Potassium in PDB 3sph: Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2

The structure of Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2, PDB code: 3sph was solved by S.B.Hansen, X.Tao, R.Mackinnon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.30 / 3.00
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	82.725, 82.725, 185.192, 90.00, 90.00, 90.00
R / Rfree (%)	21.7 / 25.4

The binding sites of Potassium atom in the Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2 (pdb code 3sph). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2, PDB code: 3sph:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:K501 b:89.9 occ:0.25 O A:GLY145 2.7 83.9 1.0 O A:TYR146 2.8 88.7 1.0 K A:K502 2.8 0.3 0.3 C A:TYR146 3.4 88.6 1.0 C A:GLY145 3.8 83.9 1.0 CA A:TYR146 3.9 87.4 1.0 N A:GLY147 4.2 92.4 1.0 N A:TYR146 4.4 86.9 1.0 CA A:GLY147 4.4 93.7 1.0 O A:ILE144 4.8 86.8 1.0

Potassium binding site 2 out of 4 in the Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:K502 b:0.3 occ:0.25 O A:GLY145 2.7 83.9 1.0 O A:ILE144 2.7 86.8 1.0 K A:K501 2.8 89.9 0.2 K A:K503 2.9 89.9 0.2 C A:GLY145 3.5 83.9 1.0 C A:ILE144 3.9 87.1 1.0 CA A:GLY145 4.0 83.6 1.0 N A:TYR146 4.4 86.9 1.0 N A:GLY145 4.4 82.6 1.0 CA A:TYR146 4.7 87.4 1.0 O A:TYR146 4.9 88.7 1.0 C A:TYR146 4.9 88.6 1.0 O A:THR143 4.9 77.7 1.0

Potassium binding site 3 out of 4 in the Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:K503 b:89.9 occ:0.25 O A:THR143 2.6 77.7 1.0 O A:ILE144 2.7 86.8 1.0 K A:K502 2.9 0.3 0.3 K A:K504 3.2 89.8 0.2 C A:ILE144 3.5 87.1 1.0 C A:THR143 3.8 78.3 1.0 CA A:ILE144 4.0 86.5 1.0 N A:ILE144 4.4 86.6 1.0 N A:GLY145 4.4 82.6 1.0 CA A:GLY145 4.8 83.6 1.0 O A:GLY145 4.9 83.9 1.0 CA A:THR143 4.9 78.7 1.0

Potassium binding site 4 out of 4 in the Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Inward Rectifier Potassium Channel KIR2.2 I223L Mutant in Complex with PIP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:K504 b:89.8 occ:0.25 O A:THR143 2.7 77.7 1.0 OG1 A:THR143 2.8 90.7 1.0 K A:K503 3.2 89.9 0.2 CB A:THR143 3.5 91.6 1.0 C A:THR143 3.6 78.3 1.0 CA A:THR143 4.2 78.7 1.0 N A:ILE144 4.6 86.6 1.0 O A:THR142 4.7 77.7 1.0 CG2 A:THR143 4.8 92.1 1.0 CA A:ILE144 4.9 86.5 1.0

S.B.Hansen, X.Tao, R.Mackinnon. Structural Basis of Pip(2) Activation of the Classical Inward Rectifier K(+) Channel KIR2.2. Nature V. 477 495 2011.
ISSN: ISSN 0028-0836
PubMed: 21874019
DOI: 10.1038/NATURE10370