Potassium in PDB 4ek1: Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM

All present enzymatic activity of Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM:
1.14.15.1;

The structure of Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM, PDB code: 4ek1 was solved by Y.-T.Lee, D.B.Goodin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.97
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	56.020, 101.530, 72.980, 90.00, 107.39, 90.00
R / Rfree (%)	20.4 / 25.3

The structure of Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM (pdb code 4ek1). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM, PDB code: 4ek1:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:K503 b:21.0 occ:1.00 O A:GLU84 2.6 18.1 1.0 O A:TYR96 2.7 19.1 1.0 O A:GLY93 2.7 21.0 1.0 O A:GLU94 2.7 21.3 1.0 C A:GLU94 3.5 21.7 1.0 C A:GLU84 3.8 18.9 1.0 C A:TYR96 3.8 19.0 1.0 C A:GLY93 3.8 21.7 1.0 CA A:GLU94 3.9 22.8 1.0 N A:TYR96 4.1 20.1 1.0 CG A:GLU84 4.2 24.6 1.0 N A:GLU94 4.3 21.8 1.0 CA A:SER85 4.4 18.7 1.0 CA A:TYR96 4.4 19.2 1.0 N A:ALA95 4.5 20.9 1.0 C A:ALA95 4.5 20.7 1.0 N A:SER85 4.5 18.6 1.0 CB A:TYR96 4.6 19.0 1.0 CB A:SER85 4.7 18.4 1.0 CA A:GLU84 4.8 19.0 1.0 N A:ASP97 4.9 18.1 1.0 CA A:ALA95 4.9 20.9 1.0 O A:ALA95 5.0 20.6 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Electron-Spin Labeled Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ B:K503 b:24.6 occ:1.00 O B:GLU84 2.6 19.4 1.0 O B:TYR96 2.7 19.7 1.0 O B:GLY93 2.8 18.9 1.0 O B:GLU94 2.9 22.9 1.0 OE1 B:GLU84 3.5 36.4 1.0 C B:GLU94 3.5 21.9 1.0 C B:TYR96 3.8 19.6 1.0 C B:GLU84 3.8 19.7 1.0 CA B:GLU94 3.9 22.2 1.0 C B:GLY93 3.9 19.8 1.0 N B:TYR96 4.2 20.2 1.0 CD B:GLU84 4.3 30.7 1.0 N B:GLU94 4.4 20.2 1.0 N B:ALA95 4.5 21.4 1.0 CA B:TYR96 4.5 19.7 1.0 CA B:SER85 4.5 18.7 1.0 OE2 B:GLU84 4.6 34.5 1.0 N B:SER85 4.6 18.8 1.0 C B:ALA95 4.7 20.6 1.0 N B:ASP97 4.7 19.9 1.0 CB B:TYR96 4.8 19.7 1.0 CA B:GLU84 4.9 19.9 1.0 CA B:ASP97 4.9 19.7 1.0 CB B:SER85 4.9 18.5 1.0 CB B:SER102 4.9 18.5 1.0

S.Stoll, Y.T.Lee, M.Zhang, R.F.Wilson, R.D.Britt, D.B.Goodin. Double Electron-Electron Resonance Shows Cytochrome P450CAM Undergoes A Conformational Change in Solution Upon Binding Substrate. Proc.Natl.Acad.Sci.Usa V. 109 12888 2012.
ISSN: ISSN 0027-8424
PubMed: 22826259
DOI: 10.1073/PNAS.1207123109