Potassium in PDB 3ifx: Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1

The structure of Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1, PDB code: 3ifx was solved by J.A.Cieslak, P.J.Focia, A.Gross, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.84 / 3.56
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	130.970, 76.630, 112.970, 90.00, 125.83, 90.00
R / Rfree (%)	27.1 / 30.2

The binding sites of Potassium atom in the Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1 (pdb code 3ifx). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1, PDB code: 3ifx:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K202 b:2.0 occ:1.00 K B:K201 2.4 29.2 1.0 O B:THR75 2.5 59.6 1.0 O D:THR75 2.7 59.6 1.0 O C:THR75 2.8 0.7 1.0 OG1 B:THR75 2.9 58.1 1.0 O A:THR75 3.0 0.6 1.0 OG1 D:THR75 3.1 58.1 1.0 OG1 C:THR75 3.4 98.6 1.0 OG1 A:THR75 3.4 43.8 1.0 C B:THR75 3.5 60.3 1.0 CB B:THR75 3.6 57.5 1.0 CG2 A:THR75 3.7 61.2 1.0 C D:THR75 3.8 60.2 1.0 CB D:THR75 3.8 57.6 1.0 C41 B:TBA203 3.9 2.0 0.5 C C:THR75 4.0 55.8 1.0 C A:THR75 4.1 2.0 1.0 CG2 C:THR75 4.1 85.3 1.0 CB A:THR75 4.1 2.0 1.0 CA B:THR75 4.2 58.0 1.0 CB C:THR75 4.3 5.2 1.0 CA D:THR75 4.5 58.0 1.0 N B:VAL76 4.5 63.4 1.0 C21 B:TBA203 4.5 0.8 0.5 O C:THR74 4.6 4.9 1.0 CA B:VAL76 4.7 66.4 1.0 N D:VAL76 4.8 63.4 1.0 O D:THR74 4.8 53.4 1.0 CA A:THR75 4.8 2.0 1.0 O B:VAL76 4.8 67.2 1.0 CA C:THR75 4.8 2.0 1.0 N1 B:TBA203 4.9 9.5 0.5 O A:VAL76 4.9 50.6 1.0 O A:THR74 4.9 6.3 1.0 C42 B:TBA203 4.9 13.1 0.5 N C:VAL76 4.9 65.6 1.0 CA D:VAL76 4.9 66.3 1.0 O C:VAL76 5.0 67.0 1.0 CG2 B:THR75 5.0 57.1 1.0 CA C:VAL76 5.0 0.8 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the Spin-Labeled Kcsa Mutant V48R1 within 5.0Å range: probe atom residue distance (Å) B Occ B:K201 b:29.2 occ:1.00 O B:THR75 2.2 59.6 1.0 K A:K202 2.4 2.0 1.0 O D:THR75 2.4 59.6 1.0 O B:VAL76 2.7 67.2 1.0 O A:THR75 2.7 0.6 1.0 O C:THR75 2.8 0.7 1.0 O A:VAL76 2.9 50.6 1.0 O C:VAL76 2.9 67.0 1.0 O D:VAL76 3.1 67.2 1.0 C B:THR75 3.3 60.3 1.0 C B:VAL76 3.4 67.5 1.0 CA B:VAL76 3.5 66.4 1.0 C D:THR75 3.5 60.2 1.0 C C:VAL76 3.7 83.7 1.0 C D:VAL76 3.8 67.5 1.0 CA D:VAL76 3.8 66.3 1.0 C A:VAL76 3.8 71.9 1.0 C C:THR75 3.8 55.8 1.0 N B:VAL76 3.9 63.4 1.0 C A:THR75 3.9 2.0 1.0 CA C:VAL76 3.9 0.8 1.0 OG1 C:THR75 4.0 98.6 1.0 CA A:VAL76 4.0 97.6 1.0 N D:VAL76 4.1 63.4 1.0 O D:HOH204 4.1 8.0 1.0 OG1 A:THR75 4.3 43.8 1.0 N C:VAL76 4.3 65.6 1.0 OG1 B:THR75 4.4 58.1 1.0 N A:VAL76 4.4 19.8 1.0 OG1 D:THR75 4.6 58.1 1.0 CA B:THR75 4.6 58.0 1.0 N B:GLY77 4.7 70.0 1.0 N C:GLY77 4.7 0.7 1.0 CA D:THR75 4.8 58.0 1.0 CB B:THR75 4.8 57.5 1.0 CB D:THR75 4.8 57.6 1.0 CB B:VAL76 4.9 66.8 1.0 N D:GLY77 5.0 70.0 1.0 N A:GLY77 5.0 73.4 1.0

J.A.Cieslak, P.J.Focia, A.Gross. Electron Spin-Echo Envelope Modulation (Eseem) Reveals Water and Phosphate Interactions with the Kcsa Potassium Channel Biochemistry V. 49 1486 2010.
ISSN: ISSN 0006-2960
PubMed: 20092291
DOI: 10.1021/BI9016523