Potassium in PDB 3bjf: Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein

All present enzymatic activity of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein:
2.7.1.40;

The structure of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein, PDB code: 3bjf was solved by N.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.29 / 2.03
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	80.756, 81.458, 106.885, 74.63, 69.91, 66.10
R / Rfree (%)	20.8 / 24.6

The structure of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Potassium atom in the Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein (pdb code 3bjf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein, PDB code: 3bjf:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:K601 b:75.8 occ:1.00 O A:HOH947 3.0 27.4 1.0 O1 A:OXL901 3.2 31.6 1.0 OD2 A:ASP296 3.2 23.1 1.0 O A:HOH964 3.5 28.3 1.0 O4 A:OXL901 3.5 26.6 1.0 C1 A:OXL901 3.8 30.5 1.0 C2 A:OXL901 4.0 30.0 1.0 O A:HOH902 4.2 21.8 1.0 CG A:ASP296 4.4 25.2 1.0 OE2 A:GLU272 4.6 22.6 1.0 O3 A:OXL901 4.7 33.7 1.0 O A:HOH1020 4.9 27.8 1.0 O2 A:OXL901 5.0 28.7 1.0

Potassium binding site 2 out of 4 in the Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein within 5.0Å range: probe atom residue distance (Å) B Occ B:K602 b:45.4 occ:1.00 OE1 B:GLU272 2.9 16.7 1.0 O B:HOH904 2.9 13.9 1.0 OE2 B:GLU272 3.0 14.8 1.0 O B:HOH903 3.0 16.5 1.0 O1 B:OXL902 3.1 24.5 1.0 CD B:GLU272 3.3 17.6 1.0 OD2 B:ASP296 3.4 22.7 1.0 NZ B:LYS270 3.4 13.0 1.0 OG B:SER243 3.5 14.2 1.0 CE1 B:PHE244 3.5 23.9 1.0 CZ B:PHE244 3.6 24.6 1.0 CB B:SER243 3.7 15.1 1.0 O B:HOH1009 3.8 27.9 1.0 O4 B:OXL902 4.1 25.2 1.0 CD1 B:PHE244 4.2 21.8 1.0 C1 B:OXL902 4.2 26.1 1.0 O B:HOH1027 4.2 26.1 1.0 CE B:LYS270 4.2 14.9 1.0 CE2 B:PHE244 4.4 22.9 1.0 CG B:ASP296 4.4 16.2 1.0 MG B:MG702 4.5 8.6 1.0 CA B:SER243 4.7 16.6 1.0 C2 B:OXL902 4.7 23.5 1.0 OE1 B:GLU118 4.7 26.0 1.0 CG B:GLU272 4.8 17.3 1.0 CG B:PHE244 4.8 19.6 1.0 O B:LYS115 4.9 17.4 1.0 CD2 B:PHE244 4.9 19.5 1.0 N B:PHE244 5.0 17.0 1.0

Potassium binding site 3 out of 4 in the Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein within 5.0Å range: probe atom residue distance (Å) B Occ C:K603 b:61.4 occ:1.00 O3 C:OXL903 2.9 23.1 1.0 OD2 C:ASP296 3.0 21.8 1.0 O C:HOH904 3.1 19.6 1.0 OE2 C:GLU272 3.1 20.6 1.0 OE1 C:GLU272 3.1 18.3 1.0 CD C:GLU272 3.4 20.6 1.0 NZ C:LYS270 3.5 22.1 1.0 CZ C:PHE244 3.6 26.8 1.0 O C:HOH967 3.7 19.5 1.0 CE1 C:PHE244 3.7 26.3 1.0 OG C:SER243 3.8 20.0 1.0 O2 C:OXL903 3.9 23.1 1.0 C1 C:OXL903 4.0 26.1 1.0 CB C:SER243 4.1 17.1 1.0 CG C:ASP296 4.2 19.0 1.0 CE C:LYS270 4.3 19.9 1.0 CE2 C:PHE244 4.3 26.2 1.0 C2 C:OXL903 4.5 22.5 1.0 CD1 C:PHE244 4.5 21.2 1.0 MG C:MG703 4.5 12.7 1.0 OE1 C:GLU118 4.6 27.3 1.0 CG C:GLU272 4.9 20.4 1.0 CB C:ASP296 4.9 19.8 1.0

Potassium binding site 4 out of 4 in the Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Pyruvate Kinase M2 Is A Phosphotyrosine Binding Protein within 5.0Å range: probe atom residue distance (Å) B Occ D:K604 b:49.6 occ:1.00 O D:HOH905 2.8 17.6 1.0 OE1 D:GLU272 2.9 19.0 1.0 O D:HOH992 3.0 25.8 1.0 OE2 D:GLU272 3.1 21.7 1.0 O4 D:OXL904 3.1 32.0 1.0 OD2 D:ASP296 3.3 22.8 1.0 NZ D:LYS270 3.3 18.5 1.0 CD D:GLU272 3.4 20.8 1.0 OG D:SER243 3.5 20.3 1.0 CE1 D:PHE244 3.6 33.4 1.0 CZ D:PHE244 3.6 33.6 1.0 O D:HOH997 3.7 20.3 1.0 CB D:SER243 3.7 19.6 1.0 CD1 D:PHE244 4.2 32.5 1.0 CE D:LYS270 4.2 20.4 1.0 MG D:MG704 4.2 15.4 1.0 C2 D:OXL904 4.3 33.3 1.0 O1 D:OXL904 4.3 31.4 1.0 CE2 D:PHE244 4.3 32.7 1.0 CG D:ASP296 4.4 22.9 1.0 OE1 D:GLU118 4.6 32.5 1.0 CA D:SER243 4.7 21.0 1.0 O D:LYS115 4.8 21.6 1.0 CG D:GLU272 4.8 22.4 1.0 CG D:PHE244 4.8 30.8 1.0 C1 D:OXL904 4.9 32.0 1.0 CD2 D:PHE244 4.9 32.3 1.0

H.R.Christofk, M.G.Vander Heiden, N.Wu, J.M.Asara, L.C.Cantley. Pyruvate Kinase M2 Is A Phosphotyrosine-Binding Protein. Nature V. 452 181 2008.
ISSN: ISSN 0028-0836
PubMed: 18337815
DOI: 10.1038/NATURE06667