Potassium in PDB 2gr6: Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A)

All present enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A):
1.14.15.1;

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A), PDB code: 2gr6 was solved by Z.Rao, L.L.Wong, F.Xu, S.G.Bell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.00 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.664, 62.356, 95.098, 90.00, 90.37, 90.00
R / Rfree (%)	18.7 / 24.4

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) (pdb code 2gr6). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A), PDB code: 2gr6:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) within 5.0Å range: probe atom residue distance (Å) B Occ A:K1418 b:33.8 occ:1.00 O A:GLU84 2.8 28.6 1.0 O A:PHE96 2.9 26.5 1.0 O A:GLY93 3.0 25.8 1.0 O A:GLU94 3.0 25.6 1.0 O A:HOH1637 3.2 45.7 1.0 C A:GLU94 3.5 25.5 1.0 CA A:GLU94 3.7 25.8 1.0 C A:PHE96 3.9 26.4 1.0 C A:GLU84 3.9 29.2 1.0 C A:GLY93 4.0 26.4 1.0 OE2 A:GLU84 4.0 47.5 1.0 O A:HOH1553 4.1 35.8 1.0 N A:PHE96 4.2 24.9 1.0 N A:GLU94 4.3 26.0 1.0 N A:ALA95 4.4 24.1 1.0 CA A:PHE96 4.5 25.7 1.0 CA A:CYS85 4.6 27.5 1.0 C A:ALA95 4.6 25.5 1.0 CB A:PHE96 4.6 23.3 1.0 N A:CYS85 4.7 27.4 1.0 N A:ASP97 4.9 27.3 1.0 CA A:GLU84 4.9 29.1 1.0 CD A:GLU84 5.0 43.4 1.0 CB A:GLU94 5.0 26.6 1.0 CA A:ASP97 5.0 28.2 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) within 5.0Å range: probe atom residue distance (Å) B Occ B:K2418 b:56.1 occ:1.00 O B:GLU84 2.9 40.3 1.0 O B:PHE96 3.1 34.9 1.0 O B:GLU94 3.1 37.9 1.0 O B:GLY93 3.3 38.4 1.0 C B:GLU94 3.7 37.4 1.0 CA B:GLU94 3.9 39.9 1.0 C B:GLU84 4.0 40.2 1.0 C B:PHE96 4.2 34.5 1.0 C B:GLY93 4.3 38.6 1.0 N B:GLU94 4.6 40.0 1.0 N B:PHE96 4.7 34.3 1.0 N B:ALA95 4.8 35.6 1.0 N B:CYS85 4.8 39.4 1.0 CA B:CYS85 4.8 40.0 1.0 CA B:GLU84 4.9 42.5 1.0 C B:ALA95 5.0 35.0 1.0 CA B:PHE96 5.0 34.3 1.0

F.Xu, S.G.Bell, G.Taylor, J.C.Green, Z.Rao, L.L.Wong. Pentachlorobenzene Oxidation By Engineered Cytochrome P450CAM: Substrate Binding and the Mechanism of Aromatic C-H Bond Oxidation To Be Published.