Potassium in PDB 2w0f: Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium

The structure of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium, PDB code: 2w0f was solved by M.J.Lenaeus, P.J.Focia, T.Wagner, A.Gross, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.4
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	155.760, 155.760, 75.889, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 24.1

The structure of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium also contains other interesting chemical elements:

Cobalt

(Co)

1 atom

The binding sites of Potassium atom in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium (pdb code 2w0f). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 7 binding sites of Potassium where determined in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium, PDB code: 2w0f:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7;

Potassium binding site 1 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1129 b:40.6 occ:0.20 K C:K1133 0.3 17.0 0.1 O C:HOH2019 2.2 45.8 0.2 K C:K1132 2.2 28.5 0.1 O C:THR75 2.3 36.9 0.2 O C:THR75 2.7 40.9 0.8 K C:K1131 2.7 69.0 0.2 OG1 C:THR75 2.7 39.3 0.8 CB C:THR75 3.4 39.5 0.8 C C:THR75 3.5 36.9 0.2 OG1 C:THR75 3.6 36.8 0.2 C C:THR75 3.7 40.5 0.8 CB C:THR75 3.9 37.1 0.2 CA C:THR75 4.2 39.8 0.8 CA C:THR75 4.3 37.2 0.2 O C:HOH2018 4.4 64.7 0.8 N C:VAL76 4.4 36.4 0.2 CG2 C:THR75 4.6 39.2 0.8 CAA C:HX01128 4.6 45.1 0.1 CAZ C:HX01128 4.6 45.1 0.1 CA C:VAL76 4.6 35.9 0.2 CAA C:HX01128 4.6 44.1 0.1 CAZ C:HX01128 4.6 44.1 0.1 N C:VAL76 4.8 40.6 0.8

Potassium binding site 2 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1130 b:60.1 occ:0.20 K C:K1134 0.9 23.7 0.1 O C:TYR78 2.2 37.8 0.2 O C:GLY77 3.0 42.8 0.8 K C:K1135 3.0 38.1 0.1 C C:TYR78 3.2 37.7 0.2 O C:TYR78 3.5 39.7 0.8 O C:GLY77 3.6 36.5 0.2 O C:HOH2004 3.7 49.2 0.2 C C:TYR78 3.7 39.8 0.8 C C:GLY77 3.8 42.3 0.8 O C:HOH2020 4.1 23.6 0.1 CA C:TYR78 4.1 37.4 0.2 N C:GLY79 4.2 38.8 1.0 CA C:GLY79 4.4 37.9 1.0 CA C:TYR78 4.4 39.8 0.8 N C:TYR78 4.5 41.0 0.8 C C:GLY77 4.6 36.7 0.2 CA C:GLY77 4.7 41.9 0.8 N C:TYR78 4.9 37.1 0.2

Potassium binding site 3 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1131 b:69.0 occ:0.20 K C:K1132 0.5 28.5 0.1 O C:HOH2020 2.5 23.6 0.1 O C:THR75 2.7 40.9 0.8 K C:K1129 2.7 40.6 0.2 O C:THR75 2.9 36.9 0.2 O C:VAL76 2.9 35.6 0.2 K C:K1133 3.0 17.0 0.1 K C:K1135 3.6 38.1 0.1 C C:VAL76 3.6 36.1 0.2 N C:GLY77 3.7 42.4 0.8 C C:THR75 3.8 40.5 0.8 CA C:GLY77 3.8 41.9 0.8 C C:THR75 3.9 36.9 0.2 C C:VAL76 3.9 42.2 0.8 CA C:VAL76 3.9 35.9 0.2 O C:VAL76 4.2 42.4 0.8 N C:VAL76 4.3 36.4 0.2 CA C:VAL76 4.5 41.2 0.8 N C:VAL76 4.6 40.6 0.8 OG1 C:THR75 4.7 39.3 0.8 N C:GLY77 4.7 36.2 0.2 CB C:THR75 4.7 39.5 0.8 CA C:THR75 4.8 39.8 0.8 C C:GLY77 4.9 42.3 0.8 O C:HOH2019 4.9 45.8 0.2 O C:GLY77 4.9 42.8 0.8

Potassium binding site 4 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1132 b:28.5 occ:0.05 K C:K1131 0.5 69.0 0.2 K C:K1129 2.2 40.6 0.2 O C:THR75 2.5 40.9 0.8 K C:K1133 2.5 17.0 0.1 O C:THR75 2.5 36.9 0.2 O C:HOH2020 3.0 23.6 0.1 O C:VAL76 3.3 35.6 0.2 C C:THR75 3.6 36.9 0.2 C C:THR75 3.7 40.5 0.8 C C:VAL76 3.8 36.1 0.2 CA C:VAL76 3.9 35.9 0.2 N C:GLY77 4.0 42.4 0.8 C C:VAL76 4.1 42.2 0.8 K C:K1135 4.1 38.1 0.1 OG1 C:THR75 4.2 39.3 0.8 N C:VAL76 4.2 36.4 0.2 CA C:GLY77 4.2 41.9 0.8 O C:HOH2019 4.4 45.8 0.2 CB C:THR75 4.4 39.5 0.8 O C:VAL76 4.5 42.4 0.8 CA C:VAL76 4.5 41.2 0.8 N C:VAL76 4.5 40.6 0.8 CA C:THR75 4.6 39.8 0.8 CA C:THR75 4.8 37.2 0.2 N C:GLY77 4.9 36.2 0.2 CB C:THR75 5.0 37.1 0.2

