 O2B A:ADP403 4.8 22.6 1.0 P S:SEP621 4.8 21.6 1.0 CB A:ASN171 4.8 8.8 1.0

O.Gerlits, A.Das, M.M.Keshwani, S.Taylor, M.J.Waltman, P.Langan, W.T.Heller, A.Kovalevsky. Metal-Free Camp-Dependent Protein Kinase Can Catalyze Phosphoryl Transfer. Biochemistry V. 53 3179 2014.
ISSN: ISSN 0006-2960
PubMed: 24786636
DOI: 10.1021/BI5000965