Atomistry » Potassium » PDB 2fxi-2hw8 » 2gqx
Atomistry »
  Potassium »
    PDB 2fxi-2hw8 »
      2gqx »

Potassium in PDB 2gqx: Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene

Enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene

All present enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene:
1.14.15.1;

Protein crystallography data

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene, PDB code: 2gqx was solved by Z.Rao, L.L.Wong, F.Xu, S.G.Bell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 26.00 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 67.033, 62.037, 95.004, 90.00, 89.64, 90.00
R / Rfree (%) 19.3 / 23.6

Other elements in 2gqx:

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene also contains other interesting chemical elements:

Iron (Fe) 2 atoms
Chlorine (Cl) 5 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene (pdb code 2gqx). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene, PDB code: 2gqx:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 2gqx

Go back to Potassium Binding Sites List in 2gqx
Potassium binding site 1 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K1418

b:16.9
occ:1.00
O A:PHE96 2.8 14.2 1.0
O A:GLU84 2.8 18.6 1.0
O A:GLY93 3.0 21.2 1.0
O A:GLU94 3.0 17.0 1.0
C A:GLU94 3.5 18.8 1.0
CA A:GLU94 3.8 19.6 1.0
C A:PHE96 3.8 14.9 1.0
O A:HOH1611 3.9 34.9 1.0
C A:GLU84 3.9 19.6 1.0
C A:GLY93 4.0 19.7 1.0
OE2 A:GLU84 4.1 37.7 1.0
N A:PHE96 4.2 17.0 1.0
N A:GLU94 4.4 18.6 1.0
N A:ALA95 4.5 18.6 1.0
CA A:PHE96 4.5 15.2 1.0
C A:ALA95 4.6 17.7 1.0
CA A:CYS85 4.6 17.0 1.0
CB A:PHE96 4.7 15.1 1.0
N A:CYS85 4.7 17.5 1.0
N A:ASP97 4.9 15.2 1.0
CA A:GLU84 4.9 20.6 1.0
CA A:ASP97 5.0 15.7 1.0

Potassium binding site 2 out of 2 in 2gqx

Go back to Potassium Binding Sites List in 2gqx
Potassium binding site 2 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/L244A/V247L/C334A) with Pentachlorobenzene within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K2418

b:24.1
occ:1.00
O B:PHE96 2.9 23.4 1.0
O B:GLU84 2.9 28.6 1.0
O B:GLY93 3.0 26.3 1.0
O B:HOH2640 3.1 24.8 1.0
O B:GLU94 3.2 24.3 1.0
O B:HOH2639 3.3 39.7 1.0
C B:GLU94 3.6 25.7 1.0
CA B:GLU94 3.8 26.1 1.0
C B:PHE96 3.9 21.8 1.0
C B:GLU84 3.9 27.4 1.0
C B:GLY93 4.1 25.3 1.0
N B:PHE96 4.3 22.1 1.0
N B:GLU94 4.5 25.6 1.0
N B:ALA95 4.5 23.8 1.0
CA B:PHE96 4.5 22.1 1.0
C B:ALA95 4.5 22.8 1.0
CA B:CYS85 4.6 28.0 1.0
N B:CYS85 4.7 27.7 1.0
CB B:PHE96 4.7 23.3 1.0
N B:ASP97 4.8 21.7 1.0
O B:ALA95 4.9 21.0 1.0
CA B:GLU84 4.9 27.3 1.0
CA B:ALA95 5.0 24.0 1.0
CA B:ASP97 5.0 21.6 1.0

Reference:

F.Xu, S.G.Bell, Z.Rao, L.L.Wong. Structure-Activity Correlations in Pentachlorobenzene Oxidation By Engineered Cytochrome P450CAM Protein Eng.Des.Sel. V. 20 473 2007.
ISSN: ISSN 1741-0126
PubMed: 17962225
DOI: 10.1093/PROTEIN/GZM028
Page generated: Sun Dec 13 23:10:03 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy