Potassium in the structure of Structure of the Mnme G-Domain in Complex With Gdp*ALF4-, MG2+ and K+ (pdb 2gj8)

The binding sites of Potassium atom in the structure of Structure of the Mnme G-Domain in Complex With Gdp*ALF4-, MG2+ and K+ (pdb code 2gj8). This binding sites where shown with 5.0 Angstroms radius around Potassium atom. The 2gj8 structure was solved by A.SCRIMA, A.WITTINGHOFER, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 20.0-1.7 Space group P1211 a (A) 57.540 b (A) 70.227 c (A) 91.300 alpha (°) 90.00 beta (°) 95.51 gamma (°) 90.00 Rfactor (%) 20.5 Rfree (%) 25.6 Potassium Binding Sites: Potassium binding site 1 out of 4 in 2gj8 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Potassium in the PDB 2gj8. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asn226, A: Thr245, A: Asp246, A: Ile247, A: Ala248, A: Gly249, A: Thr250, A: Alf601, A: Mg602, A: Gdp650, A: Hoh654, conact list: Atom Atom Distance (A) K N A:Asn226 4.55 K CB A:Asn226 4.75 K ND2 A:Asn226 4.74 K OD1 A:Asn226 2.66 K CG A:Asn226 3.86 K CA A:Asn226 4.39 K O A:Thr245 2.86 K N A:Thr245 4.33 K OG1 A:Thr245 4.43 K C A:Thr245 3.99 K CA A:Thr245 4.75 K N A:Asp246 4.93 K C A:Asp246 4.69 K O A:Ile247 2.75 K N A:Ile247 4.37 K C A:Ile247 3.71 K CA A:Ile247 4.63 K N A:Ala248 4.44 K C A:Ala248 4.93 K CA A:Ala248 4.46 K N A:Gly249 4.77 K N A:Thr250 4.41 K CB A:Thr250 3.74 K CG2 A:Thr250 4.82 K OG1 A:Thr250 3.35 K CA A:Thr250 4.73 K F4 A:Alf601 3.80 K F3 A:Alf601 4.39 K F2 A:Alf601 2.58 K AL A:Alf601 3.69 K MG A:Mg602 4.97 K O3B A:Gdp650 4.50 K PA A:Gdp650 3.77 K O2A A:Gdp650 2.79 K PB A:Gdp650 3.92 K C5' A:Gdp650 3.84 K O5' A:Gdp650 4.26 K O2B A:Gdp650 2.98 K O3A A:Gdp650 3.75 K O A:Hoh654 3.63 interactive model: Potassium binding site 2 out of 4 in 2gj8 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Potassium in the PDB 2gj8. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Asn226, B: Thr245, B: Asp246, B: Ile247, B: Ala248, B: Gly249, B: Thr250, B: Alf611, B: Mg612, B: Gdp651, B: Hoh655, conact list: Atom Atom Distance (A) K N B:Asn226 4.43 K CB B:Asn226 4.69 K ND2 B:Asn226 4.74 K OD1 B:Asn226 2.65 K CG B:Asn226 3.84 K CA B:Asn226 4.30 K O B:Thr245 2.82 K N B:Thr245 4.39 K OG1 B:Thr245 4.49 K C B:Thr245 3.91 K CA B:Thr245 4.74 K N B:Asp246 4.77 K C B:Asp246 4.56 K CA B:Asp246 4.79 K O B:Ile247 2.91 K N B:Ile247 4.22 K C B:Ile247 3.74 K CA B:Ile247 4.64 K N B:Ala248 4.42 K C B:Ala248 4.97 K CA B:Ala248 4.47 K N B:Gly249 4.80 K N B:Thr250 4.36 K CB B:Thr250 3.64 K CG2 B:Thr250 4.72 K OG1 B:Thr250 3.36 K CA B:Thr250 