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Potassium in PDB 3ugp: Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat)

Protein crystallography data

The structure of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat), PDB code: 3ugp was solved by A.Feklistov, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.22 / 2.70
Space group P 4 2 2
Cell size a, b, c (Å), α, β, γ (°) 86.001, 86.001, 105.862, 90.00, 90.00, 90.00
R / Rfree (%) 19.6 / 23.8

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat) (pdb code 3ugp). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 5 binding sites of Potassium where determined in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat), PDB code: 3ugp:
Jump to Potassium binding site number: 1; 2; 3; 4; 5;

Potassium binding site 1 out of 5 in 3ugp

Go back to Potassium Binding Sites List in 3ugp
Potassium binding site 1 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K12

b:23.1
occ:0.25
O6 B:DG9 3.0 27.0 1.0
O6 C:DG11 3.1 25.7 1.0
K C:K12 3.5 31.0 0.2
K B:K14 3.5 26.7 0.2
C6 C:DG11 3.8 22.1 1.0
C6 B:DG9 3.8 20.4 1.0
N1 B:DG9 3.9 18.8 1.0
N1 C:DG11 3.9 16.6 1.0
C5 C:DG11 5.0 19.3 1.0

Potassium binding site 2 out of 5 in 3ugp

Go back to Potassium Binding Sites List in 3ugp
Potassium binding site 2 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K13

b:32.5
occ:0.25
O6 B:DG11 2.8 18.2 1.0
O6 B:DG10 3.2 29.6 1.0
C6 B:DG11 3.6 19.4 1.0
N1 B:DG11 3.7 15.8 1.0
K B:K14 3.8 26.7 0.2
C6 B:DG10 4.0 22.1 1.0
N1 B:DG10 4.2 18.2 1.0
C5 B:DG11 5.0 17.8 1.0

Potassium binding site 3 out of 5 in 3ugp

Go back to Potassium Binding Sites List in 3ugp
Potassium binding site 3 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K14

b:26.7
occ:0.25
O6 B:DG10 2.9 29.6 1.0
O6 B:DG9 3.1 27.0 1.0
K B:K12 3.5 23.1 0.2
C6 B:DG10 3.7 22.1 1.0
K B:K13 3.8 32.5 0.2
C6 B:DG9 3.8 20.4 1.0
N1 B:DG10 3.9 18.2 1.0
N1 B:DG9 4.0 18.8 1.0
C5 B:DG10 5.0 18.4 1.0

Potassium binding site 4 out of 5 in 3ugp

Go back to Potassium Binding Sites List in 3ugp
Potassium binding site 4 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K12

b:31.0
occ:0.25
O6 C:DG10 3.0 29.7 1.0
O6 C:DG11 3.1 25.7 1.0
K B:K12 3.5 23.1 0.2
C6 C:DG10 3.6 21.2 1.0
N1 C:DG10 3.7 21.1 1.0
C6 C:DG11 3.9 22.1 1.0
K C:K13 3.9 48.0 0.2
N1 C:DG11 4.1 16.6 1.0
C5 C:DG10 4.9 21.5 1.0
C2 C:DG10 5.0 26.6 1.0

Potassium binding site 5 out of 5 in 3ugp

Go back to Potassium Binding Sites List in 3ugp
Potassium binding site 5 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tataat) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K13

b:48.0
occ:0.25
O6 C:DG9 2.9 37.0 1.0
O6 C:DG10 3.4 29.7 1.0
N1 C:DG9 3.6 21.1 1.0
C6 C:DG9 3.6 31.3 1.0
K C:K12 3.9 31.0 0.2
C6 C:DG10 4.1 21.2 1.0
N1 C:DG10 4.3 21.1 1.0
C2 C:DG9 4.9 31.0 1.0
C5 C:DG9 5.0 32.3 1.0

Reference:

A.Feklistov, S.A.Darst. Structural Basis For Promoter-10 Element Recognition By the Bacterial Rna Polymerase Sigma Subunit. Cell(Cambridge,Mass.) V. 147 1257 2011.
ISSN: ISSN 0092-8674
PubMed: 22136875
DOI: 10.1016/J.CELL.2011.10.041
Page generated: Mon Aug 12 09:41:51 2024

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