Potassium in PDB 3wlv: Thermostable Urate Oxidase From Bacillus Sp. Tb-90

All present enzymatic activity of Thermostable Urate Oxidase From Bacillus Sp. Tb-90:
1.7.3.3;

The structure of Thermostable Urate Oxidase From Bacillus Sp. Tb-90, PDB code: 3wlv was solved by T.Hibi, Y.Hayashi, T.Itoh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.92 / 1.75
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	133.571, 144.635, 70.794, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 19

The structure of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 also contains other interesting chemical elements:

Chlorine

(Cl)

4 atoms

The binding sites of Potassium atom in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90 (pdb code 3wlv). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90, PDB code: 3wlv:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 within 5.0Å range: probe atom residue distance (Å) B Occ B:K403 b:23.9 occ:0.54 NH1 C:ARG133 2.5 24.5 0.5 O B:SER35 2.9 17.0 1.0 O B:ILE32 2.9 13.9 1.0 CZ C:ARG133 3.5 28.4 0.5 NH2 C:ARG133 3.6 31.3 0.5 C B:SER35 3.7 17.5 1.0 C B:ILE32 3.9 14.4 1.0 O B:PRO33 4.0 16.9 1.0 CA B:PRO36 4.2 24.9 1.0 CA B:PRO33 4.3 19.4 1.0 C B:PRO33 4.3 16.0 1.0 N B:PRO36 4.3 18.1 1.0 N B:SER35 4.4 17.9 1.0 CB B:THR31 4.5 15.1 1.0 N B:PRO33 4.5 17.2 1.0 N B:ILE32 4.7 14.3 1.0 CA B:SER35 4.7 18.4 1.0 OG1 B:THR31 4.7 15.1 1.0 O B:HOH505 4.7 14.3 1.0 NE C:ARG133 4.7 24.6 0.5 O B:HOH656 4.9 33.7 1.0 CA B:ILE32 4.9 14.0 1.0

Potassium binding site 2 out of 2 in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 within 5.0Å range: probe atom residue distance (Å) B Occ C:K402 b:21.7 occ:0.51 NH1 B:ARG133 2.8 22.9 0.6 O C:SER35 2.9 17.9 1.0 O C:ILE32 2.9 13.9 1.0 C C:SER35 3.7 16.2 1.0 CZ B:ARG133 3.7 24.8 0.6 NH2 B:ARG133 3.9 26.4 0.6 C C:ILE32 3.9 15.5 1.0 O C:PRO33 3.9 18.6 1.0 C C:PRO33 4.2 17.9 1.0 CA C:PRO33 4.2 17.3 1.0 N C:PRO36 4.3 19.1 1.0 CA C:PRO36 4.3 21.5 1.0 N C:SER35 4.3 17.2 1.0 CB C:THR31 4.5 14.9 1.0 N C:PRO33 4.5 15.6 1.0 CA C:SER35 4.6 15.9 1.0 N C:ILE32 4.7 15.0 1.0 O C:HOH501 4.7 15.7 1.0 OG1 C:THR31 4.7 15.2 1.0 O C:HOH647 4.9 29.6 1.0 NE B:ARG133 4.9 23.4 0.6 CA C:ILE32 4.9 16.6 1.0

T.Hibi, Y.Hayashi, H.Fukada, T.Itoh, T.Nago, Y.Nishiya. Intersubunit Salt Bridges with A Sulfate Anion Control Subunit Dissociation and Thermal Stabilization of Bacillus Sp. Tb-90 Urate Oxidase Biochemistry 2014.
ISSN: ISSN 0006-2960
PubMed: 24897238
DOI: 10.1021/BI500137B