Potassium in PDB 3q8m: Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+

The structure of Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+, PDB code: 3q8m was solved by S.E.Tsutakawa, S.Classen, B.R.Chapados, A.Arvai, D.L.Finger, G.Guenther, C.G.Tomlinson, P.Thompson, A.H.Sarker, B.Shen, P.K.Cooper, J.A.Grasby, J.A.Tainer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	100.93 / 2.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	59.520, 94.940, 104.520, 90.00, 105.06, 90.00
R / Rfree (%)	21.7 / 27.2

The binding sites of Potassium atom in the Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+ (pdb code 3q8m). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+, PDB code: 3q8m:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+ within 5.0Å range: probe atom residue distance (Å) B Occ A:K1102 b:0.7 occ:1.00 O A:ILE241 3.4 66.1 1.0 O A:HOH359 3.4 82.0 1.0 OG A:SER237 3.6 77.4 1.0 OP2 G:DT5 3.7 88.7 1.0 OP1 G:DT5 3.8 70.7 1.0 CB A:SER237 3.8 44.6 1.0 O A:LEU230 4.0 66.3 1.0 O A:LEU229 4.0 68.4 1.0 P G:DT5 4.2 81.7 1.0 O A:ILE238 4.2 89.5 1.0 C A:ILE241 4.4 67.8 1.0 C A:LEU230 4.7 40.1 1.0 CA A:GLY242 4.7 74.7 1.0 O3' G:DC4 4.9 91.4 1.0 N A:GLY242 5.0 62.0 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in Complex with Substrate 5'-Flap Dna and K+ within 5.0Å range: probe atom residue distance (Å) B Occ B:K1101 b:98.2 occ:1.00 O B:HOH348 3.3 59.1 1.0 OP1 D:DT5 3.3 62.3 1.0 O B:ILE238 3.3 78.0 1.0 OG B:SER237 3.5 70.6 1.0 O B:ILE241 3.7 55.4 1.0 CB B:SER237 3.7 41.8 1.0 C B:ILE238 3.9 43.2 1.0 O B:HOH364 4.0 53.1 1.0 OP2 D:DT5 4.1 77.3 1.0 CA B:ARG239 4.2 69.0 1.0 P D:DT5 4.2 57.2 1.0 N B:ARG239 4.3 38.4 1.0 O B:HOH382 4.3 72.0 1.0 C B:SER237 4.4 63.2 1.0 N B:ILE238 4.4 48.7 1.0 O B:HOH396 4.4 46.6 1.0 N B:GLY240 4.6 69.1 1.0 C B:ILE241 4.7 67.3 1.0 CA B:SER237 4.7 42.0 1.0 O B:SER237 4.7 47.2 1.0 O B:LEU229 4.8 61.4 1.0 CA B:ILE238 4.8 56.8 1.0 C B:ARG239 4.8 59.6 1.0

S.E.Tsutakawa, S.Classen, B.R.Chapados, A.S.Arvai, L.D.Finger, G.Guenther, C.G.Tomlinson, P.Thompson, A.H.Sarker, B.Shen, P.K.Cooper, J.A.Grasby, J.A.Tainer. Human Flap Endonuclease Structures, Dna Double-Base Flipping, and A Unified Understanding of the FEN1 Superfamily. Cell(Cambridge,Mass.) V. 145 198 2011.
ISSN: ISSN 0092-8674
PubMed: 21496641
DOI: 10.1016/J.CELL.2011.03.004