Atomistry » Potassium » PDB 4eei-4gx0 » 4eou
Atomistry »
  Potassium »
    PDB 4eei-4gx0 »
      4eou »

Potassium in PDB 4eou: Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site

Enzymatic activity of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site

All present enzymatic activity of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site:
4.2.1.52;

Protein crystallography data

The structure of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site, PDB code: 4eou was solved by B.A.Boughton, R.C.J.Dobson, C.A.Hutton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.10 / 2.30
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 121.172, 121.172, 109.732, 90.00, 90.00, 120.00
R / Rfree (%) 14.3 / 19.8

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site (pdb code 4eou). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site, PDB code: 4eou:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in 4eou

Go back to Potassium Binding Sites List in 4eou
Potassium binding site 1 out of 4 in the Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K301

b:16.7
occ:1.00
 O A:ILE157 2.6 9.7 1.0
O A:VAL154 2.6 16.9 1.0
O A:ALA152 2.7 12.4 1.0
O A:HOH568 2.7 29.3 1.0
O A:HOH514 2.8 25.1 1.0
O A:HOH588 3.2 41.4 1.0
O A:LYS155 3.2 15.2 1.0
C A:ALA152 3.7 13.2 1.0
C A:ILE157 3.7 10.1 1.0
C A:VAL154 3.7 16.6 1.0
C A:LYS155 3.8 16.4 1.0
CA A:LYS155 4.1 18.2 1.0
N A:ILE157 4.1 10.6 1.0
CA A:ALA152 4.3 12.2 1.0
N A:LYS155 4.4 18.8 1.0
CA A:ILE157 4.5 10.1 1.0
N A:VAL154 4.5 16.3 1.0
C A:LYS153 4.6 16.6 1.0
N A:ASN156 4.6 15.1 1.0
O A:HOH601 4.6 34.9 1.0
N A:ILE158 4.7 10.0 1.0
CA A:ILE158 4.7 10.2 1.0
N A:LYS153 4.7 13.7 1.0
O A:HOH586 4.7 43.2 1.0
CA A:VAL154 4.8 15.5 1.0
CD1 A:PHE181 4.9 10.2 1.0
CB A:ILE157 4.9 9.6 1.0
CA A:LYS153 4.9 15.7 1.0
O A:LYS153 4.9 17.7 1.0

Potassium binding site 2 out of 4 in 4eou

Go back to Potassium Binding Sites List in 4eou
Potassium binding site 2 out of 4 in the Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K303

b:28.4
occ:1.00
 O A:THR126 2.7 17.5 1.0
O A:HOH512 2.8 15.9 1.0
O A:ALA123 2.9 11.8 1.0
O A:HOH557 3.2 36.8 1.0
O A:GLU124 3.2 19.4 1.0
OD1 A:ASN156 3.7 17.4 1.0
C A:GLU124 3.8 18.0 1.0
ND2 A:ASN156 3.8 17.0 1.0
C A:THR126 3.9 16.8 1.0
C A:ALA123 4.0 12.9 1.0
CG A:ASN156 4.0 16.4 1.0
CA A:GLU124 4.0 18.6 1.0
O A:HOH471 4.1 27.0 1.0
N A:THR126 4.3 15.0 1.0
OD1 A:ASP127 4.4 27.1 1.0
N A:GLU124 4.4 15.2 1.0
N A:HIS125 4.7 15.8 1.0
N A:ASP127 4.7 19.2 1.0
CA A:THR126 4.7 14.6 1.0
CA A:ASP127 4.8 20.4 1.0
OE1 A:GLU124 5.0 49.0 1.0

Potassium binding site 3 out of 4 in 4eou

Go back to Potassium Binding Sites List in 4eou
Potassium binding site 3 out of 4 in the Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K301

b:25.4
occ:1.00
 O B:ILE157 2.6 20.0 1.0
O B:VAL154 2.7 20.9 1.0
O B:ALA152 2.7 17.9 1.0
O B:HOH561 3.0 30.6 1.0
O B:LYS155 3.3 19.9 1.0
O B:HOH603 3.4 36.9 1.0
C B:ILE157 3.7 17.2 1.0
C B:ALA152 3.8 18.5 1.0
C B:VAL154 3.8 22.3 1.0
C B:LYS155 3.9 22.6 1.0
N B:ILE157 4.2 18.2 1.0
CA B:LYS155 4.3 25.1 1.0
CA B:ALA152 4.4 16.7 1.0
CA B:ILE157 4.4 16.8 1.0
N B:LYS155 4.4 23.7 1.0
N B:ILE158 4.6 16.6 1.0
C B:LYS153 4.6 23.6 1.0
CA B:ILE158 4.6 16.2 1.0
N B:VAL154 4.6 22.5 1.0
O B:HOH548 4.7 38.4 1.0
N B:LYS153 4.7 18.9 1.0
N B:ASN156 4.8 21.1 1.0
CD1 B:PHE181 4.8 17.6 1.0
CA B:VAL154 4.9 21.4 1.0
CA B:LYS153 4.9 21.5 1.0
CB B:ILE157 4.9 15.6 1.0
O B:LYS153 5.0 23.0 1.0
CB B:ALA152 5.0 15.2 1.0

Potassium binding site 4 out of 4 in 4eou

Go back to Potassium Binding Sites List in 4eou
Potassium binding site 4 out of 4 in the Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of E. Coli Dihydrodipicolinate Synthase with Pyruvate and Succinic Semi-Aldehyde Bound in Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K303

b:34.7
occ:1.00
 O B:THR126 2.6 20.2 1.0
O B:ALA123 2.8 17.3 1.0
O B:HOH500 2.9 26.1 1.0
C B:THR126 3.8 20.5 1.0
O B:GLU124 3.8 22.9 1.0
C B:ALA123 3.8 19.4 1.0
OD1 B:ASN156 3.8 24.4 1.0
CA B:GLU124 3.9 20.9 1.0
C B:GLU124 3.9 22.2 1.0
ND2 B:ASN156 4.0 21.6 1.0
CG B:ASN156 4.1 22.0 1.0
N B:THR126 4.1 20.6 1.0
N B:GLU124 4.3 19.4 1.0
OD1 B:ASP127 4.5 32.5 1.0
CA B:THR126 4.6 20.2 1.0
N B:HIS125 4.7 21.8 1.0
N B:ASP127 4.7 22.6 1.0
CA B:ASP127 4.8 24.5 1.0

Reference:

B.A.Boughton, R.C.Dobson, C.A.Hutton. The Crystal Structure of Dihydrodipicolinate Synthase From Escherichia Coli with Bound Pyruvate and Succinic Acid Semialdehyde: Unambiguous Resolution of the Stereochemistry of the Condensation Product. Proteins V. 80 2117 2012.
ISSN: ISSN 0887-3585
PubMed: 22552955
DOI: 10.1002/PROT.24106
Page generated: Sun Dec 13 23:30:31 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy