Potassium in PDB 3zq6: Adp-ALF4 Complex of M. Therm. TRC40

All present enzymatic activity of Adp-ALF4 Complex of M. Therm. TRC40:
3.6.3.16;

The structure of Adp-ALF4 Complex of M. Therm. TRC40, PDB code: 3zq6 was solved by J.Sherrill, M.Mariappan, P.Dominik, R.S.Hegde, R.J.Keenan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.206 / 2.11
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	206.948, 49.768, 147.363, 90.00, 115.04, 90.00
R / Rfree (%)	17.3 / 22.14

The structure of Adp-ALF4 Complex of M. Therm. TRC40 also contains other interesting chemical elements:

Fluorine	(F)	16 atoms
Magnesium	(Mg)	4 atoms
Aluminium	(Al)	4 atoms
Zinc	(Zn)	2 atoms

The binding sites of Potassium atom in the Adp-ALF4 Complex of M. Therm. TRC40 (pdb code 3zq6). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Adp-ALF4 Complex of M. Therm. TRC40, PDB code: 3zq6:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Adp-ALF4 Complex of M. Therm. TRC40


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Adp-ALF4 Complex of M. Therm. TRC40 within 5.0Å range: probe atom residue distance (Å) B Occ A:K404 b:23.1 occ:1.00 O A:ALA53 2.7 15.5 1.0 F4 A:ALF402 2.7 18.6 1.0 OE1 B:GLU238 2.8 18.2 1.0 OG A:SER55 2.8 16.5 1.0 O A:HOH4002 2.9 13.1 1.0 OD2 A:ASP51 3.0 18.1 1.0 O A:HOH2030 3.3 15.1 1.0 O B:HOH2016 3.4 20.1 1.0 O A:HOH4003 3.7 16.8 1.0 CD B:GLU238 3.7 26.4 1.0 CG A:ASP51 3.8 23.0 1.0 C A:ALA53 3.8 19.2 1.0 AL A:ALF402 3.9 16.4 1.0 CB A:SER55 3.9 19.9 1.0 F2 A:ALF402 4.0 18.2 1.0 O A:HOH2034 4.0 26.2 1.0 OE2 B:GLU238 4.0 19.7 1.0 CB A:ALA53 4.0 15.3 1.0 MG A:MG403 4.2 17.5 1.0 OD1 A:ASP51 4.2 13.7 1.0 N A:SER55 4.3 17.9 1.0 C A:HIS54 4.4 19.1 1.0 CE B:LYS22 4.5 12.0 1.0 F1 A:ALF402 4.5 19.7 1.0 CA A:ALA53 4.5 21.8 1.0 CG B:LYS22 4.6 17.2 1.0 CB A:ASP51 4.7 15.5 1.0 CA A:SER55 4.7 17.5 1.0 O A:HIS54 4.7 21.9 1.0 O A:HOH2029 4.7 15.7 1.0 N A:HIS54 4.8 25.9 1.0 CA A:HIS54 4.9 21.8 1.0 O A:HOH2038 4.9 19.6 1.0 CG B:GLU238 5.0 19.0 1.0

Potassium binding site 2 out of 4 in the Adp-ALF4 Complex of M. Therm. TRC40


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Adp-ALF4 Complex of M. Therm. TRC40 within 5.0Å range: probe atom residue distance (Å) B Occ B:K404 b:22.9 occ:1.00 F4 B:ALF402 2.7 20.3 1.0 O B:ALA53 2.7 21.0 1.0 OE1 A:GLU238 2.8 18.6 1.0 OG B:SER55 2.9 21.0 1.0 OD2 B:ASP51 3.0 25.2 1.0 O B:HOH4002 3.1 16.3 1.0 O B:HOH2027 3.2 11.1 1.0 O A:HOH2017 3.3 17.4 1.0 CD A:GLU238 3.7 23.8 1.0 C B:ALA53 3.8 28.5 1.0 CG B:ASP51 3.8 24.1 1.0 O B:HOH4003 3.8 17.0 1.0 AL B:ALF402 3.9 17.1 1.0 CB B:SER55 3.9 22.3 1.0 F2 B:ALF402 3.9 18.6 1.0 OE2 A:GLU238 4.0 26.1 1.0 O A:HOH2151 4.0 26.6 1.0 CB B:ALA53 4.0 18.4 1.0 OD1 B:ASP51 4.3 23.2 1.0 N B:SER55 4.3 25.2 1.0 MG B:MG403 4.3 17.6 1.0 CE A:LYS22 4.4 22.4 1.0 C B:HIS54 4.4 19.2 1.0 CA B:ALA53 4.5 24.1 1.0 F1 B:ALF402 4.5 19.2 1.0 CB B:ASP51 4.7 21.8 1.0 N B:HIS54 4.7 20.9 1.0 CA B:SER55 4.7 24.6 1.0 CG A:LYS22 4.7 16.8 1.0 O A:HOH2103 4.8 19.0 1.0 O B:HIS54 4.8 22.5 1.0 CA B:HIS54 4.8 21.3 1.0 CD A:LYS22 4.9 15.1 1.0 NZ A:LYS22 4.9 16.8 1.0

