Potassium in PDB 4fxf: Structure of M2 Pyruvate Kinase in Complex with Phenylalanine

All present enzymatic activity of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine:
2.7.1.40;

The structure of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine, PDB code: 4fxf was solved by M.D.Walkinshaw, H.P.Morgan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	51.67 / 2.55
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	81.600, 139.350, 111.280, 90.00, 90.52, 90.00
R / Rfree (%)	21.2 / 25.4

The structure of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine also contains other interesting chemical elements:

Magnesium

(Mg)

6 atoms

The binding sites of Potassium atom in the Structure of M2 Pyruvate Kinase in Complex with Phenylalanine (pdb code 4fxf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Structure of M2 Pyruvate Kinase in Complex with Phenylalanine, PDB code: 4fxf:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Structure of M2 Pyruvate Kinase in Complex with Phenylalanine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ A:K602 b:38.9 occ:1.00 O A:HOH702 2.7 20.3 1.0 OD2 A:ASP113 2.7 33.0 1.0 O A:THR114 2.9 32.8 1.0 OD1 A:ASN75 2.9 32.7 1.0 OG A:SER77 3.0 32.1 1.0 CG A:ASP113 3.7 32.2 1.0 OG A:SER243 3.7 35.2 1.0 NZ A:LYS270 3.8 30.8 1.0 C A:THR114 3.9 33.1 1.0 CG A:ASN75 4.0 31.6 1.0 O A:HOH701 4.0 30.4 1.0 CB A:SER77 4.1 32.4 1.0 O A:ASP113 4.1 32.4 1.0 NH2 A:ARG73 4.2 30.7 1.0 CA A:LYS115 4.3 33.5 1.0 OE1 A:GLU118 4.4 34.6 1.0 N A:SER77 4.4 32.6 1.0 C A:ASP113 4.4 32.6 1.0 CB A:ASP113 4.4 32.2 1.0 OD1 A:ASP113 4.5 32.3 1.0 N A:LYS115 4.5 33.5 1.0 O A:LYS115 4.5 33.6 1.0 ND2 A:ASN75 4.5 31.9 1.0 N A:THR114 4.7 32.9 1.0 N A:PHE76 4.8 32.1 1.0 C A:LYS115 4.8 33.8 1.0 CA A:SER77 4.8 32.7 1.0 CB A:SER243 4.9 35.4 1.0 CA A:THR114 4.9 33.3 1.0 O1 A:OXL601 5.0 39.5 1.0

Potassium binding site 2 out of 4 in the Structure of M2 Pyruvate Kinase in Complex with Phenylalanine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ D:K602 b:40.3 occ:1.00 O1G D:ATP604 2.5 51.0 1.0 OD2 D:ASP113 2.7 43.6 1.0 O D:HOH749 2.7 33.8 1.0 O D:THR114 2.8 47.7 1.0 OG D:SER77 2.8 45.2 1.0 OD1 D:ASN75 2.8 42.9 1.0 OG D:SER243 3.3 43.4 1.0 CG D:ASP113 3.8 43.5 1.0 C D:THR114 3.9 48.3 1.0 NZ D:LYS270 3.9 40.9 1.0 CG D:ASN75 3.9 42.5 1.0 PG D:ATP604 3.9 51.2 1.0 CB D:SER77 4.0 45.7 1.0 ND2 D:ASN75 4.3 42.1 1.0 O D:ASP113 4.3 45.7 1.0 O D:LYS115 4.4 50.6 1.0 CA D:LYS115 4.4 50.7 1.0 O3B D:ATP604 4.5 50.8 1.0 CB D:SER243 4.5 43.8 1.0 N D:SER77 4.5 46.0 1.0 C D:ASP113 4.5 45.4 1.0 N D:LYS115 4.6 49.9 1.0 CB D:ASP113 4.6 43.7 1.0 OE2 D:GLU118 4.6 58.5 1.0 O2G D:ATP604 4.6 50.5 1.0 OD1 D:ASP113 4.7 43.4 1.0 N D:THR114 4.7 46.3 1.0 OE1 D:GLU118 4.8 57.8 1.0 NH1 D:ARG120 4.8 63.1 1.0 C D:LYS115 4.8 51.1 1.0 CA D:SER77 4.8 46.6 1.0 CA D:THR114 4.9 47.8 1.0 O3G D:ATP604 4.9 51.0 1.0

