Potassium in PDB 3wrh: Crystal Structure of P450CAM

All present enzymatic activity of Crystal Structure of P450CAM:
1.14.15.1;

The structure of Crystal Structure of P450CAM, PDB code: 3wrh was solved by A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, T.Mizushima, H.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.01 / 1.62
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.994, 62.668, 96.038, 90.00, 90.79, 90.00
R / Rfree (%)	17.5 / 20.9

The structure of Crystal Structure of P450CAM also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of P450CAM (pdb code 3wrh). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of P450CAM, PDB code: 3wrh:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:K502 b:7.7 occ:1.00 O A:GLU84 2.6 7.4 1.0 O A:TYR96 2.7 8.1 1.0 O A:GLY93 2.8 7.0 1.0 O A:HOH695 2.8 12.7 1.0 O A:GLU94 2.8 8.3 1.0 C A:GLU94 3.4 7.6 1.0 CA A:GLU94 3.7 7.1 1.0 C A:GLU84 3.8 7.4 1.0 C A:TYR96 3.8 7.7 1.0 C A:GLY93 3.9 7.6 1.0 N A:TYR96 4.1 6.3 1.0 N A:GLU94 4.3 7.9 1.0 CA A:CYS85 4.4 7.1 1.0 CA A:TYR96 4.4 7.0 1.0 N A:ALA95 4.4 7.4 1.0 CG A:GLU84 4.4 22.5 1.0 C A:ALA95 4.5 8.3 1.0 N A:CYS85 4.6 7.3 1.0 CB A:TYR96 4.6 6.7 1.0 CA A:GLU84 4.8 8.1 1.0 N A:ASP97 4.9 7.5 1.0 CB A:CYS85 4.9 7.8 1.0 O A:ALA95 4.9 9.1 1.0 CA A:ALA95 5.0 6.9 1.0 CD A:PRO86 5.0 6.2 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ E:K502 b:11.2 occ:1.00 O E:GLU84 2.5 11.8 1.0 O E:TYR96 2.7 10.9 1.0 O E:GLU94 2.8 12.6 1.0 O E:GLY93 2.8 10.5 1.0 OE1 E:GLU84 3.1 50.3 1.0 C E:GLU94 3.4 10.8 1.0 CA E:GLU94 3.7 10.9 1.0 C E:TYR96 3.8 10.0 1.0 C E:GLU84 3.8 10.7 1.0 C E:GLY93 3.9 10.4 1.0 CD E:GLU84 4.1 39.1 1.0 N E:TYR96 4.1 10.9 1.0 N E:GLU94 4.3 10.6 1.0 CA E:TYR96 4.4 9.4 1.0 CA E:CYS85 4.4 10.3 1.0 N E:ALA95 4.4 10.5 1.0 CG E:GLU84 4.5 28.6 1.0 C E:ALA95 4.5 12.0 1.0 N E:CYS85 4.6 10.6 1.0 CB E:TYR96 4.7 8.9 1.0 N E:ASP97 4.8 9.3 1.0 CA E:GLU84 4.8 12.5 1.0 O E:ALA95 4.8 13.2 1.0 O E:HOH817 4.9 34.2 1.0 CB E:CYS85 5.0 10.7 1.0 CD E:PRO86 5.0 9.5 1.0 CA E:ALA95 5.0 11.6 1.0 CB E:GLU94 5.0 11.4 1.0

A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, Y.Katayama, R.Aminaka, M.Ito, T.Mizushima, H.Shimada. Structure of P450CAM Intermedite To Be Published.