Potassium binding site 3 out of 4 in the Adp-ALF4 Complex of M. Therm. TRC40


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Adp-ALF4 Complex of M. Therm. TRC40 within 5.0Å range: probe atom residue distance (Å) B Occ C:K404 b:40.1 occ:1.00 F4 C:ALF402 2.7 35.2 1.0 O C:ALA53 2.8 34.5 1.0 OE1 D:GLU238 2.9 40.8 1.0 OD2 C:ASP51 3.0 34.5 1.0 OG C:SER55 3.0 36.1 1.0 O C:HOH4002 3.1 31.6 1.0 O C:HOH2019 3.3 28.6 1.0 O D:HOH2022 3.4 30.5 1.0 O C:HOH4003 3.7 39.2 1.0 CG C:ASP51 3.8 35.4 1.0 CD D:GLU238 3.8 47.1 1.0 F2 C:ALF402 3.9 27.9 1.0 AL C:ALF402 3.9 29.9 1.0 C C:ALA53 3.9 40.9 1.0 CB C:SER55 3.9 33.9 1.0 OE2 D:GLU238 3.9 36.4 1.0 O C:HOH2023 3.9 35.5 1.0 CB C:ALA53 4.1 38.9 1.0 OD1 C:ASP51 4.2 31.7 1.0 MG C:MG403 4.3 31.8 1.0 N C:SER55 4.4 41.7 1.0 CE D:LYS22 4.4 37.3 1.0 C C:HIS54 4.5 42.5 1.0 F1 C:ALF402 4.6 36.3 1.0 CA C:ALA53 4.6 37.5 1.0 O C:HOH2018 4.7 31.0 1.0 O C:HIS54 4.7 39.2 1.0 CB C:ASP51 4.7 22.4 1.0 CA C:SER55 4.7 35.7 1.0 O C:HOH2024 4.8 42.6 1.0 CG D:LYS22 4.8 28.9 1.0 N C:HIS54 4.8 35.9 1.0 CA C:HIS54 4.9 35.1 1.0 NZ D:LYS22 5.0 38.6 1.0 O1B C:ADP401 5.0 27.7 1.0

Potassium binding site 4 out of 4 in the Adp-ALF4 Complex of M. Therm. TRC40


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Adp-ALF4 Complex of M. Therm. TRC40 within 5.0Å range: probe atom residue distance (Å) B Occ D:K404 b:38.1 occ:1.00 O D:ALA53 2.8 39.9 1.0 F4 D:ALF402 2.8 35.8 1.0 OE1 C:GLU238 2.9 37.0 1.0 OG D:SER55 3.0 36.2 1.0 OD2 D:ASP51 3.1 30.4 1.0 O D:HOH4002 3.2 37.9 1.0 O D:HOH2010 3.3 27.6 1.0 O C:HOH2010 3.3 30.1 1.0 O D:HOH4003 3.8 32.8 1.0 CG D:ASP51 3.8 38.9 1.0 CD C:GLU238 3.8 41.0 1.0 CB D:SER55 3.9 42.9 1.0 AL D:ALF402 3.9 33.7 1.0 C D:ALA53 3.9 40.8 1.0 F2 D:ALF402 3.9 42.9 1.0 CB D:ALA53 4.1 29.8 1.0 OE2 C:GLU238 4.1 38.6 1.0 O C:HOH2052 4.1 39.5 1.0 OD1 D:ASP51 4.2 36.9 1.0 N D:SER55 4.3 41.2 1.0 MG D:MG403 4.4 33.1 1.0 C D:HIS54 4.4 41.6 1.0 F1 D:ALF402 4.5 33.1 1.0 CE C:LYS22 4.5 31.6 1.0 CA D:ALA53 4.6 39.1 1.0 CA D:SER55 4.7 39.8 1.0 CG C:LYS22 4.7 31.8 1.0 O D:HIS54 4.7 37.7 1.0 CB D:ASP51 4.7 32.7 1.0 O C:HOH2042 4.8 35.7 1.0 N D:HIS54 4.9 37.0 1.0 CA D:HIS54 4.9 36.6 1.0 O C:HOH2049 4.9 33.2 1.0

J.Sherrill, M.Mariappan, P.Dominik, R.S.Hegde, R.J.Keenan. A Conserved Archaeal Pathway For Tail-Anchored Membrane Protein Insertion. Traffic V. 12 1119 2011.
ISSN: ISSN 1398-9219
PubMed: 21658170
DOI: 10.1111/J.1600-0854.2011.01229.X