Potassium in PDB 3gko: Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent

All present enzymatic activity of Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent:
1.7.3.3;

The structure of Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent, PDB code: 3gko was solved by V.Delfosse, M.Giffard, G.Sciara, F.Bonnete, C.Mayer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.52 / 1.60
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	80.063, 95.284, 104.534, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / 19.9

The binding sites of Potassium atom in the Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent (pdb code 3gko). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent, PDB code: 3gko:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent within 5.0Å range: probe atom residue distance (Å) B Occ A:K303 b:20.8 occ:1.00 O A:ILE88 2.8 15.3 1.0 O A:ILE94 2.9 11.9 1.0 O A:TYR91 3.0 12.7 1.0 OE1 A:GLU136 3.0 22.4 1.0 O A:ASN92 3.2 16.4 1.0 O A:HOH612 3.5 29.3 1.0 C A:ASN92 3.5 14.0 1.0 CD A:GLU136 3.6 22.3 1.0 CA A:ASN92 3.6 15.6 1.0 OE2 A:GLU136 3.8 23.0 1.0 C A:ILE88 3.8 13.7 1.0 O A:HOH524 3.9 39.6 1.0 C A:TYR91 3.9 12.8 1.0 C A:ILE94 3.9 11.8 1.0 ND1 A:HIS95 4.0 15.5 1.0 CA A:ILE88 4.2 13.6 1.0 N A:ASN92 4.2 13.8 1.0 N A:ILE94 4.3 11.8 1.0 CE1 A:HIS95 4.3 15.0 1.0 N A:HIS93 4.5 13.5 1.0 CB A:ILE88 4.5 14.4 1.0 CA A:ILE94 4.7 11.4 1.0 CG A:GLU136 4.8 19.8 1.0 N A:HIS95 4.8 10.8 1.0 CA A:HIS95 4.8 11.2 1.0 CB A:ASN92 4.9 17.7 1.0 N A:GLU89 4.9 13.7 1.0 C A:HIS93 4.9 12.4 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Urate Oxydase Using Surfactant Poloxamer 188 As A New Crystallizing Agent within 5.0Å range: probe atom residue distance (Å) B Occ A:K304 b:14.4 occ:1.00 ND2 A:ASN254 3.2 8.8 1.0 CA A:GLY286 3.4 8.1 1.0 CB A:ASN254 3.4 7.2 1.0 C4 A:AZA302 3.5 10.4 1.0 C5 A:AZA302 3.6 10.1 1.0 CE1 A:HIS256 3.7 7.2 1.0 CG A:ASN254 3.8 9.1 1.0 N3 A:AZA302 3.8 8.9 1.0 N9 A:AZA302 3.9 10.7 1.0 C6 A:AZA302 3.9 9.5 1.0 N7 A:AZA302 3.9 10.6 1.0 N A:GLY286 4.1 7.3 1.0 N8 A:AZA302 4.1 10.0 1.0 C2 A:AZA302 4.2 8.6 1.0 CD1 A:ILE288 4.2 10.9 1.0 N1 A:AZA302 4.2 9.0 1.0 NE2 A:HIS256 4.2 10.0 1.0 C A:GLY286 4.3 8.4 1.0 O6 A:AZA302 4.5 10.4 1.0 CG1 A:ILE288 4.8 10.2 1.0 CA A:ASN254 4.8 6.8 1.0 ND1 A:HIS256 4.8 8.9 1.0 N A:LEU287 4.8 7.1 1.0 O2 A:AZA302 4.9 9.3 1.0 O A:GLY286 4.9 9.0 1.0

M.Giffard, V.Delfosse, G.Sciara, C.Mayer, C.Cambillau, M.El Hajji, B.Castro, F.Bonnete. Surfactant Poloxamer 188 As A New Crystallizing Agent For Urate Oxidase Cryst.Growth Des. V. 9 4199 2009.
ISSN: ISSN 1528-7483
DOI: 10.1021/CG900473N