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Potassium in PDB 2sgd: Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7

Enzymatic activity of Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7

All present enzymatic activity of Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7:
3.4.21.81;

Protein crystallography data

The structure of Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7, PDB code: 2sgd was solved by K.Huang, W.Lu, S.Anderson, M.Laskowski Jr., M.N.G.James, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.80
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 45.590, 54.610, 45.620, 90.00, 119.24, 90.00
R / Rfree (%) n/a / n/a

Potassium Binding Sites:

The binding sites of Potassium atom in the Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7 (pdb code 2sgd). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total only one binding site of Potassium was determined in the Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7, PDB code: 2sgd:

Potassium binding site 1 out of 1 in 2sgd

Go back to Potassium Binding Sites List in 2sgd
Potassium binding site 1 out of 1 in the Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Asp 18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Proteinase B at pH 10.7 within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K501

b:27.1
occ:1.00
O E:ALA192 2.5 3.7 1.0
O I:HOH86 2.6 22.6 1.0
O E:GLY218 3.0 19.0 1.0
O E:HOH596 3.0 22.6 1.0
O I:HOH71 3.1 26.2 1.0
OD1 I:ASP18 3.1 12.4 1.0
O I:HOH85 3.2 22.1 1.0
O E:HOH531 3.4 21.3 1.0
C E:ALA192 3.6 10.7 1.0
OD2 I:ASP18 3.7 14.7 1.0
CG I:ASP18 3.8 12.7 1.0
C E:GLY218 4.1 13.4 1.0
C E:SER217 4.1 11.2 1.0
O E:SER217 4.1 9.2 1.0
CA E:GLU192A 4.2 7.0 1.0
N E:GLU192A 4.3 5.3 1.0
CA E:SER217 4.4 10.1 1.0
C E:GLY216 4.4 11.3 1.0
O E:GLY216 4.4 10.1 1.0
N E:SER217 4.4 8.4 1.0
N E:GLY218 4.5 10.4 1.0
CA E:ALA192 4.6 5.3 1.0
N E:ALA192 4.7 3.2 1.0
CB E:ALA192 4.8 1.0 1.0
CA E:GLY218 4.9 13.6 1.0
CA E:ASN219 4.9 7.6 1.0
N E:ASN219 4.9 9.8 1.0
CD E:PRO192B 4.9 1.4 1.0
OD1 I:ASN36 5.0 18.1 1.0

Reference:

K.Huang, W.Lu, S.Anderson, M.Laskowski Jr., M.N.G.James. Recruitment of A Buried K+ Ion to Stabilize the Negative Charge of Ionized P1 in the Hydrophobic Pocket: Crystal Structures of GLU18, GLN18, ASP18 and ASN18 Variants of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces Griseus Protease B at Various Phs To Be Published.
Page generated: Mon Aug 12 06:57:21 2024

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