4.63 K F4 B:Alf611 2.46 K F2 B:Alf611 3.62 K AL B:Alf611 3.60 K F1 B:Alf611 4.37 K MG B:Mg612 4.78 K O3B B:Gdp651 4.54 K PA B:Gdp651 3.74 K O2A B:Gdp651 2.72 K PB B:Gdp651 3.81 K C5' B:Gdp651 3.84 K O5' B:Gdp651 4.27 K O2B B:Gdp651 2.91 K O3A B:Gdp651 3.56 K O B:Hoh655 3.69 interactive model: Potassium binding site 3 out of 4 in 2gj8 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Potassium in the PDB 2gj8. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: Asn226, C: Thr245, C: Asp246, C: Ile247, C: Ala248, C: Gly249, C: Thr250, C: Alf621, C: Mg622, C: Gdp652, C: Hoh654, conact list: Atom Atom Distance (A) K N C:Asn226 4.46 K CB C:Asn226 4.77 K ND2 C:Asn226 4.73 K OD1 C:Asn226 2.70 K CG C:Asn226 3.88 K CA C:Asn226 4.40 K O C:Thr245 2.79 K N C:Thr245 4.39 K OG1 C:Thr245 4.40 K C C:Thr245 3.90 K CA C:Thr245 4.72 K N C:Asp246 4.79 K C C:Asp246 4.53 K CA C:Asp246 4.80 K O C:Ile247 2.88 K N C:Ile247 4.22 K C C:Ile247 3.71 K CA C:Ile247 4.57 K N C:Ala248 4.39 K C C:Ala248 4.92 K CA C:Ala248 4.46 K N C:Gly249 4.74 K N C:Thr250 4.39 K CB C:Thr250 3.68 K CG2 C:Thr250 4.76 K OG1 C:Thr250 3.30 K CA C:Thr250 4.69 K F4 C:Alf621 2.60 K F2 C:Alf621 3.68 K AL C:Alf621 3.60 K F1 C:Alf621 4.28 K MG C:Mg622 4.98 K O3B C:Gdp652 2.98 K PA C:Gdp652 3.84 K O2A C:Gdp652 2.93 K O1B C:Gdp652 4.55 K PB C:Gdp652 3.90 K C5' C:Gdp652 3.96 K O5' C:Gdp652 4.37 K O3A C:Gdp652 3.69 K O C:Hoh654 3.76 interactive model: Potassium binding site 4 out of 4 in 2gj8 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 4 of Potassium in the PDB 2gj8. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: D: Asn226, D: Thr245, D: Asp246, D: Ile247, D: Ala248, D: Gly249, D: Thr250, D: Alf631, D: Mg632, D: Gdp653, D: Hoh658, conact list: Atom Atom Distance (A) K N D:Asn226 4.46 K CB D:Asn226 4.76 K ND2 D:Asn226 4.68 K OD1 D:Asn226 2.69 K CG D:Asn226 3.85 K CA D:Asn226 4.38 K O D:Thr245 2.75 K N D:Thr245 4.45 K OG1 D:Thr245 4.46 K C D:Thr245 3.91 K CA D:Thr245 4.75 K N D:Asp246 4.78 K C D:Asp246 4.51 K CA D:Asp246 4.71 K O D:Ile247 2.83 K N D:Ile247 4.23 K C D:Ile247 3.71 K CA D:Ile247 4.56 K N D:Ala248 4.42 K CA D:Ala248 4.47 K N D:Gly249 4.77 K N D:Thr250 4.44 K CB D:Thr250 3.78 K CG2 D:Thr250 4.83 K OG1 D:Thr250 3.31 K CA D:Thr250 4.76 K F4 D:Alf631 3.75 K F3 D:Alf631 4.33 K F2 D:Alf631 2.55 K AL D:Alf631 3.57 K MG D:Mg632 4.98 K O3B D:Gdp653 4.46 K PA D:Gdp653 3.77 K O2A D:Gdp653 2.94 K PB D:Gdp653 3.87 K C5' D:Gdp653 3.86 K C4' D:Gdp653 4.99 K O5' D:Gdp653 4.36 K O2B D:Gdp653 2.88 K O3A D:Gdp653 3.62 K O D:Hoh658 3.73 interactive model: