Potassium in PDB 3unh: Mouse 20S Immunoproteasome
Enzymatic activity of Mouse 20S Immunoproteasome
All present enzymatic activity of Mouse 20S Immunoproteasome:
3.4.25.1;
Protein crystallography data
The structure of Mouse 20S Immunoproteasome, PDB code: 3unh
was solved by
E.Huber,
M.Basler,
R.Schwab,
W.Heinemeyer,
C.Kirk,
M.Groettrup,
M.Groll,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
15.00 /
3.20
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
118.280,
205.220,
161.940,
90.00,
105.70,
90.00
|
R / Rfree (%)
|
23.9 /
25.4
|
Other elements in 3unh:
The structure of Mouse 20S Immunoproteasome also contains other interesting chemical elements:
Potassium Binding Sites:
The binding sites of Potassium atom in the Mouse 20S Immunoproteasome
(pdb code 3unh). This binding sites where shown within
5.0 Angstroms radius around Potassium atom.
In total 10 binding sites of Potassium where determined in the
Mouse 20S Immunoproteasome, PDB code: 3unh:
Jump to Potassium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Potassium binding site 1 out
of 10 in 3unh
Go back to
Potassium Binding Sites List in 3unh
Potassium binding site 1 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 1 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:K249
b:36.0
occ:1.00
|
O
|
C:HOH258
|
2.8
|
39.3
|
1.0
|
O
|
C:GLU98
|
2.8
|
76.7
|
1.0
|
NE
|
C:ARG94
|
3.8
|
67.7
|
1.0
|
C
|
C:GLU98
|
4.1
|
78.0
|
1.0
|
CG
|
C:ARG94
|
4.3
|
67.7
|
1.0
|
NH2
|
C:ARG94
|
4.4
|
67.0
|
1.0
|
CZ
|
C:ARG94
|
4.5
|
66.8
|
1.0
|
CD
|
C:ARG94
|
4.5
|
68.2
|
1.0
|
CG
|
C:PRO100
|
4.5
|
75.0
|
1.0
|
CD
|
C:PRO100
|
4.6
|
75.8
|
1.0
|
NZ
|
J:LYS62
|
4.6
|
54.5
|
1.0
|
N
|
C:PRO100
|
4.7
|
75.8
|
1.0
|
C
|
C:ASP99
|
4.8
|
76.6
|
1.0
|
CG
|
C:GLU98
|
4.9
|
82.8
|
1.0
|
CA
|
C:GLU98
|
4.9
|
79.8
|
1.0
|
N
|
C:ASP99
|
5.0
|
78.2
|
1.0
|
|
Potassium binding site 2 out
of 10 in 3unh
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Potassium Binding Sites List in 3unh
Potassium binding site 2 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 2 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:K234
b:42.3
occ:1.00
|
CG1
|
D:VAL16
|
3.9
|
66.8
|
1.0
|
CD2
|
E:LEU74
|
4.0
|
65.0
|
1.0
|
N
|
D:LEU13
|
4.0
|
71.6
|
1.0
|
CB
|
D:VAL16
|
4.2
|
66.9
|
1.0
|
CB
|
D:ARG12
|
4.2
|
75.3
|
1.0
|
OE1
|
D:GLU17
|
4.2
|
81.7
|
1.0
|
CA
|
D:ARG12
|
4.4
|
75.4
|
1.0
|
CB
|
D:SER151