Potassium binding site 3 out of 4 in the Structure of M2 Pyruvate Kinase in Complex with Phenylalanine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ C:K602 b:36.6 occ:1.00 O C:HOH792 2.7 31.2 1.0 OD2 C:ASP113 2.7 34.1 1.0 OD1 C:ASN75 2.8 33.2 1.0 O C:THR114 2.8 34.4 1.0 OG C:SER77 2.9 33.4 1.0 O C:HOH791 3.1 31.3 1.0 OG C:SER243 3.8 34.4 1.0 CG C:ASP113 3.8 33.4 1.0 NZ C:LYS270 3.8 33.0 1.0 CG C:ASN75 3.8 32.5 1.0 C C:THR114 3.8 34.9 1.0 O C:ASP113 4.1 33.9 1.0 NH2 C:ARG73 4.1 32.6 1.0 CB C:SER77 4.2 33.4 1.0 OE1 C:GLU118 4.2 37.8 1.0 CA C:LYS115 4.3 35.7 1.0 O C:HOH703 4.3 17.3 1.0 ND2 C:ASN75 4.4 32.2 1.0 N C:SER77 4.4 33.5 1.0 C C:ASP113 4.4 34.0 1.0 N C:LYS115 4.4 35.5 1.0 O C:LYS115 4.5 36.0 1.0 CB C:ASP113 4.6 33.3 1.0 OD1 C:ASP113 4.6 33.2 1.0 C C:LYS115 4.7 36.2 1.0 N C:THR114 4.8 34.4 1.0 N C:PHE76 4.8 33.0 1.0 CA C:SER77 4.8 33.7 1.0 CB C:SER243 4.9 34.9 1.0 O4 C:OXL601 4.9 41.7 1.0 CA C:THR114 4.9 34.9 1.0 CB C:ASN75 5.0 32.3 1.0

Potassium binding site 4 out of 4 in the Structure of M2 Pyruvate Kinase in Complex with Phenylalanine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Structure of M2 Pyruvate Kinase in Complex with Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ B:K602 b:40.0 occ:1.00 O3G B:ATP604 2.6 45.2 1.0 OD2 B:ASP113 2.7 39.0 1.0 OD1 B:ASN75 2.8 39.4 1.0 O B:THR114 2.9 41.9 1.0 OG B:SER77 3.1 41.0 1.0 OG B:SER243 3.3 36.3 1.0 O B:HOH703 3.4 46.4 1.0 NZ B:LYS270 3.8 35.8 1.0 CG B:ASP113 3.8 39.1 1.0 CG B:ASN75 3.9 38.8 1.0 C B:THR114 4.0 42.3 1.0 PG B:ATP604 4.1 45.7 1.0 CB B:SER77 4.1 41.1 1.0 O B:ASP113 4.2 40.4 1.0 CB B:SER243 4.4 36.8 1.0 ND2 B:ASN75 4.4 39.0 1.0 O B:LYS115 4.5 43.6 1.0 C B:ASP113 4.5 40.4 1.0 CA B:LYS115 4.6 43.8 1.0 NH2 B:ARG73 4.6 33.6 1.0 O3B B:ATP604 4.6 45.6 1.0 CB B:ASP113 4.6 39.4 1.0 OE1 B:GLU118 4.6 48.0 1.0 N B:SER77 4.6 41.3 1.0 OD1 B:ASP113 4.7 39.5 1.0 N B:LYS115 4.7 43.3 1.0 NH1 B:ARG120 4.7 49.7 1.0 O1G B:ATP604 4.7 45.6 1.0 OE2 B:GLU118 4.8 48.2 1.0 N B:THR114 4.8 41.0 1.0 C B:LYS115 4.9 43.9 1.0 MG B:MG603 4.9 40.0 1.0 O4 B:OXL601 4.9 53.1 1.0 CA B:SER77 5.0 41.7 1.0

H.P.Morgan, F.J.O'reilly, M.A.Wear, J.R.O'neill, L.A.Fothergill-Gilmore, T.Hupp, M.D.Walkinshaw. M2 Pyruvate Kinase Provides A Mechanism For Nutrient Sensing and Regulation of Cell Proliferation. Proc.Natl.Acad.Sci.Usa V. 110 5881 2013.
ISSN: ISSN 0027-8424
PubMed: 23530218
DOI: 10.1073/PNAS.1217157110