Potassium in PDB 3den: Structure of E. Coli Dhdps Mutant Y107W

All present enzymatic activity of Structure of E. Coli Dhdps Mutant Y107W:
4.2.1.52;

The structure of Structure of E. Coli Dhdps Mutant Y107W, PDB code: 3den was solved by F.G.Pearce, J.A.Gerrard, M.A.Perugini, G.B.Jameson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.22 / 2.20
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	121.196, 121.196, 110.565, 90.00, 90.00, 120.00
R / Rfree (%)	18.3 / 24.1

The binding sites of Potassium atom in the Structure of E. Coli Dhdps Mutant Y107W (pdb code 3den). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Structure of E. Coli Dhdps Mutant Y107W, PDB code: 3den:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Structure of E. Coli Dhdps Mutant Y107W


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of E. Coli Dhdps Mutant Y107W within 5.0Å range: probe atom residue distance (Å) B Occ A:K293 b:30.9 occ:1.00 O A:VAL154 2.6 32.3 1.0 O A:ALA152 2.6 27.8 1.0 O A:ILE157 2.7 24.9 1.0 O A:HOH501 3.0 34.6 1.0 O A:HOH434 3.1 49.5 1.0 O A:LYS155 3.3 31.7 1.0 C A:ALA152 3.7 27.4 1.0 C A:ILE157 3.7 24.8 1.0 C A:VAL154 3.7 33.3 1.0 C A:LYS155 3.8 32.4 1.0 N A:ILE157 4.1 25.2 1.0 CA A:LYS155 4.1 33.1 1.0 O A:HOH341 4.3 44.3 1.0 CA A:ALA152 4.3 26.7 1.0 N A:LYS155 4.3 33.4 1.0 CA A:ILE157 4.4 25.0 1.0 N A:ASN156 4.6 30.3 1.0 N A:ILE158 4.6 25.4 1.0 CA A:ILE158 4.6 25.4 1.0 N A:VAL154 4.6 33.2 1.0 C A:LYS153 4.6 31.6 1.0 N A:LYS153 4.7 28.8 1.0 CD1 A:PHE181 4.8 19.5 1.0 CG2 A:ILE158 4.8 26.2 1.0 CA A:VAL154 4.8 32.2 1.0 O A:LYS153 4.9 32.0 1.0 CB A:ILE157 4.9 24.1 1.0 O A:HOH406 4.9 43.1 1.0 CA A:LYS153 4.9 30.2 1.0 C A:ASN156 5.0 27.4 1.0 CB A:ALA152 5.0 26.0 1.0

Potassium binding site 2 out of 2 in the Structure of E. Coli Dhdps Mutant Y107W


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of E. Coli Dhdps Mutant Y107W within 5.0Å range: probe atom residue distance (Å) B Occ B:K293 b:37.0 occ:1.00 O B:VAL154 2.6 33.8 1.0 O B:ILE157 2.7 30.7 1.0 O B:ALA152 2.8 30.2 1.0 O B:HOH347 3.2 40.0 1.0 O B:LYS155 3.2 34.6 1.0 O B:HOH450 3.5 55.4 1.0 C B:VAL154 3.7 34.4 1.0 C B:ILE157 3.7 29.6 1.0 C B:ALA152 3.8 29.8 1.0 C B:LYS155 3.8 35.0 1.0 CA B:LYS155 4.1 35.5 1.0 N B:ILE157 4.1 31.9 1.0 CA B:ALA152 4.3 29.5 1.0 N B:LYS155 4.3 35.3 1.0 CA B:ILE157 4.3 30.7 1.0 CG2 B:ILE158 4.5 27.9 1.0 N B:ILE158 4.6 29.4 1.0 N B:VAL154 4.6 34.2 1.0 CA B:ILE158 4.6 28.6 1.0 C B:LYS153 4.7 33.7 1.0 N B:ASN156 4.7 34.4 1.0 CA B:VAL154 4.7 34.0 1.0 N B:LYS153 4.8 30.5 1.0 CB B:ILE157 4.8 30.6 1.0 CD1 B:PHE181 4.8 28.6 1.0 O B:LYS153 4.9 35.0 1.0 CB B:ALA152 4.9 28.5 1.0 C B:ASN156 5.0 33.0 1.0

F.G.Pearce, R.C.J.Dobson, A.Weber, L.A.Lane, M.G.Mccammon, M.A.Squire, M.A.Perugini, G.B.Jameson, C.V.Robinson, J.A.Gerrard. Mutating the Tight-Dimer Interface of Dihydrodipicolinate Synthase Disrupts the Enzyme Quaternary Structure: Toward A Monomeric Enzyme Biochemistry V. 47 12108 2008.
ISSN: ISSN 0006-2960
PubMed: 18937497
DOI: 10.1021/BI801094T