Potassium in PDB 3srd: Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate.

All present enzymatic activity of Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate.:
2.7.1.40;

The structure of Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate., PDB code: 3srd was solved by H.P.Morgan, F.O'reilly, R.Palmer, I.W.Mcnae, M.W.Nowicki, M.A.Wear, L.A.Fothergill-Gilmore, M.D.Walkinshaw, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.18 / 2.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	95.050, 117.370, 110.460, 90.00, 113.56, 90.00
R / Rfree (%)	25.3 / 27.9

The structure of Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate. also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Potassium atom in the Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate. (pdb code 3srd). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate., PDB code: 3srd:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate. within 5.0Å range: probe atom residue distance (Å) B Occ A:K534 b:56.4 occ:1.00 OD2 A:ASP113 2.5 63.3 1.0 O A:THR114 2.7 64.6 1.0 OD1 A:ASN75 2.7 57.5 1.0 OG A:SER77 3.3 62.2 1.0 CG A:ASP113 3.7 63.0 1.0 OG A:SER243 3.7 59.7 1.0 C A:THR114 3.8 64.9 1.0 CG A:ASN75 3.8 57.7 1.0 NZ A:LYS270 4.0 56.5 1.0 O A:ASP113 4.1 63.4 1.0 CB A:SER77 4.2 62.5 1.0 CA A:LYS115 4.3 66.3 1.0 O A:HOH555 4.3 28.1 1.0 OD1 A:ASP113 4.4 63.8 1.0 N A:LYS115 4.4 65.7 1.0 C A:ASP113 4.4 63.4 1.0 ND2 A:ASN75 4.4 57.8 1.0 O A:LYS115 4.5 66.9 1.0 N A:SER77 4.5 61.5 1.0 NH1 A:ARG73 4.6 55.7 1.0 CB A:ASP113 4.6 62.6 1.0 N A:THR114 4.7 63.9 1.0 OE1 A:GLU118 4.7 72.5 1.0 N A:PHE76 4.8 59.3 1.0 C A:LYS115 4.8 66.8 1.0 CA A:THR114 4.8 64.6 1.0 CB A:SER243 4.9 60.2 1.0 CB A:ASN75 4.9 57.8 1.0 CA A:ASN75 5.0 57.9 1.0

Potassium binding site 2 out of 3 in the Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate. within 5.0Å range: probe atom residue distance (Å) B Occ C:K703 b:78.7 occ:1.00 OD2 C:ASP113 2.9 72.8 1.0 O C:THR114 3.0 74.2 1.0 O C:HOH544 3.0 28.1 1.0 OD1 C:ASN75 3.3 67.7 1.0 OG C:SER77 3.5 72.2 1.0 OG C:SER243 3.6 67.8 1.0 NZ C:LYS270 3.7 65.1 1.0 CG C:ASP113 4.0 72.5 1.0 C C:THR114 4.1 74.7 1.0 CG C:ASN75 4.2 67.9 1.0 O C:LYS115 4.3 75.7 1.0 OE1 C:GLU118 4.3 81.0 1.0 CB C:SER77 4.3 72.7 1.0 CA C:LYS115 4.5 76.0 1.0 O3 C:OXL533 4.5 66.4 1.0 OD1 C:ASP113 4.6 72.9 1.0 CB C:SER243 4.6 68.1 1.0 ND2 C:ASN75 4.6 67.7 1.0 MG C:MG603 4.7 50.1 1.0 N C:LYS115 4.8 75.6 1.0 C C:LYS115 4.8 76.0 1.0 NH1 C:ARG73 4.8 65.0 1.0 O C:ASP113 4.9 73.8 1.0 CE C:LYS270 4.9 65.1 1.0

Potassium binding site 3 out of 3 in the Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Human M2 Pyruvate Kinase in Complex with Fructose 1-6 Bisphosphate and Oxalate. within 5.0Å range: probe atom residue distance (Å) B Occ D:K704 b:64.2 occ:1.00 OD2 D:ASP113 2.8 61.7 1.0 OD1 D:ASN75 2.8 55.5 1.0 O D:THR114 2.8 62.9 1.0 OG D:SER77 3.2 60.4 1.0 OG D:SER243 3.8 60.8 1.0 CG D:ASN75 3.9 55.7 1.0 C D:THR114 3.9 63.1 1.0 CG D:ASP113 3.9 61.2 1.0 NZ D:LYS270 4.1 57.6 1.0 CB D:SER77 4.1 60.7 1.0 CA D:LYS115 4.3 64.5 1.0 O D:LYS115 4.4 65.3 1.0 O D:ASP113 4.4 61.5 1.0 ND2 D:ASN75 4.4 55.9 1.0 N D:LYS115 4.5 63.9 1.0 N D:SER77 4.5 59.5 1.0 OE1 D:GLU118 4.5 70.8 1.0 OD1 D:ASP113 4.6 62.1 1.0 C D:ASP113 4.6 61.4 1.0 NH1 D:ARG73 4.7 54.0 1.0 C D:LYS115 4.7 65.2 1.0 CB D:ASP113 4.8 60.6 1.0 N D:THR114 4.9 62.0 1.0 CB D:SER243 4.9 61.7 1.0 N D:PHE76 4.9 57.2 1.0 CA D:SER77 4.9 60.6 1.0 CA D:THR114 5.0 62.7 1.0 CB D:ASN75 5.0 55.8 1.0

H.P.Morgan, F.O'reilly, R.Palmer, I.W.Mcnae, M.W.Nowicki, M.A.Wear, L.A.Fothergill-Gilmore, M.D.Walkinshaw. Allosetric Regulation of M2 Pyruvate Kinase. To Be Published.