Potassium in PDB 3ss8: Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+

The structure of Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+, PDB code: 3ss8 was solved by M.R.Ash, M.J.Maher, J.M.Guss, M.Jormakka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.51
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.346, 75.401, 156.494, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 25.2

The structure of Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+ also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Magnesium	(Mg)	2 atoms
Aluminium	(Al)	2 atoms

The binding sites of Potassium atom in the Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+ (pdb code 3ss8). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+, PDB code: 3ss8:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+ within 5.0Å range: probe atom residue distance (Å) B Occ A:K301 b:20.8 occ:1.00 F2 A:ALF303 2.6 24.0 1.0 O2B A:GDP300 2.7 16.6 1.0 O2A A:GDP300 2.8 19.4 1.0 O A:GLY29 2.9 21.0 1.0 O A:TRP31 2.9 21.2 1.0 OD1 A:ASN11 3.2 22.3 1.0 F3 A:ALF303 3.4 23.7 1.0 AL A:ALF303 3.4 24.6 1.0 O3A A:GDP300 3.4 19.5 1.0 PB A:GDP300 3.7 18.5 1.0 PA A:GDP300 3.7 19.3 1.0 O A:HOH272 3.8 7.2 1.0 C A:TRP31 4.0 21.2 1.0 C A:GLY29 4.0 21.1 1.0 C5' A:GDP300 4.0 20.3 1.0 CG2 A:VAL34 4.0 20.6 1.0 CA A:ASN11 4.1 21.0 1.0 CG A:ASN11 4.2 21.7 1.0 CB A:VAL34 4.2 20.9 1.0 F4 A:ALF303 4.2 25.6 1.0 N A:ASN11 4.3 20.8 1.0 O5' A:GDP300 4.3 20.2 1.0 N A:GLY29 4.4 21.7 1.0 O3B A:GDP300 4.4 18.6 1.0 N A:TRP31 4.4 21.0 1.0 N A:VAL34 4.4 20.6 1.0 N A:GLY33 4.6 21.0 1.0 CB A:ASN11 4.6 21.1 1.0 CA A:PRO32 4.6 21.3 1.0 CA A:GLY29 4.6 21.2 1.0 N A:PRO32 4.7 21.3 1.0 F1 A:ALF303 4.8 23.7 1.0 CA A:TRP31 4.9 21.1 1.0 C A:PRO32 4.9 21.2 1.0 MG A:MG302 4.9 17.2 1.0 O A:HOH273 4.9 12.0 1.0 CA A:VAL34 5.0 20.7 1.0 O1B A:GDP300 5.0 19.8 1.0 N A:ASN30 5.0 21.1 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Nfeob From S. Thermophilus Bound to Gdp.ALF4- and K+ within 5.0Å range: probe atom residue distance (Å) B Occ B:K301 b:23.4 occ:1.00 F4 B:ALF303 2.5 20.2 1.0 O2B B:GDP300 2.7 14.6 1.0 O2A B:GDP300 2.8 15.0 1.0 O B:GLY29 2.8 22.7 1.0 O B:TRP31 3.0 23.0 1.0 OD1 B:ASN11 3.1 23.7 1.0 F2 B:ALF303 3.4 19.6 1.0 O3A B:GDP300 3.4 16.7 1.0 AL B:ALF303 3.5 19.9 1.0 PA B:GDP300 3.7 16.3 1.0 PB B:GDP300 3.7 15.5 1.0 O B:HOH272 3.8 13.7 1.0 C B:GLY29 3.9 22.7 1.0 C B:TRP31 4.0 23.0 1.0 CG2 B:VAL34 4.0 21.4 1.0 CA B:ASN11 4.0 20.9 1.0 CG B:ASN11 4.1 22.2 1.0 C5' B:GDP300 4.1 17.8 1.0 CB B:VAL34 4.3 22.2 1.0 N B:ASN11 4.3 20.5 1.0 O5' B:GDP300 4.3 17.8 1.0 N B:TRP31 4.3 22.7 1.0 F1 B:ALF303 4.3 21.7 1.0 N B:GLY29 4.3 22.8 1.0 O3B B:GDP300 4.5 17.2 1.0 CB B:ASN11 4.5 21.1 1.0 N B:VAL34 4.5 22.3 1.0 CA B:GLY29 4.6 22.6 1.0 CA B:PRO32 4.6 23.2 1.0 N B:PRO32 4.7 23.3 1.0 N B:GLY33 4.7 23.1 1.0 CA B:TRP31 4.8 22.8 1.0 F3 B:ALF303 4.9 19.7 1.0 N B:ASN30 4.9 22.5 1.0 O1B B:GDP300 4.9 17.1 1.0 C B:PRO32 5.0 23.3 1.0 MG B:MG302 5.0 19.8 1.0

M.R.Ash, M.J.Maher, J.M.Guss, M.Jormakka. The Initiation of Gtp Hydrolysis By the G-Domain of Feob: Insights From A Transition-State Complex Structure Plos One V. 6 23355 2011.
ISSN: ESSN 1932-6203
PubMed: 21858085
DOI: 10.1371/JOURNAL.PONE.0023355