Potassium in the structure of Crystallographic And Kinetic Studies Of Human Mitochondrial Acetoacetyl-Coa Thiolase (T2): the Importance of Potassium And Chloride For Its Structure and Function (pdb 2ib9)

The binding sites of Potassium atom in the structure of Crystallographic And Kinetic Studies Of Human Mitochondrial Acetoacetyl-Coa Thiolase (T2): the Importance of Potassium And Chloride For Its Structure and Function (pdb code 2ib9). This binding sites where shown with 5.0 Angstroms radius around Potassium atom. The 2ib9 structure was solved by A.M.HAAPALAINEN, R.K.WIERENGA, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 73.7-2.0 Space group P1211 a (A) 75.749 b (A) 107.440 c (A) 102.326 alpha (°) 90.00 beta (°) 103.06 gamma (°) 90.00 Rfactor (%) 15.6 Rfree (%) 19.9 Potassium Binding Sites: Potassium binding site 1 out of 4 in 2ib9 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Potassium in the PDB 2ib9. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Tyr219, A: Ala280, A: Ala281, A: Asn282, A: Ala283, A: Ser284, A: Thr285, A: Val381, A: Ser382, A: Hoh5037, A: Hoh5044, A: Hoh5101, A: Hoh5215, conact list: Atom Atom Distance (A) K CE2 A:Tyr219 4.10 K CZ A:Tyr219 3.86 K OH A:Tyr219 2.80 K O A:Ala280 2.97 K C A:Ala280 4.02 K O A:Ala281 2.70 K N A:Ala281 4.37 K C A:Ala281 3.40 K CB A:Ala281 4.98 K CA A:Ala281 3.72 K N A:Asn282 4.45 K C A:Asn282 4.91 K CA A:Asn282 4.97 K O A:Ala283 2.72 K N A:Ala283 4.49 K C A:Ala283 3.95 K CA A:Ala283 4.86 K N A:Ser284 4.83 K C A:Ser284 4.74 K CA A:Ser284 4.76 K N A:Thr285 4.96 K CG2 A:Thr285 3.84 K O A:Val381 2.77 K CB A:Val381 4.86 K C A:Val381 3.89 K CG1 A:Val381 4.08 K CA A:Val381 4.47 K N A:Ser382 5.00 K O A:Hoh5037 4.37 K O A:Hoh5044 4.28 K O A:Hoh5101 2.80 K O A:Hoh5215 4.93 interactive model: Potassium binding site 2 out of 4 in 2ib9 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Potassium in the PDB 2ib9. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Tyr219, B: Ala280, B: Ala281, B: Asn282, B: Ala283, B: Ser284, B: Thr285, B: Val381, B: Hoh5042, B: Hoh5050, B: Hoh5071, B: Hoh5198, conact list: Atom Atom Distance (A) K CE2 B:Tyr219 4.14 K CZ B:Tyr219 3.89 K OH B:Tyr219 2.84 K O B:Ala280 3.06 K C B:Ala280 4.12 K O B:Ala281 2.83 K N B:Ala281 4.46 K C B:Ala281 3.49 K CB B:Ala281 4.99 K CA B:Ala281 3.78 K N B:Asn282 4.53 K O B:Ala283 2.71 K N B:Ala283 4.56 K C B:Ala283 3.94 K CA B:Ala283 4.88 K N B:Ser284 4.79 K C B:Ser284 4.74 K CA B:Ser284 4.75 K N B:Thr285 4.88 K CG2 B:Thr285 3.78 K O B:Val381 2.78 K CB B:Val381 4.86 K C B:Val381 3.92 K CG1 B:Val381 4.03 K CA B:Val381 4.50 K O B:Hoh5042 4.34 K O B:Hoh5050 4.27 K O B:Hoh5071 2.84 K O B:Hoh5198 4.86 interactive model: Potassium binding site 3 out of 4 in 2ib9 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Potassium in the PDB 2ib9. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: Tyr219, C: Ala280, C: Ala281, C: Asn282, C: Ala283, C: Ser284, C: Thr285, C: Val381, C: Hoh5009, C: Hoh5050, C: Hoh5054, conact list: Atom Atom Distance (A) K CE2 C:Tyr219 4.17 K CZ C:Tyr219 3.90 K OH C:Tyr219 2.82 K O C:Ala280 3.01 K C C:Ala280 4.07 K O C:Ala281 2.76 K N C:Ala281 4.41 K C C:Ala281 3.45 K CA C:Ala281 3.77 K N C:Asn282 4.48 K C C:Asn282 4.98 K O C:Ala283 2.75 K N C:Ala283 4.50 K C C:Ala283 3.98 K CA C:Ala283 4.90 K N C:Ser284 4.85 K C C:Ser284 4.78 K CA C:Ser284 4.76 K N C:Thr285 4.99 K CG2 C:Thr285 3.79 K O C:Val381 2.84 K CB C:Val381 4.99 K C C:Val381 4.00 K CG1 C:Val381 4.22 K CA C:Val381 4.62 K O C:Hoh5009 4.45 K O C:Hoh5050 2.74 K O C:Hoh5054 3.99 interactive model: Potassium binding site 4 out of 4 in 2ib9 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 4 of Potassium in the PDB 2ib9. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: D: Tyr219, D: Ala280, D: Ala281, D: Asn282, D: Ala283, D: Ser284, D: Thr285, D: Val381, D: Ser382, D: Hoh5042, D: Hoh5064, D: Hoh5066, conact list: Atom Atom Distance (A) K CE2 D:Tyr219 4.37 K CZ D:Tyr219 3.97 K OH D:Tyr219 2.87 K O D:Ala280 3.04 K C D:Ala280 4.14 K O D:Ala281 3.00 K N D:Ala281 4.56 K C D:Ala281 3.63 K CA D:Ala281 3.99 K N D:Asn282 4.55 K O D:Ala283 2.77 K N D:Ala283 4.48 K C D:Ala283 4.01 K CA D:Ala283 4.93 K N D:Ser284 4.88 K C D:Ser284 4.82 K CA D:Ser284 4.81 K N D:Thr285 4.90 K CG2 D:Thr285 3.80 K O D:Val381 2.76 K CB D:Val381 4.83 K C D:Val381 3.88 K CG1 D:Val381 4.08 K CA D:Val381 4.46 K N D:Ser382 4.99 K O D:Hoh5042 2.89 K O D:Hoh5064 4.30 K O D:Hoh5066 4.47 interactive model: