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Potassium in PDB 3h8g: Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida

Enzymatic activity of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida

All present enzymatic activity of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida:
3.4.11.1;

Protein crystallography data

The structure of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida, PDB code: 3h8g was solved by A.Kale, B.W.Dijkstra, T.Sonke, A.M.W.H.Thunnissen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 38.63 / 1.50
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 95.976, 95.989, 95.998, 100.82, 107.78, 93.23
R / Rfree (%) 14.9 / 17.3

Other elements in 3h8g:

The structure of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida also contains other interesting chemical elements:

Manganese (Mn) 6 atoms
Zinc (Zn) 6 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida (pdb code 3h8g). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 6 binding sites of Potassium where determined in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida, PDB code: 3h8g:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6;

Potassium binding site 1 out of 6 in 3h8g

Go back to Potassium Binding Sites List in 3h8g
Potassium binding site 1 out of 6 in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K503

b:6.1
occ:1.00
O A:LEU192 2.7 5.6 1.0
O A:HOH514 2.7 8.2 1.0
O A:LYS288 2.8 6.9 1.0
O A:LEU189 2.8 7.5 1.0
O A:GLY190 2.8 6.8 1.0
SD A:MET291 3.3 7.2 1.0
C A:GLY190 3.4 6.2 1.0
CA A:GLY190 3.8 6.1 1.0
C A:LEU192 3.8 5.5 1.0
C A:LYS288 3.8 6.4 1.0
C A:LEU189 3.9 7.2 1.0
SG A:CYS197 4.0 10.9 1.0
CB A:CYS197 4.0 6.8 1.0
CB A:LYS288 4.0 7.2 1.0
CG A:MET291 4.1 5.5 1.0
N A:LEU192 4.1 5.7 1.0
CE A:MET291 4.2 6.2 1.0
CG A:LYS288 4.3 7.7 1.0
CA A:LEU192 4.3 5.2 1.0
N A:GLY190 4.4 6.1 1.0
CA A:LYS288 4.4 6.6 1.0
N A:ASN191 4.4 6.1 1.0
CB A:LEU192 4.5 6.6 1.0
C A:ASN191 4.5 6.0 1.0
CD A:LYS288 4.6 7.3 1.0
N A:TYR289 4.8 5.9 1.0
O A:THR270 4.9 5.5 1.0
N A:PRO193 4.9 5.0 1.0
CA A:ASN191 5.0 6.2 1.0
C A:PRO193 5.0 5.2 1.0

Potassium binding site 2 out of 6 in 3h8g

Go back to Potassium Binding Sites List in 3h8g
Potassium binding site 2 out of 6 in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K503

b:6.7
occ:1.00
O B:LEU192 2.7 7.2 1.0
O B:HOH524 2.7 8.1 1.0
O B:GLY190 2.7 7.1 1.0
O B:LYS288 2.8 7.5 1.0
O B:LEU189 2.9 7.4 1.0
SD B:MET291 3.3 7.5 1.0
C B:GLY190 3.4 7.2 1.0
CA B:GLY190 3.8 6.8 1.0
C B:LEU192 3.8 6.3 1.0
C B:LYS288 3.8 6.8 1.0
SG B:CYS197 3.9 10.5 1.0
C B:LEU189 4.0 7.7 1.0
CB B:CYS197 4.0 7.0 1.0
CB B:LYS288 4.0 6.5 1.0
CG B:MET291 4.1 7.0 1.0
N B:LEU192 4.1 6.7 1.0
CG B:LYS288 4.3 7.3 1.0
CE B:MET291 4.3 6.3 1.0
CA B:LYS288 4.3 7.2 1.0
N B:GLY190 4.4 5.7 1.0
CA B:LEU192 4.4 7.3 1.0
N B:ASN191 4.4 6.8 1.0
C B:ASN191 4.5 6.8 1.0
CB B:LEU192 4.6 7.8 1.0
CD B:LYS288 4.6 7.7 1.0
N B:TYR289 4.8 5.7 1.0
N B:PRO193 4.9 6.4 1.0
O B:THR270 4.9 5.6 1.0
CA B:ASN191 4.9 6.7 1.0

Potassium binding site 3 out of 6 in 3h8g

Go back to Potassium Binding Sites List in 3h8g
Potassium binding site 3 out of 6 in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K503

b:6.3
occ:1.00
O C:LEU192 2.7 6.0 1.0
O C:HOH518 2.7 7.0 1.0
O C:GLY190 2.8 5.6 1.0
O C:LYS288 2.8 6.6 1.0
O C:LEU189 2.9 7.1 1.0
SD C:MET291 3.3 7.0 1.0
C C:GLY190 3.4 6.1 1.0
C C:LEU192 3.8 5.3 1.0
CA C:GLY190 3.8 6.2 1.0
C C:LYS288 3.8 6.9 1.0
C C:LEU189 3.9 7.3 1.0
SG C:CYS197 4.0 10.5 1.0
CB C:LYS288 4.0 7.8 1.0
CG C:MET291 4.0 6.5 1.0
N C:LEU192 4.1 5.6 1.0
CB C:CYS197 4.1 7.9 1.0
CE C:MET291 4.2 6.9 1.0
CG C:LYS288 4.3 6.7 1.0
CA C:LYS288 4.4 7.3 1.0
N C:GLY190 4.4 6.5 1.0
CA C:LEU192 4.4 5.5 1.0
N C:ASN191 4.4 5.2 1.0
C C:ASN191 4.6 5.5 1.0
CB C:LEU192 4.6 7.2 1.0
CD C:LYS288 4.6 7.4 1.0
N C:TYR289 4.8 4.9 1.0
N C:PRO193 4.9 4.7 1.0
O C:THR270 4.9 6.3 1.0
CA C:ASN191 5.0 4.5 1.0
C C:PRO193 5.0 5.3 1.0

Potassium binding site 4 out of 6 in 3h8g

Go back to Potassium Binding Sites List in 3h8g
Potassium binding site 4 out of 6 in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida within 5.0Å range:
probe atom residue distance (Å) B Occ
D:K503

b:6.9
occ:1.00
O D:LEU192 2.7 6.9 1.0
O D:HOH526 2.7 8.2 1.0
O D:GLY190 2.8 6.6 1.0
O D:LEU189 2.8 8.0 1.0
O D:LYS288 2.9 7.4 1.0
SD D:MET291 3.3 7.8 1.0
C D:GLY190 3.4 6.1 1.0
C D:LEU192 3.8 6.5 1.0
CA D:GLY190 3.8 6.7 1.0
C D:LYS288 3.8 7.2 1.0
SG D:CYS197 3.9 11.2 1.0
C D:LEU189 3.9 7.3 1.0
CG D:MET291 4.0 6.2 1.0
CB D:LYS288 4.0 7.6 1.0
N D:LEU192 4.1 7.2 1.0
CB D:CYS197 4.1 8.4 1.0
CE D:MET291 4.3 5.8 1.0
CA D:LEU192 4.3 6.9 1.0
N D:GLY190 4.3 6.1 1.0
CG D:LYS288 4.3 6.2 1.0
CA D:LYS288 4.4 7.6 1.0
N D:ASN191 4.4 6.5 1.0
CB D:LEU192 4.5 6.8 1.0
C D:ASN191 4.5 6.6 1.0
CD D:LYS288 4.6 8.3 1.0
N D:TYR289 4.8 6.2 1.0
N D:PRO193 4.9 6.5 1.0
O D:THR270 4.9 6.0 1.0
CA D:ASN191 5.0 6.3 1.0