|
4.5
|
65.0
|
1.0
|
O
|
D:HOH444
|
4.5
|
12.5
|
1.0
|
CG2
|
D:VAL16
|
4.7
|
66.7
|
1.0
|
C
|
D:ARG12
|
4.7
|
72.9
|
1.0
|
O
|
D:LEU13
|
4.8
|
70.0
|
1.0
|
CB
|
D:LEU13
|
4.8
|
68.6
|
1.0
|
CA
|
D:LEU13
|
4.9
|
70.8
|
1.0
|
CD
|
D:GLU17
|
4.9
|
80.2
|
1.0
|
|
Potassium binding site 3 out
of 10 in 3unh
Go back to
Potassium Binding Sites List in 3unh
Potassium binding site 3 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 3 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:K262
b:46.1
occ:1.00
|
O
|
E:HOH271
|
2.9
|
62.2
|
1.0
|
NE2
|
E:GLN13
|
3.4
|
0.9
|
1.0
|
OD1
|
E:ASN1
|
3.7
|
0.3
|
1.0
|
OE1
|
E:GLN13
|
4.0
|
0.6
|
1.0
|
CD1
|
E:TYR3
|
4.1
|
0.8
|
1.0
|
CD
|
E:GLN13
|
4.1
|
0.4
|
1.0
|
OG
|
E:SER11
|
4.2
|
92.4
|
1.0
|
CE1
|
E:TYR3
|
4.4
|
0.3
|
1.0
|
NE2
|
E:HIS17
|
4.6
|
90.7
|
1.0
|
CD
|
E:PRO12
|
4.7
|
91.7
|
1.0
|
CG
|
E:ASN1
|
4.7
|
0.2
|
1.0
|
ND2
|
E:ASN1
|
4.9
|
0.5
|
1.0
|
|
Potassium binding site 4 out
of 10 in 3unh
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Potassium Binding Sites List in 3unh
Potassium binding site 4 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 4 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:K255
b:48.4
occ:1.00
|
O
|
F:HOH295
|
2.7
|
45.2
|
1.0
|
O
|
F:LYS221
|
4.1
|
0.7
|
1.0
|
C
|
F:LYS221
|
4.1
|
0.2
|
1.0
|
CE1
|
F:TYR140
|
4.2
|
0.1
|
1.0
|
N
|
F:GLY222
|
4.4
|
0.4
|
1.0
|
CA
|
F:LYS221
|
4.6
|
0.7
|
1.0
|
N
|
F:ALA142
|
4.6
|
0.1
|
1.0
|
CD1
|
F:TYR140
|
4.6
|
0.7
|
1.0
|
CA
|
F:GLY222
|
4.7
|
95.7
|
1.0
|
CG
|
F:GLU218
|
4.8
|
0.1
|
1.0
|
CB
|
F:ALA142
|
4.8
|
0.4
|
1.0
|
CA
|
F:GLU218
|
4.9
|
0.3
|
1.0
|
|
Potassium binding site 5 out
of 10 in 3unh
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Potassium Binding Sites List in 3unh
Potassium binding site 5 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 5 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
H:K236
b:30.5
occ:1.00
|
OG1
|
H:THR86
|
3.6
|
63.7
|
1.0
|
N
|
H:ALA59
|
3.8
|
71.9
|
1.0
|
O
|
H:THR55
|
3.8
|
65.3
|
1.0
|
CG2
|
H:ILE82
|
3.9
|
65.1
|
1.0
|
CB
|
H:THR86
|
4.0
|
63.1
|
1.0
|
CE
|
H:LYS62
|
4.0
|
79.9
|
1.0
|
CB
|
H:MET58
|
4.0
|
72.0
|
1.0
|
CG2
|
H:THR86
|
4.1
|
62.4
|
1.0
|
CA
|
H:ALA59
|
4.1
|
71.6
|
1.0
|
CB
|
H:ALA59
|
4.2
|
70.8
|
1.0
|
NZ
|
H:LYS62
|
4.3
|
82.0
|
1.0
|
C
|
H:MET58
|
4.3
|
72.5
|
1.0
|
CG2
|
H:THR55
|
4.5
|
62.7
|
1.0
|
CD2
|
H:LEU83
|
4.5
|
61.4
|
1.0
|
O
|
H:ILE82
|
4.7
|
63.1
|
1.0
|
CA
|
H:LEU83
|
4.7
|
61.6
|
1.0
|
CA
|
H:MET58
|
4.8
|
72.2
|
1.0
|
C
|
H:THR55
|
4.8
|
64.5
|
1.0
|
C
|
H:ILE82
|
4.9
|
63.2
|
1.0
|
O
|
H:MET58
|
4.9
|
73.5
|
1.0
|
N
|
H:LEU83
|
4.9
|
62.5
|
1.0
|
|
Potassium binding site 6 out
of 10 in 3unh
Go back to
Potassium Binding Sites List in 3unh
Potassium binding site 6 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 6 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
K:K206
b:43.9
occ:1.00
|
OD1
|
K:ASN38
|
2.1
|
74.3
|
1.0
|
CG
|
K:ASN38
|
3.2
|
71.7
|
1.0
|
ND2
|
K:ASN38
|
3.6
|
68.3
|
1.0
|
CB
|
K:TYR67
|
3.6
|
89.3
|
1.0
|
O
|
K:CYS63
|
3.7
|
72.0
|
1.0
|
CG
|
K:TYR67
|
4.1
|
91.6
|
1.0
|
CA
|
K:ARG64
|
4.1
|
76.8
|
1.0
|
C
|
K:CYS63
|
4.2
|
71.0
|
1.0
|
CZ
|
K:ARG73
|
4.3
|
85.1
|
1.0
|
N
|
K:ARG64
|
4.3
|
73.2
|
1.0
|
NH1
|
K:ARG73
|
4.3
|
87.5
|
1.0
|
CD2
|
K:TYR67
|
4.3
|
92.5
|
1.0
|
NH2
|
K:ARG73
|
4.4
|
83.2
|
1.0
|
CG
|
K:ARG64
|
4.4
|
81.9
|
1.0
|
CB
|
K:ASN38
|
4.5
|
71.0
|
1.0
|
CD1
|
K:TYR67
|
4.8
|
93.4
|
1.0
|
NE
|
K:ARG73
|
4.8
|
86.6
|
1.0
|
CG
|
K:ARG73
|
4.8
|
89.7
|
1.0
|
CB
|
K:ARG64
|
4.8
|
78.7
|
1.0
|
CB
|
K:CYS63
|
5.0
|
64.7
|
1.0
|
|
Potassium binding site 7 out
of 10 in 3unh
Go back to
Potassium Binding Sites List in 3unh
Potassium binding site 7 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 7 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
S:K261
b:42.2
occ:1.00
|
O
|
S:HOH269
|
2.7
|
32.6
|
1.0
|
O
|
S:HOH348
|
2.8
|
52.3
|
1.0
|
NE2
|
S:GLN13
|
2.8
|
96.6
|
1.0
|
CD
|
S:GLN13
|
3.8
|
99.8
|
1.0
|
OE1
|
S:GLN13
|
4.0
|
0.1
|
1.0
|
OG
|
S:SER11
|
4.2
|
86.6
|
1.0
|
CD
|
S:PRO12
|
4.4
|
88.3
|
1.0
|
CD1
|
S:TYR3
|
4.4
|
0.9
|
1.0
|
CE1
|
S:TYR3
|
4.4
|
0.9
|
1.0
|
CG
|
S:PRO12
|
4.5
|
88.5
|
1.0
|
OD1
|
S:ASN1
|
4.6
|
0.3
|
1.0
|
CB
|
S:PRO12
|
4.9
|
88.2
|
1.0
|
|
Potassium binding site 8 out
of 10 in 3unh
Go back to
Potassium Binding Sites List in 3unh
Potassium binding site 8 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 8 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
T:K255
b:40.1
occ:1.00
|
O
|
T:HOH257
|
2.8
|
38.4
|
1.0
|
O
|
T:LYS221
|
4.1
|
83.6
|
1.0
|
C
|
T:LYS221
|
4.1
|
83.6
|
1.0
|
CE1
|
T:TYR140
|
4.2
|
90.6
|
1.0
|
N
|
T:GLY222
|
4.4
|
82.1
|
1.0
|
CD1
|
T:TYR140
|
4.5
|
88.1
|
1.0
|
N
|
T:ALA142
|
4.6
|
95.1
|
1.0
|
CA
|
T:LYS221
|
4.6
|
85.8
|
1.0
|
CA
|
T:GLY222
|
4.7
|
78.4
|
1.0
|
CG
|
T:GLU218
|
4.7
|
88.5
|
1.0
|
CB
|
T:ALA142
|
4.8
|
97.8
|
1.0
|
CA
|
T:GLU218
|
4.9
|
86.1
|
1.0
|
C
|
T:SER141
|
4.9
|
92.9
|
1.0
|
|
Potassium binding site 9 out
of 10 in 3unh
Go back to
Potassium Binding Sites List in 3unh
Potassium binding site 9 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 9 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
Y:K206
b:29.4
occ:1.00
|
O
|
Y:HOH406
|
2.9
|
44.7
|
1.0
|
O
|
Y:ARG30
|
3.1
|
72.6
|
1.0
|
ND2
|
Y:ASN175
|
3.8
|
61.0
|
1.0
|
C
|
Y:ARG30
|
4.1
|
71.8
|
1.0
|
OD1
|
Y:ASN175
|
4.1
|
61.7
|
1.0
|
O
|
Y:MET31
|
4.2
|
65.3
|
1.0
|
CG
|
Y:ASN175
|
4.4
|
60.9
|
1.0
|
C
|
Y:MET31
|
4.4
|
68.9
|
1.0
|
CG
|
Y:ARG30
|
4.5
|
80.8
|
1.0
|
CA
|
Y:ASN32
|
4.6
|
69.1
|
1.0
|
N
|
Y:ASN32
|
4.6
|
69.3
|
1.0
|
CG2
|
Y:VAL173
|
4.6
|
60.2
|
1.0
|
CB
|
Y:ASN32
|
4.6
|
70.6
|
1.0
|
OD1
|
Y:ASN32
|
4.7
|
71.7
|
1.0
|
CA
|
Y:ARG30
|
4.7
|
71.6
|
1.0
|
OG
|
Y:SER189
|
4.9
|
67.3
|
1.0
|
CG
|
Y:ASN32
|
4.9
|
71.7
|
1.0
|
CB
|
Y:SER18
|
4.9
|
57.3
|
1.0
|
N
|
Y:MET31
|
5.0
|
70.7
|
1.0
|
|
Potassium binding site 10 out
of 10 in 3unh
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Potassium Binding Sites List in 3unh
Potassium binding site 10 out
of 10 in the Mouse 20S Immunoproteasome
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 10 of Mouse 20S Immunoproteasome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
b:K202
b:30.7
occ:1.00
|
O
|
a:HOH224
|
2.6
|
28.7
|
1.0
|
NH1
|
a:ARG35
|
4.1
|
72.5
|
1.0
|
CG1
|
b:ILE126
|
4.3
|
57.9
|
1.0
|
O
|
b:PHE124
|
4.3
|
59.5
|
1.0
|
CE1
|
b:PHE124
|
4.4
|
62.6
|
1.0
|
NH1
|
N:ARG165
|
4.4
|
64.0
|
1.0
|
OE1
|
b:GLN122
|
4.5
|
59.4
|
1.0
|
CE2
|
b:TYR135
|
4.5
|
61.6
|
1.0
|
CZ
|
b:PHE124
|
4.6
|
64.1
|
1.0
|
CD1
|
b:ILE126
|
4.7
|
58.7
|
1.0
|
N
|
b:ILE126
|
4.8
|
57.1
|
1.0
|
CD2
|
b:TYR135
|
4.8
|
61.6
|
1.0
|
CD1
|
b:PHE124
|
4.9
|
61.5
|
1.0
|
NH2
|
N:ARG165
|
4.9
|
65.9
|
1.0
|
CA
|
b:THR125
|
4.9
|
57.7
|
1.0
|
CG2
|
b:VAL138
|
4.9
|
63.8
|
1.0
|
|
Reference:
E.M.Huber,
M.Basler,
R.Schwab,
W.Heinemeyer,
C.J.Kirk,
M.Groettrup,
M.Groll.
Immuno- and Constitutive Proteasome Crystal Structures Reveal Differences in Substrate and Inhibitor Specificity. Cell(Cambridge,Mass.) V. 148 727 2012.
ISSN: ISSN 0092-8674
PubMed: 22341445
DOI: 10.1016/J.CELL.2011.12.030
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