Potassium in PDB 3oyz: Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex

All present enzymatic activity of Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex:
2.3.3.9;

The structure of Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex, PDB code: 3oyz was solved by B.R.Howard, C.Bracken, A.Neighbor, G.Thomas, K.K.Lamlenn, H.L.Schubert, F.G.Whitby, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.95
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	154.788, 154.788, 142.144, 90.00, 90.00, 120.00
R / Rfree (%)	20.3 / 23.9

The structure of Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Chlorine	(Cl)	4 atoms

The binding sites of Potassium atom in the Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex (pdb code 3oyz). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex, PDB code: 3oyz:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:K601 b:33.9 occ:1.00 O A:MET173 2.6 34.1 1.0 O A:LYS175 2.6 32.8 1.0 O A:ASN178 2.7 29.4 1.0 O A:HOH483 2.7 51.2 1.0 OE1 A:GLU181 2.7 38.3 1.0 OD1 A:ASN178 2.8 27.9 1.0 OE2 A:GLU181 3.1 55.1 1.0 CD A:GLU181 3.2 52.7 1.0 C A:ASN178 3.5 29.8 1.0 C A:MET173 3.6 33.7 1.0 C A:LYS175 3.7 34.8 1.0 CG A:ASN178 3.8 28.8 1.0 C A:GLY174 4.0 35.0 1.0 N A:LYS175 4.1 34.7 1.0 N A:LEU180 4.2 27.8 1.0 N A:ASN179 4.2 28.9 1.0 O A:GLY174 4.2 35.7 1.0 CA A:ASN179 4.3 30.8 1.0 CA A:ASN178 4.4 29.1 1.0 CA A:GLY174 4.4 33.8 1.0 CA A:PRO176 4.4 33.9 1.0 N A:ASN178 4.4 29.9 1.0 ND2 A:ASN178 4.4 26.7 1.0 N A:GLY174 4.5 33.9 1.0 CA A:MET173 4.5 32.0 1.0 CA A:LYS175 4.5 33.2 1.0 CG A:GLU181 4.5 41.5 1.0 C A:ASN179 4.6 31.9 1.0 K A:K603 4.6 51.8 1.0 N A:GLU181 4.6 27.1 1.0 CB A:ASN178 4.7 25.6 1.0 N A:PRO176 4.7 35.3 1.0 O A:GLU172 4.9 31.5 1.0 C A:PRO176 4.9 34.7 1.0

Potassium binding site 2 out of 3 in the Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:K602 b:37.1 occ:1.00 OG A:SER217 2.7 34.6 1.0 O A:GLY252 2.9 31.9 1.0 OG A:SER221 2.9 38.6 1.0 CL A:CL705 3.1 34.5 1.0 O A:SER217 3.1 28.6 1.0 C A:SER217 3.7 29.2 1.0 C A:GLY252 3.8 31.3 1.0 CB A:SER217 3.8 30.8 1.0 CA A:SER217 3.9 29.7 1.0 CB A:SER221 4.2 29.2 1.0 CA A:GLY252 4.2 33.0 1.0 O A:HOH518 4.3 55.2 1.0 N A:SER221 4.4 30.9 1.0 CB A:ALA227 4.6 26.1 1.0 CA A:SER221 4.8 31.8 1.0 O A:LYS251 4.8 35.1 1.0 CB A:ALA220 4.8 27.1 1.0 N A:MET253 4.8 32.0 1.0 N A:ARG218 4.9 26.8 1.0

Potassium binding site 3 out of 3 in the Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Haloferax Volcanii Malate Synthase Pyruvate/Acetyl-Coa Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:K603 b:51.8 occ:1.00 OD1 A:ASN179 2.7 37.4 1.0 O A:ASN178 2.7 29.4 1.0 O A:PRO176 2.9 30.6 1.0 O A:HOH482 3.3 43.8 1.0 C A:ASN178 3.5 29.8 1.0 CG A:ASN179 3.6 35.3 1.0 C A:PRO176 3.8 34.7 1.0 O A:HOH483 3.9 51.2 1.0 ND2 A:ASN179 4.1 40.4 1.0 O A:HOH561 4.2 51.0 1.0 N A:ASN178 4.2 29.9 1.0 N A:ASN179 4.2 28.9 1.0 CA A:PRO176 4.3 33.9 1.0 CA A:ASN179 4.4 30.8 1.0 CA A:ASN178 4.4 29.1 1.0 CB A:ASN179 4.6 31.1 1.0 K A:K601 4.6 33.9 1.0 C A:THR177 4.7 31.7 1.0 N A:THR177 4.8 31.6 1.0 O A:LYS175 4.9 32.8 1.0 CB A:PRO176 5.0 35.6 1.0

C.D.Bracken, A.M.Neighbor, K.K.Lamlenn, G.C.Thomas, H.L.Schubert, F.G.Whitby, B.R.Howard. Crystal Structures of A Halophilic Archaeal Malate Synthase From Haloferax Volcanii and Comparisons with Isoforms A and G. Bmc Struct.Biol. V. 11 23 2011.
ISSN: ESSN 1472-6807
PubMed: 21569248
DOI: 10.1186/1472-6807-11-23