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Potassium in PDB 2ycn: Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine

Enzymatic activity of Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine

All present enzymatic activity of Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine:
4.1.99.2;

Protein crystallography data

The structure of Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine, PDB code: 2ycn was solved by D.Milic, T.V.Demidkina, N.G.Faleev, R.S.Phillips, D.Matkovic-Calogovic, A.A.Antson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 98.06 / 2.04
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 133.635, 144.397, 59.660, 90.00, 90.00, 90.00
R / Rfree (%) 16.3 / 20.9

Other elements in 2ycn:

The structure of Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine also contains other interesting chemical elements:

Fluorine (F) 3 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine (pdb code 2ycn). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine, PDB code: 2ycn:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 2ycn

Go back to Potassium Binding Sites List in 2ycn
Potassium binding site 1 out of 2 in the Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K500

b:22.9
occ:1.00
 O B:HOH2102 2.7 22.5 1.0
O B:HOH2285 2.7 14.6 1.0
OE1 B:GLU69 2.8 20.5 1.0
O A:GLY52 2.8 19.3 1.0
O B:GLU69 3.1 29.1 1.0
O A:ASN262 3.1 25.1 1.0
O A:HOH2210 3.2 27.6 1.0
C A:GLY52 3.6 20.0 1.0
CB B:GLU69 3.7 16.6 1.0
C B:GLU69 3.8 24.7 1.0
CD B:GLU69 3.9 20.1 1.0
CA B:GLU69 3.9 20.3 1.0
CA A:GLY52 4.0 20.3 1.0
C A:ASN262 4.0 20.6 1.0
CB A:ASN262 4.1 17.2 1.0
O B:HOH2104 4.1 14.8 1.0
CA A:ASN262 4.3 19.7 1.0
CG B:GLU69 4.3 15.2 1.0
CA B:ALA295 4.4 21.9 1.0
N B:GLY296 4.5 19.8 1.0
N A:THR53 4.8 18.1 1.0
O B:ALA70 4.8 26.5 1.0
CE A:LYS256 4.8 23.5 1.0
ND2 A:ASN262 4.9 18.9 1.0
O B:LEU294 4.9 26.2 1.0
CB B:ALA295 4.9 20.3 1.0
O A:SER51 4.9 20.6 1.0
OE2 B:GLU69 5.0 18.6 1.0
CG A:ASN262 5.0 21.8 1.0
C B:ALA295 5.0 21.1 1.0

Potassium binding site 2 out of 2 in 2ycn

Go back to Potassium Binding Sites List in 2ycn
Potassium binding site 2 out of 2 in the Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Y71F Mutant of Tyrosine Phenol-Lyase From Citrobacter Freundii in Complex with Quinonoid Intermediate Formed with 3-Fluoro-L-Tyrosine within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K500

b:21.4
occ:1.00
 O A:HOH2223 2.6 9.8 1.0
OE1 A:GLU69 2.8 20.4 1.0
O B:GLY52 2.9 19.1 1.0
O B:ASN262 3.0 18.9 1.0
O B:HOH2261 3.1 19.9 1.0
O B:HOH2084 3.2 17.1 1.0
O A:GLU69 3.2 24.9 1.0
C B:GLY52 3.7 19.6 1.0
CB A:GLU69 3.8 16.2 1.0
CD A:GLU69 3.9 19.2 1.0
C A:GLU69 4.0 20.8 1.0
C B:ASN262 4.0 18.5 1.0
CB B:ASN262 4.0 17.1 1.0
CA A:GLU69 4.0 18.7 1.0
CA B:GLY52 4.1 18.5 1.0
O A:HOH2085 4.2 7.5 1.0
CA A:ALA295 4.3 20.3 1.0
CA B:ASN262 4.3 17.8 1.0
CG A:GLU69 4.4 17.9 1.0
N A:GLY296 4.5 18.6 1.0
CB A:ALA295 4.8 18.2 1.0
O A:LEU294 4.8 23.6 1.0
N B:THR53 4.8 18.8 1.0
CE B:LYS256 4.8 20.9 1.0
ND2 B:ASN262 4.9 19.4 1.0
C A:ALA295 4.9 19.8 1.0
CG B:ASN262 4.9 22.7 1.0

Reference:

D.Milic, T.V.Demidkina, N.G.Faleev, R.S.Phillips, D.Matkovic-Calogovic, A.A.Antson. Crystallographic Snapshots of Tyrosine Phenol-Lyase Show That Substrate Strain Plays A Role in C-C Bond Cleavage J.Am.Chem.Soc. V. 133 16468 2011.
ISSN: ISSN 0002-7863
PubMed: 21899319
DOI: 10.1021/JA203361G
Page generated: Mon Aug 12 07:42:13 2024

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