Potassium in PDB 2l8m: Reduced and Co-Bound Cytochrome P450CAM (CYP101A1)

All present enzymatic activity of Reduced and Co-Bound Cytochrome P450CAM (CYP101A1):
1.14.15.1;

The structure of Reduced and Co-Bound Cytochrome P450CAM (CYP101A1) also contains other interesting chemical elements:

Iron	(Fe)	1 atom
Chlorine	(Cl)	3 atoms

The binding sites of Potassium atom in the Reduced and Co-Bound Cytochrome P450CAM (CYP101A1) (pdb code 2l8m). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Reduced and Co-Bound Cytochrome P450CAM (CYP101A1), PDB code: 2l8m:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Reduced and Co-Bound Cytochrome P450CAM (CYP101A1)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Reduced and Co-Bound Cytochrome P450CAM (CYP101A1) within 5.0Å range: probe atom residue distance (Å) B Occ A:K418 b:0.0 occ:1.00 H1 A:HOH559 2.9 0.0 1.0 O A:HOH843 3.0 0.0 1.0 OD1 A:ASP153 3.1 0.0 1.0 O A:HOH559 3.3 0.0 1.0 H1 A:HOH843 3.4 0.0 1.0 O A:HOH571 3.5 0.0 1.0 H2 A:HOH843 3.5 0.0 1.0 H1 A:HOH571 3.7 0.0 1.0 H1 A:HOH1048 3.7 0.0 1.0 H2 A:HOH1048 3.7 0.0 1.0 H1 A:HOH2178 3.9 0.0 1.0 H2 A:HOH571 3.9 0.0 1.0 H2 A:HOH471 4.0 0.0 1.0 H1 A:HOH3247 4.1 0.0 1.0 CG A:ASP153 4.1 0.0 1.0 O A:HOH1048 4.1 0.0 1.0 H2 A:HOH559 4.2 0.0 1.0 HA A:CYS148 4.3 0.0 1.0 OD2 A:ASP153 4.4 0.0 1.0 O A:HOH2178 4.4 0.0 1.0 H2 A:HOH3247 4.5 0.0 1.0 O A:HOH3247 4.6 0.0 1.0 H2 A:HOH2178 4.7 0.0 1.0 O A:HOH471 4.7 0.0 1.0 HB2 A:CYS148 4.8 0.0 1.0 O A:HOH1496 4.9 0.0 1.0 O A:HOH2051 4.9 0.0 1.0 H A:ASN149 4.9 0.0 1.0

Potassium binding site 2 out of 4 in the Reduced and Co-Bound Cytochrome P450CAM (CYP101A1)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Reduced and Co-Bound Cytochrome P450CAM (CYP101A1) within 5.0Å range: probe atom residue distance (Å) B Occ A:K419 b:0.0 occ:1.00 O A:GLN46 2.5 0.0 1.0 O A:HOH1905 2.8 0.0 1.0 O A:HOH1865 2.8 0.0 1.0 H1 A:HOH1865 3.1 0.0 1.0 HB3 A:GLN46 3.2 0.0 1.0 HH22 A:ARG67 3.2 0.0 1.0 H2 A:HOH1865 3.3 0.0 1.0 C A:GLN46 3.4 0.0 1.0 H1 A:HOH1905 3.4 0.0 1.0 HH12 A:ARG67 3.5 0.0 1.0 H2 A:HOH1905 3.5 0.0 1.0 H2 A:HOH1886 3.6 0.0 1.0 HA A:GLU47 3.6 0.0 1.0 HB2 A:GLN46 3.8 0.0 1.0 CB A:GLN46 3.9 0.0 1.0 O A:HOH1912 3.9 0.0 1.0 H1 A:HOH1912 4.1 0.0 1.0 CA A:GLN46 4.2 0.0 1.0 N A:GLU47 4.2 0.0 1.0 NH2 A:ARG67 4.2 0.0 1.0 H2 A:HOH591 4.2 0.0 1.0 O A:HOH1886 4.3 0.0 1.0 NH1 A:ARG67 4.4 0.0 1.0 CA A:GLU47 4.5 0.0 1.0 H1 A:HOH1886 4.6 0.0 1.0 H2 A:HOH2409 4.7 0.0 1.0 O A:HOH1772 4.7 0.0 1.0 HB2 A:GLU329 4.7 0.0 1.0 HA A:GLN46 4.8 0.0 1.0 O A:HOH2409 4.8 0.0 1.0 CZ A:ARG67 4.8 0.0 1.0 O A:HOH591 4.8 0.0 1.0 H2 A:HOH1772 4.8 0.0 1.0 H2 A:HOH1912 4.8 0.0 1.0 HH21 A:ARG67 4.8 0.0 1.0 H A:GLU329 5.0 0.0 1.0 H A:SER48 5.0 0.0 1.0