Potassium binding site 5 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1133 b:17.0 occ:0.05 K C:K1129 0.3 40.6 0.2 O C:HOH2019 1.9 45.8 0.2 O C:THR75 2.4 36.9 0.2 K C:K1132 2.5 28.5 0.1 OG1 C:THR75 2.6 39.3 0.8 O C:THR75 2.8 40.9 0.8 K C:K1131 3.0 69.0 0.2 CB C:THR75 3.3 39.5 0.8 OG1 C:THR75 3.4 36.8 0.2 C C:THR75 3.6 36.9 0.2 C C:THR75 3.8 40.5 0.8 CB C:THR75 3.8 37.1 0.2 O C:HOH2018 4.1 64.7 0.8 CA C:THR75 4.3 39.8 0.8 CA C:THR75 4.3 37.2 0.2 CAA C:HX01128 4.3 45.1 0.1 CAZ C:HX01128 4.3 45.1 0.1 CAA C:HX01128 4.4 44.1 0.1 CAZ C:HX01128 4.4 44.1 0.1 CG2 C:THR75 4.5 39.2 0.8 N C:VAL76 4.6 36.4 0.2 CA C:VAL76 4.8 35.9 0.2 NAQ C:HX01128 4.9 45.2 0.1 N C:VAL76 4.9 40.6 0.8 O C:THR74 4.9 38.1 1.0 NAQ C:HX01128 4.9 44.2 0.1

Potassium binding site 6 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1134 b:23.7 occ:0.05 K C:K1130 0.9 60.1 0.2 K C:K1135 2.1 38.1 0.1 O C:GLY77 2.4 42.8 0.8 O C:TYR78 2.5 37.8 0.2 O C:GLY77 3.1 36.5 0.2 O C:HOH2020 3.2 23.6 0.1 C C:GLY77 3.3 42.3 0.8 C C:TYR78 3.3 37.7 0.2 CA C:TYR78 3.9 37.4 0.2 CA C:GLY77 4.0 41.9 0.8 C C:TYR78 4.0 39.8 0.8 O C:TYR78 4.0 39.7 0.8 C C:GLY77 4.1 36.7 0.2 N C:TYR78 4.2 41.0 0.8 CA C:TYR78 4.3 39.8 0.8 N C:GLY79 4.3 38.8 1.0 N C:TYR78 4.5 37.1 0.2 O C:HOH2004 4.6 49.2 0.2 O C:VAL76 4.7 35.6 0.2 CA C:GLY79 4.7 37.9 1.0

Potassium binding site 7 out of 7 in the Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Potassium Channel Kcsa-Fab Complex with Tetraoctylammonium within 5.0Å range: probe atom residue distance (Å) B Occ C:K1135 b:38.1 occ:0.05 O C:HOH2020 1.1 23.6 0.1 K C:K1134 2.1 23.7 0.1 O C:GLY77 2.2 42.8 0.8 CA C:GLY77 2.8 41.9 0.8 O C:GLY77 2.9 36.5 0.2 C C:GLY77 2.9 42.3 0.8 K C:K1130 3.0 60.1 0.2 O C:VAL76 3.1 35.6 0.2 K C:K1131 3.6 69.0 0.2 C C:GLY77 3.7 36.7 0.2 O C:TYR78 4.0 37.8 0.2 N C:GLY77 4.0 42.4 0.8 N C:TYR78 4.1 41.0 0.8 K C:K1132 4.1 28.5 0.1 CA C:TYR78 4.3 37.4 0.2 C C:VAL76 4.3 36.1 0.2 N C:TYR78 4.3 37.1 0.2 C C:TYR78 4.3 37.7 0.2 O C:VAL76 4.4 42.4 0.8 C C:VAL76 4.6 42.2 0.8 CA C:GLY77 4.6 36.4 0.2 CA C:TYR78 4.9 39.8 0.8 N C:GLY77 5.0 36.2 0.2

M.J.Lenaeus, D.Burdette, T.Wagner, P.J.Focia, A.Gross. Structures of Kcsa in Complex with Symmetrical Quaternary Ammonium Compounds Reveal A Hydrophobic Binding Site. Biochemistry V. 53 5365 2014.
ISSN: ISSN 0006-2960
PubMed: 25093676
DOI: 10.1021/BI500525S