Potassium in PDB 2vlf: Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine

All present enzymatic activity of Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine:
4.1.99.2;

The structure of Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine, PDB code: 2vlf was solved by D.Milic, T.V.Demidkina, D.Matkovic-Calogovic, A.A.Antson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.89
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	132.846, 143.687, 59.618, 90.00, 90.00, 90.00
R / Rfree (%)	15.7 / 19.8

The binding sites of Potassium atom in the Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine (pdb code 2vlf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine, PDB code: 2vlf:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine within 5.0Å range: probe atom residue distance (Å) B Occ B:K1457 b:4.0 occ:1.00 O B:HOH2367 2.6 3.5 1.0 OE1 B:GLU69 2.7 20.0 1.0 O A:GLY52 2.8 20.9 1.0 O A:HOH2080 2.9 5.2 1.0 O A:ASN262 3.0 23.4 1.0 O A:HOH2274 3.1 5.1 1.0 O B:GLU69 3.2 22.4 1.0 CB B:GLU69 3.6 14.0 1.0 C A:GLY52 3.7 21.4 1.0 CD B:GLU69 3.8 16.4 1.0 C A:ASN262 3.9 20.5 1.0 C B:GLU69 3.9 18.0 1.0 CA B:GLU69 3.9 17.4 1.0 CA A:GLY52 4.0 20.9 1.0 CB A:ASN262 4.0 19.0 1.0 O B:HOH2122 4.1 9.0 1.0 CA A:ASN262 4.1 22.2 1.0 CG B:GLU69 4.3 16.1 1.0 CA B:ALA295 4.4 17.2 1.0 N B:GLY296 4.5 18.8 1.0 CE A:LYS256 4.7 21.6 1.0 N A:THR53 4.8 20.2 1.0 ND2 A:ASN262 4.8 18.7 1.0 O B:ALA70 4.9 18.8 1.0 O B:LEU294 4.9 20.4 1.0 CG A:ASN262 4.9 20.4 1.0 OE2 B:GLU69 4.9 18.4 1.0 CB B:ALA295 5.0 16.4 1.0 O A:SER51 5.0 20.7 1.0 C B:ALA295 5.0 19.7 1.0

Potassium binding site 2 out of 2 in the Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Quinonoid Intermediate of Citrobacter Freundii Tyrosine Phenol-Lyase Formed with Alanine within 5.0Å range: probe atom residue distance (Å) B Occ B:K1458 b:4.2 occ:1.00 O A:HOH2302 2.7 2.0 1.0 OE1 A:GLU69 2.8 17.6 1.0 O B:ASN262 2.8 19.5 1.0 O B:GLY52 2.9 20.3 1.0 O B:HOH2341 3.0 5.0 1.0 O B:HOH2100 3.0 6.4 1.0 O A:GLU69 3.3 20.0 1.0 C B:GLY52 3.7 21.1 1.0 CB A:GLU69 3.8 16.0 1.0 C B:ASN262 3.8 20.4 1.0 CD A:GLU69 3.9 16.8 1.0 CA B:GLY52 4.0 21.3 1.0 CB B:ASN262 4.1 22.2 1.0 CA A:GLU69 4.1 15.7 1.0 C A:GLU69 4.1 17.9 1.0 CA B:ASN262 4.1 19.5 1.0 CG A:GLU69 4.3 17.5 1.0 CA A:ALA295 4.3 19.7 1.0 O A:HOH2094 4.4 2.9 1.0 N A:GLY296 4.5 18.1 1.0 CE B:LYS256 4.8 19.9 1.0 N B:THR53 4.8 21.4 1.0 O A:LEU294 4.8 21.7 1.0 ND2 B:ASN262 4.9 21.8 1.0 O B:SER51 4.9 18.8 1.0 CB A:ALA295 4.9 17.5 1.0 OE2 A:GLU69 4.9 18.5 1.0 C A:ALA295 4.9 21.0 1.0 CG B:ASN262 5.0 20.0 1.0

D.Milic, T.V.Demidkina, N.G.Faleev, D.Matkovic-Calogovic, A.A.Antson. Insights Into the Catalytic Mechanism of Tyrosine Phenol-Lyase From X-Ray Structures of Quinonoid Intermediates. J.Biol.Chem. V. 283 29206 2008.
ISSN: ISSN 0021-9258
PubMed: 18715865
DOI: 10.1074/JBC.M802061200