Potassium binding site 5 out of 6 in 3h8g

Go back to Potassium Binding Sites List in 3h8g
Potassium binding site 5 out of 6 in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K503

b:6.4
occ:1.00
O E:LEU192 2.6 6.4 1.0
O E:HOH510 2.7 7.8 1.0
O E:GLY190 2.8 7.1 1.0
O E:LYS288 2.8 6.9 1.0
O E:LEU189 2.9 6.4 1.0
SD E:MET291 3.3 7.1 1.0
C E:GLY190 3.5 6.7 1.0
C E:LEU192 3.7 6.3 1.0
CA E:GLY190 3.8 6.2 1.0
C E:LYS288 3.8 6.8 1.0
SG E:CYS197 3.8 10.3 1.0
C E:LEU189 4.0 6.5 1.0
CB E:LYS288 4.0 6.2 1.0
CG E:MET291 4.0 5.5 1.0
CB E:CYS197 4.1 6.8 1.0
N E:LEU192 4.1 5.8 1.0
CE E:MET291 4.3 5.5 1.0
CA E:LEU192 4.3 6.2 1.0
CG E:LYS288 4.3 6.8 1.0
N E:GLY190 4.4 5.6 1.0
CA E:LYS288 4.4 6.6 1.0
N E:ASN191 4.5 6.9 1.0
CB E:LEU192 4.5 7.8 1.0
C E:ASN191 4.6 6.2 1.0
CD E:LYS288 4.6 7.2 1.0
N E:TYR289 4.8 5.0 1.0
O E:THR270 4.9 5.8 1.0
N E:PRO193 4.9 5.6 1.0
CA E:ASN191 5.0 6.4 1.0
C E:PRO193 5.0 6.1 1.0

Potassium binding site 6 out of 6 in 3h8g

Go back to Potassium Binding Sites List in 3h8g
Potassium binding site 6 out of 6 in the Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Bestatin Complex Structure of Leucine Aminopeptidase From Pseudomonas Putida within 5.0Å range:
probe atom residue distance (Å) B Occ
F:K503

b:6.2
occ:1.00
O F:LEU192 2.5 6.0 1.0
O F:GLY190 2.7 5.9 1.0
O F:HOH518 2.7 6.9 1.0
O F:LYS288 2.8 6.5 1.0
O F:LEU189 2.9 6.5 1.0
SD F:MET291 3.3 7.5 1.0
C F:GLY190 3.4 5.3 1.0
C F:LEU192 3.7 5.4 1.0
CA F:GLY190 3.7 4.9 1.0
C F:LYS288 3.8 6.1 1.0
SG F:CYS197 3.9 10.4 1.0
C F:LEU189 4.0 6.0 1.0
CG F:MET291 4.0 5.8 1.0
CB F:LYS288 4.1 6.7 1.0
CB F:CYS197 4.1 6.3 1.0
N F:LEU192 4.2 5.2 1.0
CE F:MET291 4.2 6.4 1.0
CG F:LYS288 4.3 7.2 1.0
CA F:LEU192 4.3 6.0 1.0
CA F:LYS288 4.4 6.4 1.0
N F:GLY190 4.4 5.8 1.0
N F:ASN191 4.4 6.2 1.0
CB F:LEU192 4.5 7.3 1.0
C F:ASN191 4.5 5.9 1.0
CD F:LYS288 4.6 8.4 1.0
N F:TYR289 4.8 5.2 1.0
N F:PRO193 4.9 5.1 1.0
O F:THR270 4.9 5.2 1.0
CA F:ASN191 5.0 4.9 1.0

Reference:

A.Kale, T.Pijning, T.Sonke, B.W.Dijkstra, A.M.Thunnissen. Crystal Structure of the Leucine Aminopeptidase From Pseudomonas Putida Reveals the Molecular Basis For Its Enantioselectivity and Broad Substrate Specificity. J.Mol.Biol. V. 398 703 2010.
ISSN: ISSN 0022-2836
PubMed: 20359484
DOI: 10.1016/J.JMB.2010.03.042
Page generated: Sun Dec 13 23:19:21 2020

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