Potassium binding site 3 out of 4 in the Reduced and Co-Bound Cytochrome P450CAM (CYP101A1)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Reduced and Co-Bound Cytochrome P450CAM (CYP101A1) within 5.0Å range: probe atom residue distance (Å) B Occ A:K420 b:0.0 occ:1.00 O A:GLU76 2.7 0.0 1.0 O A:HOH2961 3.0 0.0 1.0 H2 A:HOH3552 3.1 0.0 1.0 H2 A:HOH2961 3.2 0.0 1.0 HB3 A:GLU76 3.4 0.0 1.0 C A:GLU76 3.5 0.0 1.0 H1 A:HOH3570 3.5 0.0 1.0 H1 A:HOH3552 3.7 0.0 1.0 O A:HOH3570 3.7 0.0 1.0 O A:HOH3552 3.7 0.0 1.0 H1 A:HOH2961 3.8 0.0 1.0 HA A:ASP77 3.8 0.0 1.0 H2 A:HOH3570 4.0 0.0 1.0 N A:ASP77 4.1 0.0 1.0 H1 A:HOH3460 4.2 0.0 1.0 CB A:GLU76 4.4 0.0 1.0 CA A:GLU76 4.4 0.0 1.0 CA A:ASP77 4.4 0.0 1.0 H1 A:HOH3637 4.5 0.0 1.0 CG A:ASP77 4.6 0.0 1.0 OD1 A:ASP77 4.6 0.0 1.0 HA A:GLU76 4.6 0.0 1.0 OD2 A:ASP77 4.6 0.0 1.0 H A:ASP77 4.7 0.0 1.0 H2 A:HOH2481 4.8 0.0 1.0 HG2 A:GLU76 4.9 0.0 1.0 O A:HOH3460 4.9 0.0 1.0 H A:TYR78 4.9 0.0 1.0

Potassium binding site 4 out of 4 in the Reduced and Co-Bound Cytochrome P450CAM (CYP101A1)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Reduced and Co-Bound Cytochrome P450CAM (CYP101A1) within 5.0Å range: probe atom residue distance (Å) B Occ A:K421 b:0.0 occ:1.00 OE1 A:GLU156 2.7 0.0 1.0 OE2 A:GLU156 2.8 0.0 1.0 H2 A:HOH1044 2.9 0.0 1.0 O A:HOH1012 2.9 0.0 1.0 CD A:GLU156 3.0 0.0 1.0 O A:HOH1044 3.0 0.0 1.0 O A:HOH1811 3.1 0.0 1.0 H1 A:HOH1044 3.1 0.0 1.0 H1 A:HOH1811 3.1 0.0 1.0 HB2 A:GLU152 3.3 0.0 1.0 H2 A:HOH1012 3.4 0.0 1.0 HB3 A:GLU152 3.5 0.0 1.0 H1 A:HOH1012 3.7 0.0 1.0 H1 A:HOH1702 3.8 0.0 1.0 HA A:GLU152 3.9 0.0 1.0 CB A:GLU152 4.0 0.0 1.0 H2 A:HOH1811 4.0 0.0 1.0 HB3 A:GLU156 4.2 0.0 1.0 O A:HOH2142 4.3 0.0 1.0 CG A:GLU156 4.4 0.0 1.0 H2 A:HOH2142 4.4 0.0 1.0 O A:HOH1702 4.5 0.0 1.0 CA A:GLU152 4.6 0.0 1.0 H2 A:HOH1702 4.7 0.0 1.0 H1 A:HOH2142 4.7 0.0 1.0 HG2 A:GLU156 4.8 0.0 1.0 CB A:GLU156 4.8 0.0 1.0 HD3 A:PRO157 4.9 0.0 1.0 OE1 A:GLU152 5.0 0.0 1.0

E.K.Asciutto, M.Dang, S.S.Pochapsky, J.D.Madura, T.C.Pochapsky. Experimentally Restrained Molecular Dynamics Simulations For Characterizing the Open States of Cytochrome P450(Cam). Biochemistry V. 50 1664 2011.
ISSN: ISSN 0006-2960
PubMed: 21265500
DOI: 10.1021/BI101820D