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Potassium in PDB 3ugo: Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat)

Protein crystallography data

The structure of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat), PDB code: 3ugo was solved by A.Feklistov, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.48 / 2.10
Space group P 4 2 2
Cell size a, b, c (Å), α, β, γ (°) 86.134, 86.134, 111.728, 90.00, 90.00, 90.00
R / Rfree (%) 19.6 / 23.7

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat) (pdb code 3ugo). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 5 binding sites of Potassium where determined in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat), PDB code: 3ugo:
Jump to Potassium binding site number: 1; 2; 3; 4; 5;

Potassium binding site 1 out of 5 in 3ugo

Go back to Potassium Binding Sites List in 3ugo
Potassium binding site 1 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K12

b:30.2
occ:0.25
O6 B:DG9 3.0 23.6 1.0
O6 C:DG11 3.0 25.2 1.0
K C:K12 3.5 27.9 0.2
K B:K14 3.5 25.7 0.2
C6 C:DG11 3.8 27.7 1.0
C6 B:DG9 3.8 23.9 1.0
N1 C:DG11 4.0 26.5 1.0
N1 B:DG9 4.1 21.9 1.0
C5 C:DG11 5.0 21.1 1.0

Potassium binding site 2 out of 5 in 3ugo

Go back to Potassium Binding Sites List in 3ugo
Potassium binding site 2 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K13

b:26.8
occ:0.25
O6 B:DG11 2.7 26.0 1.0
O6 B:DG10 3.1 23.3 1.0
O B:HOH108 3.6 43.4 0.2
C6 B:DG11 3.6 30.2 1.0
K B:K14 3.6 25.7 0.2
N1 B:DG11 3.9 24.1 1.0
C6 B:DG10 3.9 21.1 1.0
N1 B:DG10 4.1 20.7 1.0
C5 B:DG11 4.9 21.6 1.0

Potassium binding site 3 out of 5 in 3ugo

Go back to Potassium Binding Sites List in 3ugo
Potassium binding site 3 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K14

b:25.7
occ:0.25
O6 B:DG10 2.9 23.3 1.0
O6 B:DG9 3.0 23.6 1.0
K B:K12 3.5 30.2 0.2
K B:K13 3.6 26.8 0.2
C6 B:DG10 3.7 21.1 1.0
C6 B:DG9 3.8 23.9 1.0
N1 B:DG10 3.9 20.7 1.0
N1 B:DG9 4.0 21.9 1.0
C5 B:DG9 4.9 20.2 1.0
C5 B:DG10 4.9 20.3 1.0

Potassium binding site 4 out of 5 in 3ugo

Go back to Potassium Binding Sites List in 3ugo
Potassium binding site 4 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K12

b:27.9
occ:0.25
O6 C:DG10 2.9 32.2 1.0
O6 C:DG11 3.1 25.2 1.0
K B:K12 3.5 30.2 0.2
C6 C:DG10 3.7 28.1 1.0
K C:K13 3.8 40.0 0.2
N1 C:DG10 3.9 28.9 1.0
C6 C:DG11 3.9 27.7 1.0
N1 C:DG11 4.2 26.5 1.0
C5 C:DG10 4.9 27.4 1.0

Potassium binding site 5 out of 5 in 3ugo

Go back to Potassium Binding Sites List in 3ugo
Potassium binding site 5 out of 5 in the Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Crystal Structure of Rna-Polymerase Sigma Subunit Domain 2 Complexed with -10 Promoter Element Ssdna Oligo (Tacaat) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K13

b:40.0
occ:0.25
O6 C:DG9 2.8 36.5 1.0
O6 C:DG10 3.3 32.2 1.0
C6 C:DG9 3.6 35.3 1.0
K C:K12 3.8 27.9 0.2
N1 C:DG9 3.8 33.1 1.0
C6 C:DG10 4.1 28.1 1.0
N1 C:DG10 4.3 28.9 1.0
C5 C:DG9 4.9 33.0 1.0

Reference:

A.Feklistov, S.A.Darst. Structural Basis For Promoter-10 Element Recognition By the Bacterial Rna Polymerase Sigma Subunit. Cell(Cambridge,Mass.) V. 147 1257 2011.
ISSN: ISSN 0092-8674
PubMed: 22136875
DOI: 10.1016/J.CELL.2011.10.041
Page generated: Sun Dec 13 23:23:44 2020

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