Potassium in PDB 3n5k: Structure of the (Sr)CA2+-Atpase E2-ALF4- Form

All present enzymatic activity of Structure of the (Sr)CA2+-Atpase E2-ALF4- Form:
3.6.3.8;

The structure of Structure of the (Sr)CA2+-Atpase E2-ALF4- Form, PDB code: 3n5k was solved by M.Bublitz, C.Olesen, H.Poulsen, J.P.Morth, J.V.Moller, P.Nissen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	72.00 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	101.930, 109.420, 276.092, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 21.6

The structure of Structure of the (Sr)CA2+-Atpase E2-ALF4- Form also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Magnesium	(Mg)	2 atoms
Aluminium	(Al)	2 atoms

The binding sites of Potassium atom in the Structure of the (Sr)CA2+-Atpase E2-ALF4- Form (pdb code 3n5k). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Structure of the (Sr)CA2+-Atpase E2-ALF4- Form, PDB code: 3n5k:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Structure of the (Sr)CA2+-Atpase E2-ALF4- Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of the (Sr)CA2+-Atpase E2-ALF4- Form within 5.0Å range: probe atom residue distance (Å) B Occ A:K2003 b:36.3 occ:1.00 O A:ALA714 2.7 32.3 1.0 O A:LEU711 2.8 26.7 1.0 OE1 A:GLU732 2.8 40.9 1.0 O A:LYS712 2.9 28.0 1.0 O A:HOH1107 3.1 36.1 1.0 OE1 A:GLN244 3.1 77.8 1.0 CD A:GLU732 3.4 39.9 1.0 C A:LYS712 3.5 30.7 1.0 OE2 A:GLU732 3.5 43.2 1.0 NE2 A:GLN244 3.5 77.0 1.0 CD A:GLN244 3.7 81.8 1.0 CA A:LYS712 3.7 32.9 1.0 C A:LEU711 3.8 31.3 1.0 C A:ALA714 3.9 37.0 1.0 N A:GLY717 3.9 27.2 1.0 O A:GLU715 4.0 36.4 1.0 O A:HOH1099 4.1 42.3 1.0 N A:ALA714 4.2 31.4 1.0 N A:LYS712 4.2 29.2 1.0 C A:GLU715 4.3 36.8 1.0 N A:LYS713 4.4 27.9 1.0 C A:LYS713 4.5 29.7 1.0 CA A:ALA714 4.5 33.0 1.0 CA A:GLY717 4.6 25.3 1.0 C A:ILE716 4.6 30.6 1.0 O A:ALA730 4.6 36.7 1.0 N A:ILE716 4.6 36.7 1.0 CA A:ILE716 4.8 33.1 1.0 CG A:GLU732 4.8 34.0 1.0 N A:GLU715 4.8 35.7 1.0 CA A:GLU715 4.8 33.9 1.0 O A:LYS713 4.9 31.7 1.0 CB A:ALA714 4.9 31.4 1.0 CA A:LYS713 5.0 27.5 1.0

Potassium binding site 2 out of 2 in the Structure of the (Sr)CA2+-Atpase E2-ALF4- Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of the (Sr)CA2+-Atpase E2-ALF4- Form within 5.0Å range: probe atom residue distance (Å) B Occ B:K2003 b:40.5 occ:1.00 O B:ALA714 2.7 28.4 1.0 O B:LEU711 2.8 28.3 1.0 OE1 B:GLU732 2.9 39.0 1.0 O B:LYS712 3.0 36.5 1.0 O B:HOH1046 3.0 43.3 1.0 O B:HOH1253 3.3 77.2 1.0 OE2 B:GLU732 3.4 40.1 1.0 CD B:GLU732 3.4 36.3 1.0 C B:LYS712 3.5 35.7 1.0 CA B:LYS712 3.8 33.2 1.0 C B:ALA714 3.8 32.6 1.0 N B:GLY717 3.9 33.8 1.0 C B:LEU711 3.9 30.7 1.0 O B:HOH1026 3.9 40.9 1.0 O B:GLU715 4.0 39.4 1.0 C B:GLU715 4.2 37.2 1.0 N B:ALA714 4.2 27.4 1.0 N B:LYS712 4.3 28.4 1.0 N B:LYS713 4.5 36.0 1.0 C B:ILE716 4.5 33.7 1.0 N B:ILE716 4.5 31.5 1.0 O B:ALA730 4.5 37.7 1.0 CA B:ALA714 4.6 28.5 1.0 CA B:GLY717 4.6 33.5 1.0 C B:LYS713 4.6 35.5 1.0 CA B:ILE716 4.6 31.6 1.0 CG B:GLU732 4.7 31.1 1.0 N B:GLU715 4.8 30.8 1.0 CA B:GLU715 4.8 34.1 1.0 CB B:ALA714 5.0 28.2 1.0

M.Bublitz, M.Musgaard, H.Poulsen, L.Thogersen, C.Olesen, B.Schiott, J.P.Morth, J.V.Moller, P.Nissen. Ion Pathways in the Sarcoplasmic Reticulum CA2+-Atpase. J.Biol.Chem. V. 288 10759 2013.
ISSN: ISSN 0021-9258
PubMed: 23400778
DOI: 10.1074/JBC.R112.436550