Potassium in PDB 4a01: Crystal Structure of the H-Translocating Pyrophosphatase

All present enzymatic activity of Crystal Structure of the H-Translocating Pyrophosphatase:
3.6.1.1;

The structure of Crystal Structure of the H-Translocating Pyrophosphatase, PDB code: 4a01 was solved by S.-M.Lin, J.-Y.Tsai, C.-D.Hsiao, C.-L.Chiu, R.-L.Pan, Y.-J.Sun, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.35
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	218.619, 88.241, 159.105, 90.00, 125.53, 90.00
R / Rfree (%)	16.803 / 20.314

The structure of Crystal Structure of the H-Translocating Pyrophosphatase also contains other interesting chemical elements:

Magnesium

(Mg)

10 atoms

The binding sites of Potassium atom in the Crystal Structure of the H-Translocating Pyrophosphatase (pdb code 4a01). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of the H-Translocating Pyrophosphatase, PDB code: 4a01:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of the H-Translocating Pyrophosphatase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the H-Translocating Pyrophosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:K1772 b:62.8 occ:1.00 O A:HOH2214 2.8 34.5 1.0 O A:HOH2236 2.9 27.1 1.0 O A:HOH2137 2.9 43.8 1.0 O A:HOH2216 3.3 36.4 1.0 OD1 A:ASN534 3.4 40.4 1.0 O A:HOH2136 3.4 34.1 1.0 O3 A:2PN1773 3.4 37.6 1.0 O A:GLY533 3.7 33.3 1.0 C A:GLY533 3.9 33.3 1.0 MG A:MG1771 3.9 34.0 1.0 N A:ASN534 4.0 32.5 1.0 O A:HOH2211 4.0 52.0 1.0 CA A:ASN534 4.1 32.1 1.0 CB A:ALA537 4.2 30.4 1.0 O A:HOH2138 4.2 25.3 1.0 OD2 A:ASP287 4.3 34.6 1.0 C A:GLY503 4.3 34.5 1.0 O A:GLY503 4.4 34.5 1.0 CG A:ASN534 4.4 32.0 1.0 O A:HOH2105 4.5 24.9 1.0 O2 A:2PN1773 4.5 30.4 1.0 CA A:GLY503 4.6 33.3 1.0 N A:PRO504 4.6 34.1 1.0 P1 A:2PN1773 4.6 42.7 1.0 CA A:GLY533 4.7 34.0 1.0 CD A:PRO504 4.7 33.5 1.0 OD1 A:ASP530 4.7 38.9 1.0 CB A:ASN534 4.9 30.6 1.0 CA A:PRO504 5.0 34.0 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of the H-Translocating Pyrophosphatase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the H-Translocating Pyrophosphatase within 5.0Å range: probe atom residue distance (Å) B Occ B:K1772 b:60.1 occ:1.00 O B:HOH2167 2.7 34.5 1.0 O B:HOH2181 2.9 28.3 1.0 O B:HOH2169 3.0 41.7 1.0 O B:HOH2098 3.0 46.7 1.0 O3 B:2PN1773 3.0 38.5 1.0 OD1 B:ASN534 3.3 40.9 1.0 O B:HOH2097 3.4 40.9 1.0 MG B:MG1771 3.6 38.8 1.0 O B:GLY533 4.0 41.7 1.0 C B:GLY533 4.0 41.0 1.0 N B:ASN534 4.0 40.2 1.0 CA B:ASN534 4.1 39.2 1.0 P1 B:2PN1773 4.2 47.6 1.0 OD2 B:ASP287 4.3 45.4 1.0 O B:HOH2096 4.3 35.0 1.0 C B:GLY503 4.4 40.8 1.0 O B:GLY503 4.4 40.6 1.0 CG B:ASN534 4.4 38.4 1.0 O2 B:2PN1773 4.4 40.5 1.0 CB B:ALA537 4.4 35.5 1.0 O B:HOH2069 4.4 30.9 1.0 OD1 B:ASP530 4.5 43.4 1.0 N B:PRO504 4.6 40.7 1.0 CA B:GLY503 4.7 40.3 1.0 N1 B:2PN1773 4.7 37.6 1.0 CA B:GLY533 4.8 40.5 1.0 O B:ASP530 4.8 40.3 1.0 CB B:ASN534 4.9 39.4 1.0 OD2 B:ASP507 4.9 39.9 1.0 CB B:ASP507 5.0 39.1 1.0 CD B:PRO504 5.0 39.6 1.0 CA B:PRO504 5.0 40.2 1.0

S.-M.Lin, J.-Y.Tsai, C.-D.Hsiao, C.-L.Chiu, R.-L.Pan, Y.-J.Sun. Crystal Structure of A Membrane Embedded H1-Translocating Pyrophosphatase Nature V. 484 399 2012.
ISSN: ISSN 0028-0836
PubMed: 22456709
DOI: 10.1038/NATURE10963